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Citations for Electron Microscopy Sciences :
1601 - 1650 of 2438 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: MCN were fixed from 15th to 19th day in vitro (DIV) with 4 % EM grade PFA (Electron Microscopy Sciences, cat. no. 15710) diluted in PEM buffer (80 mM PIPES ...
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Presynaptic mitochondria volume and abundance increase during development of a high-fidelity synapsebioRxiv - Neuroscience 2019Quote: ... Tape containing sections was attached to a 4-inch silicon wafer in rows using double sided carbon tape (EMS, Cat# 77817-50) and sections were post-stained with uranyl acetate and lead citrate ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated 5min in 200mM KCl to stop heart beating and fixed for 2-3 hours in 4% PFA (Electron Microscopy Science, #15710S) at 4°C.
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bioRxiv - Cell Biology 2020Quote: ... the cells were fixed using 4% paraformaldehyde (Serva, Heidelberg, Germany) and 2% glutaraldehyde (Serva) in PBS followed by staining with 0.5% osmium tetroxide (EMS, Hatfield, PA, USA). After thorough rinsing in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 4 µl protein samples was applied to glow discharged Quantifoil R1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA, USA) using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately placed on dry ice for protein or RNA extraction or fixed in fresh 4% paraformaldehyde (PFA, #1570-S, Electron Microscopy Science) for histological study ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed with PBS (not containing Mg2+ and Ca2+) thrice and fixed with 4 % paraformaldehyde (PFA, Electron Microscopy Sciences, 15714) in PBS for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown on glass coverslips with or without collagen (invadopodia formation assay) or gelatin-coated coverslips (degradation assay) and fixed with 4% paraformaldehyde/PBS (Electron Microscopy Sciences). For the invadopodia assay on collagen-coated coverslips ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and equilibrated in 30% sucrose overnight at 4°C before being embedded in Tissue Freezing Medium (Electron Microscopy Sciences) and stored at -80°C ...
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bioRxiv - Bioengineering 2021Quote: ... The tissue-HDMI-array sandwich was moved to room temperature, and the tissues were fixed in 4% formaldehyde (100 μl, diluted from the EM-grade 16% paraformaldehyde (#15170, Electron Microscopy Sciences)) for 10 min.
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bioRxiv - Plant Biology 2020Quote: ... Samples were freeze-substituted in a Leica AFS2 automatic freeze substitution unit at −85°C for 4 days in 2% (w/v) osmium tetroxide (Electron Microscopy Sciences) and 8% 2,2-dimethoxypropane (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... then fixed for 45 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at room temperature for 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Physiology 2022Quote: ... Adult fish were cardiacally perfused with a solution of 4% paraformaldehyde (PFA) diluted in phosphate buffered saline (PBS) solution (Electron Microscopy Sciences) to remove autofluorescence from blood cells40 ...
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bioRxiv - Biophysics 2022Quote: ... Samples of cores undergoing ERT for 4 h at 37 °C were mixed with equal volumes of 10-nm BSA Gold Tracer (Electron Microscopy Sciences), and 3.5 uL aliquots were applied onto glow-discharged Quantifoil grids (Electron Microscopy Sciences) ...
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bioRxiv - Cell Biology 2022Quote: ... falciparum 3D7 culture was pelleted by centrifugation (1500g, 3 min, RT) and resuspended in freshly prepared PBS containing 4% EM-grade para-formaldehyde (PFA, Electron Microscopy Sciences) and 0.1% EM-grade GA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... the unroofed cells were placed in fresh 4% PFA (for immunofluorescence) or in 2% glutaraldehyde (for TEM, EM grade, Electron Microscopy Science) for fixation at 4C.
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bioRxiv - Neuroscience 2021Quote: At the end of experiments mice were perfused with phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science, USA) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were filleted on sylgard plates in room temperature (RT) PBS and fixed on a shaker in 4% paraformaldehyde (Electron Microscopy Sciences, EMS) in PBS for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... infected cells were washed twice with PBS buffer (pH 7.4) and fixed with a 4% methanol-free paraformaldehyde (Electron Microscopy Sciences, 15710) in PBS buffer (pH 7.4 ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... strains were grown in SCD overnight to mid-log phase and fixed with 4% EM grade formaldehyde (Electron Microscopy Sciences 15714-S) for 45 minutes ...
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bioRxiv - Genomics 2020Quote: Protein ICC was performed as described previously (Martin et al., 2013) on cells fixed in 4% paraformaldehyde or 2% paraformaldehyde (Electron Microscopy Sciences). For double and triple immunocytochemistry using antibodies to methylated DNA ...
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bioRxiv - Neuroscience 2019Quote: ... Cat# H-12-1.5-PLL) and fixed for 23 minutes in fresh 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, Cat. 15710) in PBST ...
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bioRxiv - Physiology 2019Quote: Pieces of 0.5 cm2 of whole SC-AT and BM-AT were fixed with a 4% paraformaldehyde solution (PFA, Electron Microscopy Sciences (EMS)) overnight ...
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bioRxiv - Developmental Biology 2020Quote: The selected MC12 cell lines were plated on a 35-mm glass bottom dish (IWAKI) and fixed with 4% formaldehyde (Electron Microscopy Sciences) in 250 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Bioengineering 2020Quote: Both the 3D bioprinted and the manually prepared SK-N-BE(2) neuroblastoma spheroids were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) diluted in 1% Triton (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: Neurons were rinsed 1X with PBS at room temperature and then fixed for 15 minutes at room temperature with 4% paraformaldehyde (Electron Microscopy Sciences) made up in PBS and supplemented with 4% sucrose ...
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bioRxiv - Neuroscience 2020Quote: ... plated in glass bottom dishes or hESC-derived striatal neurons on coverslips were washed in D-PBS and fixed for 10 min in 4% PFA (Electron Microscopy Sciences). The cells were washed 3 times with D-PBS and incubated with DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Microbiology 2021Quote: ... of 0.01 and cultured in 6-well plates for 48 h with cMEM or cells from ETAs of COVID-19-infected patients were fixed with a freshly prepared 2% or 4% PFA solution (20% stock diluted in 1X HBSS; Electron Microscopy Sciences) or a 1.6X BD FACS™ Lysis Solution (1:6 in sterile dH2O ...
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bioRxiv - Physiology 2021Quote: ... whole heads or enucleated eyes were fixed for 20 minutes on ice in 4% PFA prepared in PBS from 20% stock solution (15713, Electron Microscopy Sciences). The samples were rinsed with PBS and stored in 100% methanol at −20°C ...
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bioRxiv - Neuroscience 2019Quote: ... Cat# H-12-1.5-PLL) and fixed for 23 minutes in fresh 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, Cat. 15710) in PBST ...
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bioRxiv - Immunology 2020Quote: ... Naive T cells were maintained 4 days in IL-7-supplemented medium and then fixed overnight in 2.5% glutaraldehyde (Electron Microscopy Sciences, UK) at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2021Quote: ... the entire liquid medium was gently discarded from each dish and the adherent cells were immediately fixed with 4% PFA (Electron Microscopy Sciences), 0.1% glutaraldehyde (G5882 ...
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bioRxiv - Neuroscience 2021Quote: ... the patch pipette was gently removed from the recorded PC and the slice was immediately transferred from the recording chamber to a fixation solution composed of 4% paraformaldehyde (Electron Microscopy Sciences) in ACSF for a maximum of 24h (4°C).
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then fixed for 10 minutes at room temperature in PBSM supplemented with 4% paraformaldehyde (Electron Microscopy Sciences, 50-980-492). Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were incubated with 200nM Mitotracker for 30 min before fixing them in 4% paraformaldehyde (Electron Microscopy Sciences Cat# 15710, Hatfield, PA) for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... all animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The animals were then transcardially perfused with PBS and then with 4% paraformaldehyde (PFA) (diluted into 1xPBS from 16% PFA, Electron Microscopy Sciences), followed by post-fixation with 4% PFA for immunohistochemistry or Karlsson and Schultz (KS ...
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bioRxiv - Neuroscience 2024Quote: Mice were placed under isoflurane anesthetics until loss of pain reflex and transcardially perfused with filtered ice-cold PBS then 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were cryoprotected in 30% sucrose ...
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bioRxiv - Neuroscience 2024Quote: ... mice were perfused with phosphate-buffered saline (PBS, warmed up to 37 °C) with heparin solution (20 units / mL) followed by 4% paraformaldehyde (PFA, Electron Microscopy Science) in PBS ...
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bioRxiv - Neuroscience 2024Quote: P14 mice were euthanized and perfused transcardially with ice-cold Phosphate Buffered Saline (PBS) followed by ice-cold 4% paraformaldehyde (Electron Microscopy Sciences) diluted in water ...
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bioRxiv - Neuroscience 2024Quote: Mice were deeply anesthetized with a mixture of Ketamine/Xylazine and perfused transcardially with saline followed by 4% paraformaldehyde (PFA; Electron Microscopy Science). Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed three times with pre-warmed phosphate-buffered saline (PBS) before being fixed in 4% paraformaldehyde (Electron Microscopy Sciences #15710) in PBS for 15 mins at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was carefully removed and cells were fixed for 10 minutes at room temperature with 50 µl of 4% paraformaldehyde freshly prepared from a 16% stock (Electron Microscopy Sciences). Cells were again washed with 800 µl PBS with 1% BSA and pelleted ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown on 35-mm Glass Based Dishes which have a cover glass with a grid pattern (IWAKI) and fixed after laser-microirradiation with 4% PFA (Electron Microscopy Sciences) containing 0.1% Triton X-100 (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibody incubation was followed by 4 x 15-minute washes in 1X PBS and slide mounting using Fluor-Gel mounting medium (Electron Microscopy Sciences). Tissues were mounted epithelium-side up.
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bioRxiv - Biochemistry 2024Quote: To generate negative-stain grids 4 µl of the GraFix fraction was applied to a Copper 400 Mesh grids with continuous carbon (Electron Microscopy Sciences) after glow discharge ...
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bioRxiv - Immunology 2023Quote: FACS-sorted naïve CD4+ T cells from LNs and spleen were pelleted at 400 g for 4 mins by swinging bucket Eppendorf 5810 into BEEM conical capsule tips (EMS, #69913-05), and fixed in 2% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (CB ...