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Citations for Electron Microscopy Sciences :
201 - 250 of 7095 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed 1x in PBS and then fixed for 10 minutes at room temperature in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). Matrix immunostaining was performed using the same procedure and antibodies as described previously.55
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bioRxiv - Molecular Biology 2024Quote: ... 10 nm BSA Gold Tracer (EMS, Hatfield, PA, USA) was added to the grid at a 5:1 ratio (chromosome:gold) ...
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bioRxiv - Neuroscience 2024Quote: ... and subsequently embedded in 100% Araldite resin solution (Araldite: DDSA: DMP30, 12:9:1, Electron Microscopy Sciences) and baked overnight at 60°C ...
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bioRxiv - Neuroscience 2024Quote: ... mice were perfused with phosphate-buffered saline (PBS, warmed up to 37 °C) with heparin solution (20 units / mL) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 µL sample was spotted onto a glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences CF300-Cu-50), incubated for two minutes then gently blotted onto filter paper to remove excess solution ...
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bioRxiv - Immunology 2024Quote: ... the cells were fixed with paraformaldehyde (Electron Microscopy Sciences). Cell fixation was performed with 4% PFA in PBS for 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μl of the purified G6PDH-OpcA sample at a protein concentration of approximately 1 mg/ml were applied to glow-discharged C-Flat grids (R1.2/1.3 3Cu-50) (EMS) and directly plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Neurons were washed once with 500ul of cold PBS and fixated with PBS + PFA (4:100, Electron Microscopy Sciences) for 10 min at 4°C ...
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bioRxiv - Genomics 2024Quote: Liver tissue sections (3-5 mm thickness) were fixed by submerging in fresh 4% v/v paraformaldehyde (PFA; EMS 15710) in 1X Phosphate Buffered Saline (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were then deposited onto 400 mesh carbon-coated TEM grids (EMS, ultra-thin) and negatively stained with Uranyl Acetate (1% ...
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bioRxiv - Plant Biology 2024Quote: Seeds of the pen3-4 (Stein et al., 2006) mutant were mutagenized by treatment with 0.24% ethyl-methansulfonate (EMS) for 16h (Normanly et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Samples of 2.5 µL were applied to a C-flat 300 mesh 1.2/1.3 copper grids (Electron Microscopy Sciences), glow-discharged in the presence of amylamine at 25 mA for 45 s ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were mounted on slides with Fluoromount G (EMS). Primary antibodies used for immunocytochemistry in this study are chicken anti-GFP (5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Animals were sacrificed 21 days after birth (P21) by terminal perfusion of 4% paraformaldehyde (PFA, Electron Microscopy Sciences) followed by overnight post-fixation in 4% PFA.
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bioRxiv - Neuroscience 2024Quote: Primary culture - Cells were fixed for 10 minutes at room temperature in 4% (w/v) paraformaldehyde (PFA, EMS) in PBS (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Neuroscience 2024Quote: ... The dorsal DG was micro-dissected using a disposable tissue punch tool (Electron Microscopy Sciences; diameter: 0.75 mm) directly into a 1.5ml microcentrifuge tube ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were collected onto Synaptek Be-Cu slot grids (Electron Microscopy Sciences or Ted Pella) that were coated with Pioloform (Ted Pella ...
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bioRxiv - Neuroscience 2024Quote: Mice were deeply anesthetized with a mixture of Ketamine/Xylazine and perfused transcardially with saline followed by 4% paraformaldehyde (PFA; Electron Microscopy Science). Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... Contrast was enhanced using 1% uranyl acetate in 70% ETOH within the dehydration series (EMS, Cat 22400). Sections were then treated with propylene oxide ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed thoroughly in 0.1M PBS, followed by a series of washes in Enhancement Conditioning Solution (1X concentration per package instructions, Electron Microscopy Science (EMS), Cat 25830 ...
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bioRxiv - Neuroscience 2024Quote: ... a section from the thoracic part of the spines were embedded in an 8×8 mm tissue embedding mold (Electron microscopy sciences, 70180) by freezing in Tissue-Tek OCT (Sakura ...
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bioRxiv - Neuroscience 2024Quote: ... FluoroGel (Electron Microscopy Sciences) was used to mount coverslips over the sections.
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bioRxiv - Neuroscience 2024Quote: ... followed by 4% paraformaldehyde (PFA) in 1x PBS (Electron Microscopy Sciences). Brains were post fixed in 4% PFA overnight at 4°C and afterwards transferred to 30% sucrose ...
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bioRxiv - Neuroscience 2024Quote: ... a 5mm coverslip attached to a 3mm coverslip (Electron microscopy sciences) by UV glue (Norland Optical Adhesive ...
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bioRxiv - Neuroscience 2024Quote: Retinas were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 30 minutes at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... with each incubation lasting 24 hours at room temperature (Spurr Resin kit, Electron Microscopy Science, 14300). Lumbricals were embedded in 100% Spurr and polymerized at 65°C for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed and coverslipped with Fluoro-Gel Mounting Medium with Tris Buffer (catalog #17985-10, Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2024Quote: Neurons were washed 3x in PBS and then fixed in 4% PFA (EMS 15710), 4% sucrose in PBS at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Temporal bones were immediately removed and fixed via perfusion of 1 ml cold 4% PFA (Electron Microscopy Science) in 1X PBS through the oval and round windows ...
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bioRxiv - Neuroscience 2024Quote: Mice were placed under isoflurane anesthetics until loss of pain reflex and transcardially perfused with filtered ice-cold PBS then 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were cryoprotected in 30% sucrose ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then rinsed with 0.1 M cacodylate buffer and fixed with 1 % osmium tetroxide (Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and then mounted in Entellan mounting medium (EMS, Hatfield, USA). Reconstituted tiles of the whole nerves were imaged at ×63 magnification ...
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bioRxiv - Neuroscience 2024Quote: ... diluted in PHEM buffer (0.1 M; pH 7.4) (Electron Microscopy Sciences # 11163). Sciatic and sural nerves were dissected and fixed in 4% PFA + 2.5% glutaraldehyde (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: Mice were perfused with 20 mL 1XPBS followed by 15mL of 4% PFA (Electron Microscopy Sciences # 15172) diluted in PHEM buffer (0.1 M ...
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bioRxiv - Neuroscience 2024Quote: ... cells were placed in glass slides using Fluoro-Gel II with DAPI (Electron Microscopy Sciences; 17985-51). Cells transfected with P1-HA-IRES-GFP (Wild-Type or any of the truncated version ...
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bioRxiv - Neuroscience 2024Quote: ... silver sections were collected onto Formvar-coated copper slot grids (EMS) and examined on a JEOL JEM 1011 transmission electron microscope (JEOL Ltd. ...
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bioRxiv - Neuroscience 2024Quote: ... and employing a different resin mixture (Electron Microscopy Sciences [EMS], Hatfield, PA, USA) containing EMbed 812 ...
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bioRxiv - Neuroscience 2024Quote: ... and employing a different resin mixture (Electron Microscopy Sciences [EMS], Hatfield, PA, USA) containing EMbed 812 ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with a mixture of 4% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences, EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with a mixture of 4% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences, EMS) in 0.1 M sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2024Quote: ... A standard rOTO en bloc staining protocol39 was applied including postfixation in 2% osmium tetroxide (EMS), 1.5% potassium ferricyanide (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Sciatic nerves were removed and fixed with 2% glutaraldehyde (Electron Microscopy Science) in 0.12 M phosphate buffer ...
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bioRxiv - Neuroscience 2024Quote: ... post fixed with 1% osmium tetroxide (Electron Microscopy Science), and embedded in Epon (Epoxy Embedding Medium kit ...
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bioRxiv - Neuroscience 2024Quote: ... and 30 mL 4% paraformaldehyde (15714, Electron Microscopy Sciences) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... followed by ice-cold EM fixative (1.25% glutaraldehyde [Electron Microscopy Sciences, 16220], 2% paraformaldehyde [Electron Microscopy Sciences, 19210], 0.1M sodium cacodylate buffer). Brains were removed and stored for 8 days in EM fixative at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by ice-cold EM fixative (1.25% glutaraldehyde [Electron Microscopy Sciences, 16220] ...
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bioRxiv - Neuroscience 2024Quote: P14 mice were euthanized and perfused transcardially with ice-cold Phosphate Buffered Saline (PBS) followed by ice-cold 4% paraformaldehyde (Electron Microscopy Sciences) diluted in water ...
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bioRxiv - Neuroscience 2024Quote: ... fixed in 4% formaldehyde (PFA, EMS, #15713) in HBSS for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% sodium azide (1hr rinse, 3 rinses) and was mounted on glass slides in anti-fade media (Citifluor, Electron Microscopy Sciences) and coverslipped ...