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Citations for Electron Microscopy Sciences :
1751 - 1800 of 2438 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... were injected intraperitoneally in n=3 control and n=3 KA Nestin-GFP animals 30 minutes before perfusion with a PFA 4 %-Glutaraldehyde 0.5 % (Cat#16536-05, Electron Microscopy Sciences, Hatfield, PA, USA) mixture ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with ketamine/xylazine and transcardially perfused with PBS followed by PFA (4% in PBS; Electron Microscopy Sciences, cat n°15700). Extracted brains were post-fixed (PFA 4% overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and pancreas were harvested at the experimental endpoint and immediately fixed in 4% paraformaldehyde in 1X PBS (16% solution, Electron Microscopy Sciences, cat# 15711) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Transcardiac perfusion was done with a prewash with 9.25% sucrose in distilled water and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion TwoTM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... rats were transcardially perfused with an isotonic solution at 9.25% in distilled water as a prewash and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Biophysics 2023Quote: ... Grids were made using a Vitrobot™ Mark IV (FEI) at 4 °C and 100 % humidity with Grade 595 Vitrobot™ filter paper (Electron Microscopy Sciences). All grids were plunge frozen using liquid ethane and stored in liquid nitrogen.
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bioRxiv - Developmental Biology 2023Quote: Gels were fixed by 30 minutes rotation incubation at room temperature in freshly prepared 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA, Cat# 15710S) diluted in PBS-T ...
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bioRxiv - Developmental Biology 2023Quote: ... or any of the four possible distributions at the 4-cell stage (ABa and P2; ABa and EMS; ABp and P2; ABp and EMS), were more likely to harbor sperm-contributed centrioles using the χ2 test as implemented in the library SciPy ([https://scipy.org/citing-scipy/]) ...
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bioRxiv - Neuroscience 2024Quote: ... animals were anesthetized in 0.02% MS-222 and then immersed in 4% PFA (diluted from 16% PFA in 1xPBS, Cat # 15710, Electron Microscopy Sciences, Fort Washington, PA) using a Pelco Biowave Pro microwave (Model 36700 ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and then perfused transcardially with room temperature (RT) phosphate buffered saline (PBS) with heparin (0.02 mg/mL) and then ice-cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, catalog no: 15714). Brains were dissected and post-fixed in 4% PFA overnight at 4°C and cryopreserved in 30% sucrose in PBS for ∼2 days at 4°C ...
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bioRxiv - Genetics 2021Quote: We screened for lst-1 or sygl-1 enhancers using a modified ethyl methanesulfonate (EMS) protocol (Brenner 1974) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were fixed at 14-DIV for 1-h in 1% glutaraldehyde (EMS, Hatfield, USA) diluted in 0.1M cacodylate buffer ...
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bioRxiv - Molecular Biology 2020Quote: 1×105 of U2OS-EpiGo cells were cultured on the #1 coverslip (Electron Microscopy Sciences) and added 500 ng/ml doxycycline for induction of dCas9 or dCas9-KRAB expression ...
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bioRxiv - Immunology 2021Quote: ... tissues were incubated in a 1:1 mixture of EMBed 812 (Electron Microscopy Sciences 14120) and propylene oxide ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed for 1 hour at room temperature with 1% OsO4 (Electron Microscopy Science, 19100), dehydrated in the ethanol/acetone series and embedded in Epon/Araldite epoxy embedding media (Electron Microscopy Science ...
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bioRxiv - Neuroscience 2021Quote: ... Brains from adult females were dissected in Phosphate-buffered saline (PBS, Eurobio, ref CS0PBS0108) and fixed for 15 min in 4% paraformaldehyde (Electron Microscopy Sciences, Cat # 15714-S) at room temperature (RT) ...
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bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
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bioRxiv - Genomics 2020Quote: Ten million cells from two MSC preparations at early (P2 and 3) and late (P7 and 9) passages were cross-linked with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 10 min. ...
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bioRxiv - Developmental Biology 2021Quote: ... The eye imaginal discs were dissected in cold 1X solution of Phosphate Buffered Saline (PBS) and fixed using 4% p-formaldehyde (Electron Microscopy Sciences, Catalog no. 15710). Fixed tissues were washed using PBS-T [0.2% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: Immediately after cold exposure mice were anaesthetized with isoflurane (3%) and perfused with heparinized saline followed by 4% paraformaldefyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA). Fat pads were carefully dissected and postfixed overnight in 4% PFA at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... the cultured neurons were fixed for 10-15 min by transferal of the coverslip into a dish with 4% paraformaldehyde (16% aqueous solution, 15710; Electron Microscopy Sciences, Hatfield, Pennsylvania, USA) and 0.2% glutaraldehyde (25% aqueous solution ...
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bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
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bioRxiv - Genomics 2020Quote: ... cells were fixed by adding the fixative directly to the medium to a final concentration of 4% paraformaldehyde (PFA) (Electron Microscopy Sciences; cat. no. 15714) using the automated liquid handler BlueWasher (BLUE CAT BIO) ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... mice were placed under anesthesia and tissues were perfused with 10mL of cold PBS and 5mL of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, #15710, Hatfield, PA). Brains were post-fixed overnight in 4% PFA at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... we soaked the dehydrated sample in 1:1 ethanol:HMDS (hexamethyldisilazane, 16700, Electron Microscopy Sciences, Inc., PA), followed by pure HMDS ...
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bioRxiv - Molecular Biology 2023Quote: Carbon-coated holey grids Quantifoil R 0.6/1 Cu 300 and CF-2/1-3C (EMS) were used for preparing cryo-EM samples for apo GSEC1B and GSEC1B-Aβ46 complex ...
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ITPK1 is an InsP6/ADP phosphotransferase that controls systemic phosphate homeostasis in ArabidopsisbioRxiv - Plant Biology 2020Quote: ... pho2-1 (EMS mutant described previously43) and phr1 (SALK_067629 ...
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bioRxiv - Cell Biology 2019Quote: ... 1% glutaraldehyde (Electron Microscopy Sciences, 16000), or a combination of 1% glutaraldehyde and 4% paraformaldehyde in PBS ...
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bioRxiv - Microbiology 2021Quote: ... and in osmium tetroxide 1% (EMS) with 1.5% of potassium ferrocyanide (Sigma ...
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bioRxiv - Immunology 2019Quote: ... stained in 1% osmium tetroxide (EMS) in cacodylate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and (ii) 1% osmium (EMS, 19134) plus 0.2% uranyl acetate (20% stock in methanol ...
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bioRxiv - Cell Biology 2019Quote: ... post-fixed with 1% OsO4 (EMS) and 1% tannic acid (EMS ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% paraformaldehyde (PFA) (Electron Microscopy Sciences) was used for cell cross-linking ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% paraformaldehyde (Electron Microscopy Sciences). Cells were then washed in 1x TBS and blocked in antibody dilution buffer (Abdil ...
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bioRxiv - Cell Biology 2021Quote: ... with 1% paraformaldehyde (Electron Microscopy Sciences) for 10 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in 1% PFA (EMS). The cells were incubated for 2 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... containing fixative (1% glutaraldehyde (EMS 16530), 0.1% Tannic acid (Sigma 403040-100G ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% osmium tetroxide (EMS, Cat# 19110), and 1.5% potassium ferrocyanide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 1% lead citrate (EMS, Cat# 17800), 1% lead nitrate (EMS ...
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bioRxiv - Cell Biology 2023Quote: ... 1% lead nitrate (EMS, Cat# 17900), 2% sodium citrate diluted (EMS ...
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bioRxiv - Neuroscience 2019Quote: ... Heads bearing both eyestalks were removed and placed immediately in freshly made ice-cold fixative containing 4% paraformaldehyde (PFA; Electron Microscopy Sciences; Cat# 15710; Hatfield, PA) and 10% sucrose in 0.1 M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The slice was then left to rest in the recording chamber for 4-5 minutes to ensure transport of biocytin to distal dendrites and axon processes (Swietek et al., 2016)39 before the slice was moved into 4% PFA (Electron Microscopy Sciences, CAS #50-00-0) diluted in 0.1M Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... coli BioParticles™ conjugate or DQ™ Green BSA was added for 30min at 4°C and moved to Cytoseal mounting medium (Electron Microscopy Sciences, Hatfield, PA) set at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... then samples were embedded for 2 h with 1:1 acetone and epon resin (Embed-812, EMS), followed by 100% epon resin at 60°C for 2 days ...
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bioRxiv - Zoology 2021Quote: ... and post-fixed for 1 hour at room temperature (RT) with 1% OsO4 (Electron Microscopy Science, 19100). Fixation and post-fixation were done in modified 0.1M Na-Cacodylate buffer (0.1M Na-Cacodylate ...
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bioRxiv - Neuroscience 2023Quote: ... The pure acetone was replaced with a 1:1 mixture of acetone and Embed 812 resin (EMS), and the samples were allowed to infiltrate overnight on a rotating shaker ...