Labshake search
Citations for Electron Microscopy Sciences :
1 - 50 of 7233 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Samples were transitioned from ethanol to infiltration with propylene oxide and embedded in Embed-812 hard resin (Electron Microscopy Sciences, Hatfield, PA). Blocks were sectioned at 50 to 60 ριm and stained with uranyl acetate and Reynolds’ lead citrate ...
-
bioRxiv - Cancer Biology 2024Quote: ... pH 7.4) and dehydrated using a series of ethanol solutions followed by incubation in hexamethyldisilazane (Electron Microscopy Sciences) and coated with carbon using a sputter coater (Desk V ...
-
bioRxiv - Cell Biology 2024Quote: ... and fixed by immersion in Modified Karnovsky’s Fixative (2% Paraformaldehyde and 2.5% Glutaraldehyde in 0.1 M Sodium Cacodylate Buffer, pH 7.4 (Electron Microscopy Sciences (Hatfield, PA), #15960-01) ...
-
bioRxiv - Cell Biology 2024Quote: ... and embedded in epoxy resin Embed812 (Electron Microscopy Science). Ultrathin sections (70 nm ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, #50-980487) at room temperature for 30 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... tissue was then dehydrated by incubation in a graded ethanol series followed by incubation in 1:1 ethanol:Spurr resin (Electron Microscopy Sciences; Hatfield, PA, USA) overnight ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were fixed for 30 min with 3% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized for 10 min with 0.1 % Triton X-100 and blocked for 30 min with 10% goat serum before incubation with specific primary antibodies for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... stained with uranyl acetate (Electron Microscopy Sciences, #22400) and Reynolds’s lead citrate and observed into a FEI Tecnai G2 Spirit Transmission Electron Microscope (TEM ...
-
bioRxiv - Molecular Biology 2024Quote: ... CF-400 1.2/1.3 grids (Electron Microscopy Sciences) were glow discharged for 60 seconds at 15 mAmps (PELCO easiGlow™ Glow Discharge Cleaning System).
-
bioRxiv - Cell Biology 2024Quote: ... Extracellular parasites were flushed out with excess of cold PBS before fixation of the HFF samples with 3.2% paraformaldehyde (PFA, 15714, Electron Microscopy Sciences) in PHEM fixation buffer (20 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... Thin sections were stained with Uranyless (EMS, Cat. 2240920) and Lead citrate (EMS ...
-
bioRxiv - Cancer Biology 2024Quote: ... and Lead citrate (EMS, Cat. 22410) and imaged using Philips TM10 transmission electron microscope ...
-
bioRxiv - Molecular Biology 2024Quote: Approximately 40 million mammalian cells were crosslinked with 1% Paraformaldehyde (Electron Microscopy Sciences) for 10 min at 37C and quenched with 0.2 M glycine ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were then transferred to a 1% paraformaldehyde (20% PFA, Electron Microscopy Sciences, #15713) in S2 solution and fixed overnight at 4°C while rotating ...
-
bioRxiv - Neuroscience 2024Quote: ... grids were incubated in a solution of 10-nm diameter colloidal gold (EM.BSA 10, Electron Microscopy Sciences, Hatfield, PA, USA) to facilitate subsequent image alignment ...
-
bioRxiv - Physiology 2024Quote: ... After embedding in Durcupan (14040, Electron Microscopy Sciences), ultrathin sections were cut on a Leica Ultra-Microtome ...
-
bioRxiv - Physiology 2024Quote: ... and flat-embedded in Durcupan between liquid release-coated slides (product no. 70880, Electron Microscopy Sciences). After embedding in Durcupan (14040 ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were fixed in 4% paraformaldehyde/PBS (Electron Microscopy Sciences) for 15 min.
-
bioRxiv - Cell Biology 2024Quote: ... and fixed with 4% PFA (EMS, 15710) in PBS for 15 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: 48 hpf Tg(kdrl:eGFP) and Tg(kdrl:Gal4;UAS:RFP;4xNR:dt-Runx1-eGFP) embryos were fixed in 4% formaldehyde (Electron Microscopy Sciences, Cat#15712) diluted in PBS/0.1% tween20 (PBST ...
-
bioRxiv - Cancer Biology 2024Quote: ... then resuspended in 60 µl PBS with 1% BSA and 5 µl of cells was added to each well of a multi-well microscope slide (Electron Microscopy Sciences) pre-coated with 0.1% poly-L-lysine (Sigma Aldrich) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was carefully removed and cells were fixed for 10 minutes at room temperature with 50 µl of 4% paraformaldehyde freshly prepared from a 16% stock (Electron Microscopy Sciences). Cells were again washed with 800 µl PBS with 1% BSA and pelleted ...
-
bioRxiv - Developmental Biology 2024Quote: ... temporal bones were immediately dissected to expose the sensory epithelium and fixed in paraformaldehyde (PFA 4%; Electron Microscopy Sciences; 15710) for 1h at 4°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 4% paraformaldehyde (Electron Microscopy Sciences; 15710) in 1mM MgCl2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... before progressive dehydration in an ethanol series (30-50-70-80-90- 100%, at least 20 minutes per step) and chemical drying with hexamethyldisilazane (HMDS; Electron Microscopy Sciences 50-243-18). Dry samples were mounted on aluminum stubs using double-sided carbon tape and sputter-coated with gold-palladium before imaging on a Hitachi 3000N VP electronic microscope at 20kV.
-
bioRxiv - Developmental Biology 2024Quote: ... and fixed by immersion for at least one overnight at 4°C in 2.5% glutaraldehyde (Electron Microscopy Sciences; 16200) and 4% paraformaldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Neuroscience 2024Quote: ... Two rounds of 100% acetonitrile (30 minutes each) served as a transitional solvent step before proceeding to epoxy resin (EMS Hard Plus). A progressive resin infiltration series (1:2 resin:acetonitrile (e.g ...
-
bioRxiv - Physiology 2024Quote: ... The brain was removed from the skull and a ∼6 mm thick section collected from the optic chiasm to the mamillary bodies using a rat coronal brain matrix (Electron Microscopy Sciences, 69083-C). The hypothalamus was isolated by removing brain matter above the top of the third ventricle and lateral to the optic tract ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... slices were again washed in PBS before mounting on charged slides and cover-slipped using fluoromount (Electron Microscopy Sciences, Hatfield, PA, USA).
-
bioRxiv - Bioengineering 2024Quote: 10 μL of samples with an optimized concentration (1.0∼1.5 x 1010 /100 μL range) in double-filtered PBS solution were deposited onto the TEM grid (Formvar/Carbon 300 Mesh, Copper; #FCF300-CU, Electron Microscopy Sciences) and incubated for 10 min ...
-
bioRxiv - Cell Biology 2024Quote: ... 1.5 round glass coverslips (Electron Microscopy Sciences) and fixed in 4 % w/v paraformaldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Cell Biology 2024Quote: ... and fixed in 4 % w/v paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 min at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... 4% PFA (EMS), and 0.2% glutaraldehyde (EMS) ...
-
bioRxiv - Bioengineering 2024Quote: ... Pellets were cultured for 14 days before being fixed with 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA) overnight at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... mildly crosslinked in 0.2% glutaraldehyde (Electron Microscopy Sciences) for 40 minutes on ice and quenched by addition of 40 µl of 1M Tris pH 7.4 ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, #15710) for 10 minutes at room temperature ...
-
Design and Evaluation of Nanoscale Materials with Programmed Responsivity towards Epigenetic EnzymesbioRxiv - Bioengineering 2024Quote: A drop of nanoparticle sample obtained from the self-assembly of PEG-block-poly (acetylated L-lysine) co-polypeptide was placed on a 300-mesh Formvar carbon-coated copper TEM grid (Electron Microscopy Sciences) for 1 min and wicked off ...
-
bioRxiv - Biochemistry 2024Quote: ... and the cell nuclei were counter stained with 15% Ehrlich’s hematoxylin (Electron Microscopy Sciences). IHC staining of HS were performed similarly except biotinylated rabbit anti-human secondary antibody was used ...
-
bioRxiv - Bioengineering 2024Quote: ... sterile ringer’s solution (Electron Microscopy Sciences) was injected for 72 hours post-surgery to treat dehydration.
-
bioRxiv - Biochemistry 2024Quote: ... Cells were then fixed with 4% paraformaldehyde (Electron Microscopy Science) in PBS for 10 min ...
-
bioRxiv - Biochemistry 2024Quote: ... PKA-treated Ycf1 sample was applied to a QF-1.2/1.3-4Au 400 mesh grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10 °C and 80% humidity ...
-
bioRxiv - Biochemistry 2024Quote: MscS2Δ2-29 was stained with 1.5% uranyl-acetate (EMS), as follows ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
-
bioRxiv - Biochemistry 2024Quote: The specimen was vitrified using 3 μL of freshly purified BasC-Nb71 complex in 0.17% DM applied to glow-discharged Quantifoil R 0.6/1 Cu 300 mesh grids (Electron Microscopy Sciences), at a concentration of 2.2 mg/ml ...
-
bioRxiv - Bioengineering 2024Quote: Two 20 µL droplets of water for injection (WFI) and two 20 µL droplets of negative stain (UranyLess EM stain, Electron Microscopy Sciences) were placed onto parafilm ...
-
bioRxiv - Bioengineering 2024Quote: ... ultrathin copper grids coated with 400 mesh carbon film (FCF400-Cu-UB, Electron Microscopy Science, USA) were used with glow discharge treatment for 1 min before use ...
-
bioRxiv - Bioengineering 2024Quote: ... 3 wt% K2 in Milli-Q water was diluted in Milli-Q water to a final concentration of 0.1 wt% and added to glow discharged Quantifoil CUR 1.2/1.3 400 mesh grids (Electron Microscopy Sciences, Hatfield, PA). Samples were then frozen using a Vitrobot Mark IV Plunge System (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2024Quote: ... and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, #15710) in DPBS for 10 minutes at room temperature ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were fixed in 4% paraformaldehyde (16% PFA, 15710, Electron Microscopy Sciences) in 1x DPBS for 20 min and washed with DPBS four times at 5 min each ...
-
bioRxiv - Bioengineering 2024Quote: ... Conductive coating (Graphite Conductive Adhesive, Electron Microscopy Sciences, USA) was applied at the surface between current collector and carbon felt in order to enhance the electric connection between both materials.28 A cation exchange membrane (CEM ...