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Citations for Electron Microscopy Sciences :
1451 - 1500 of 2438 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Cells were harvested by centrifugation and fixed at room temperature while nutating in 4% paraformaldehyde (Electron Microscopy Sciences #15710). Cells were washed with PBS with 2mM EDTA and permeabilized at 45°C with 2% w/v sodium lauroyl sarcosine (Bioworld #41930024-3 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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bioRxiv - Cell Biology 2023Quote: Embryonic tissues for cryosections were fixed for 2h in 4% paraformaldehyde (PFA) in PBS (Electron Microscopy Sciences, Cat #:15710) with 0.5% Triton X-100 at 4°C ...
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bioRxiv - Genetics 2023Quote: ... coverslips were washed with PBS and fixed at RT for 10 minutes with 4% PFA (Electron Microscopy Sciences, 15713S). After fixation ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended either in 0.3 mL Milli-Q water for vitrification or 4 % para-formaldehyde (EM grade #15710 from EMS) in 1x PBS for chemical fixation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells on transwell filters in well plates were fixed with 4% paraformaldehyde (#15710, Electron Microscopy Sciences, Hatfield, PA, USA) in PBS for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Developmental Biology 2023Quote: ... all WMISH fixations were done in 4% paraformaldehyde (PFA, stock 32% PFA ampules from Electron Microscopy Sciences, cat. 15714) in ASW for 6 h–overnight at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... were imbibed overnight in water at 4 °C and then incubated for 12 h in 50 mL of 0.25% ethyl methane sulfonate (EMS). The EMS was subsequently washed several times with water ...
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bioRxiv - Cell Biology 2024Quote: ... The tissue blocks were rinsed three times with water and en bloc stained overnight at 4°C with an aqueous solution of 0.5% w/v uranyl acetate (EMS). The tissue blocks were dehydrated in ethanol ...
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bioRxiv - Bioengineering 2024Quote: ... cells were fixed for 30 minutes at room temperature with 4% paraformaldehyde (Electron Microscopy Science, Hatfield, PA, USA; 15700) in PBS and then permeabilized with 90% methanol for 10 minutes at –30°C ...
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bioRxiv - Genetics 2024Quote: ... Brains were then fixed in 0.1% PBST (0.1% Triton-X-100 in 1X PBS) containing 4% paraformaldehyde (EMS Biosciences) for 20 minutes with rocking at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed three times with PBS and then fixed in 4% formaldehyde (Electron Microscopy Sciences, Cat. 15714) for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... and vacuum infiltrated with fixative solution composed of 4% glutaraldehyde (v/v, EM Grade, Electron Microscopy Sciences, Cat# 16020) and 4% formaldehyde (v/v ...
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bioRxiv - Neuroscience 2024Quote: Mice were perfused with 20mL of Phosphate Buffered-Saline followed by 20 mL 4% paraformaldehyde (PFA, Electron Microscopy Sciences). The brains were stored in 4% PFA overnight and washed 3x5 min with PBS the next day ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were dissected in IMADS (Singleton and Woodruff 1994) or 1X PBS and fixed with 4% PFA (Electron Microscopy Sciences) in 1X PBS plus 0.3% Triton-X 100 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were first washed with PBS for 5 minutes and then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, PA, USA) in PBS containing 0.1% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... samples were washed once in wash buffer and fixed with a 4% Paraformaldehyde + 0.1% Glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) solution made in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected larvae were fixed for 20 (VNC) or 45 minutes (NMJs) in 4% formaldehyde solution (Electron Microscopy Sciences, Hatfield, PA) in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... When animals reached the indicated age they were anaesthetized with isoflurane and intracardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences) in PBS was performed ...
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bioRxiv - Immunology 2019Quote: ... The right-side half brains were harvested and fixed in 4% paraformaldehyde (made in PBS; #15710, Electron Microscopy Sciences, USA) at 4◻ for 6 days ...
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bioRxiv - Biochemistry 2020Quote: ... Cell were then fixed for 10 minutes at room temperature in PBSM + 4% paraformaldehyde (Electron Microscopy Sciences 50-980-492). Following fixation ...
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bioRxiv - Neuroscience 2020Quote: ... perfused intracardially with ice-cold phosphate-buffered saline (PBS) and fixed with 4 % paraformaldehyde (PFA, 19210, Electron Microscopy Sciences, USA) in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2020Quote: Adult mice were anesthetized with isoflurane and transcardial perfusion was performed with 7.5 ml of 1x PBS following by 7.5 ml of ice cold 4% PFA in 1x PBS (EMS – 15710-S). Brains were then dissected out and post-fixed in 4% PFA in 1x PBS for 2 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Post infection coverslips were rinsed in PBS then fixed for 10 minutes in 4% Paraformaldehyde in PBS (Electron Microscopy Sciences) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of parasite culture at 5% parasitemia was washed with PBS and re-suspended in a fresh fixative solution (4% Paraformaldehyde (EMS) and 0.0075% glutaraldehyde (EMS ...
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bioRxiv - Cell Biology 2022Quote: ... and lens were gently removed and the resulting posterior eyecup was fixed in freshly prepared ice-cold 4% (w/v) PFA (171010, EMS) in PBS for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused transcardially with heparinized saline (10 units/ml of heparin) and 4% paraformaldehyde (PFA) that was prepared from 32% PFA Aqueous Solution (Cat# 15714, EMS), using a peristaltic pump at a rate of 7 mL/min ...
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bioRxiv - Biochemistry 2022Quote: ... followed by application of 4 μl of the mix onto glow discharged continuous carbon electron microscopy grid (400 mesh, EMS) and was incubated on grid for 1 min ...
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bioRxiv - Biochemistry 2022Quote: ... and then 4 μl of the mixture was added to a Quantifoil R1.2/1.3 Au 300 mesh grid (Electron Microscopy Sciences) that had been glow discharged for 60 seconds at 20 mA using a Pelco easiGlow ...
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bioRxiv - Bioengineering 2022Quote: ... the media is removed from the wells and the cells are fixated via a 4% paraformaldehyde (PFA, Electron Microscopy Sciences) solution incubation for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... to a final concentration of 0.005 mM and 4 μL of this solution was applied to plasma-cleaned 1.2/1.3 C-Flat holey carbon grids (Electron Microscopy Sciences) using a Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Fixation buffer consisting of 4% paraformaldehyde (Alfa Aeser, USA, CAT#:43368) and 0.1% glutaraldehyde (Electron Microscopy Sciences, USA, CAT#: 16100) was used to fix cells on the coated surfaces.
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bioRxiv - Neuroscience 2022Quote: ... the recipient mice eyes and cultured retinal organoids were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) in PBS and dehydrated in a sucrose gradient (10% ...
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bioRxiv - Neuroscience 2022Quote: ... animals were transcardially perfused with ice cold 1x PBS followed by ice cold 4% PFA (Electron Microscopy Sciences, 100504-858). Spinal cords were dissected and post-fixed in 4% PFA for 2 hours at 4°C ...
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bioRxiv - Bioengineering 2019Quote: Smooth muscle cells (A10 and MOVAS) were fixed in methanol-free 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, cat. no. 15710) for 10 mins diluted in PBS++ (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... the 3D printed constructs were kept in culture for 3 weeks before fixing using paraformaldehyde (4%, Electron Microscopy Sciences, USA) for 45 minutes at RT ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed with 1X PBS and fixed in 4% paraformaldehyde (PFA) solution (diluted from 16% PFA, Electron Microscopy Sciences) in PBS for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with ketamine/xylazine before transventricular cardiac perfusion with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA; Cat #19210) and 2.5% glutaraldehyde (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... At 10.5 dpc the mice were sacrificed by cervical dislocation and embryos were fixed for 3 hours in 4% paraformaldehyde (PFA, EMS). Immunofluorescence on sections was performed as follows ...
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bioRxiv - Immunology 2019Quote: ... 10cm long) were gently swiss rolled from the distal end and fixed in 4% paraformaldehyde (Electron Microscopy Science, Hatfield USA). Formalin-fixed tissues were then processed for paraffin embedding ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μL solution in each reaction condition was immediately transferred to a glow-discharged continuous carbon grid (Electron Microscopy Sciences) for 15 seconds before blotting and staining ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with 0.9% NaCl followed by a mixture of 4% PFA and 0.1% glutaraldehyde (Electron Microscopy Sciences) in 0.1M phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2020Quote: ... we immersed slides from −80 °C instantly into freshly made 4 % PFA (10mL vials of 20 % PFA; Electron Microscopy Sciences) in PBS for 30 mins at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Microtissues were transferred to wells with 4% PFA in PBS (diluted from 32% PFA solution, Electron Microscopy Sciences 15714-S) for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... EVs were fixed by positioning the grid on top of a 20 µL drop of 2% paraformaldehyde (EMS, 157-4) for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Cas9 expression was confirmed by immunofluorescence with paraformaldehyde (4% f.c, 15 min, room temperature, Electron Microscopy Sciences, Cat. #15713-S) fixed cells ...
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bioRxiv - Neuroscience 2023Quote: E16.5 and E18.5 embryos were sacrificed by head sectioning and brains were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) in Phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Several FA/GA-fix tissue sections were post-fixed with 0.01% osmium tetroxide (4% osmium solution; EMS, catalog no. 19150) in 1× PBS for 1h at RT (FA/GA/OsO4-fix) ...
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bioRxiv - Neuroscience 2022Quote: Anesthetized mice were transcardially perfused with ice cold phosphate buffered saline (PBS) followed by 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Brains were post-fixed in 4% PFA overnight and cryoprotected in 30% Sucrose (Sigma-Aldrich) ...