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Citations for Electron Microscopy Sciences :
1001 - 1050 of 1075 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with freshly prepared and filtered 1% tannic acid (TA, CAS#1401-55-4, EMS) in water using a Pelco BioWave microwave for seven 1-minute cycles alternating between 150 W and 0 W power ...
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bioRxiv - Developmental Biology 2022Quote: ... and submerged in 200°C heated R-Universal Epitope Recovery Buffer solution (Electron Microscopy Sciences, Hatfield, PA, 62719-20) for 30 minutes and then let cool at room temperature for 25 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of 4ul were applied on glow-discharged UltraAuFoil R(1.2/1.3) 300 mesh gold grids (Electron Microscopy Sciences). The grids were then blotted with filter paper and plunge-frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed in D-PBS and treated with Sodium Citrate based-R-buffer A (EMS, #62706-10) in 2100-Retriever (EMS ...
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bioRxiv - Biophysics 2023Quote: ... MMA/graphene was applied onto 300 mesh gold-coated Quantifoil R 0.6/1 copper grids (Electron Microscopy Sciences, USA) by using the grids to scoop out the bilayer ...
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bioRxiv - Bioengineering 2023Quote: ... followed by pre-treatment with 6% nitric acid and sequential staining with 0.1% toluidine blue (EMS #26074-15) and picrosirius red (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then freeze substituted at - 90°C with 0.1% (w/v) uranyl acetate and 0.01% (w/v) tannic acid (EMS) in acetone for 6h using a Leica EM AFS2 with a processor Leica EM FSP ...
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bioRxiv - Biophysics 2021Quote: ... 3 ul of this mixture was applied to glow-discharged 400 mesh Cu Quantifoil R 1.2/1.3 grids (Electron microscopy sciences) and plunge frozen in liquid ethane ...
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bioRxiv - Microbiology 2020Quote: ... The mixture (2.5 μL) was applied to freshly glow-discharged (30 s) Quantifoil R 3.5/1 grids (Electron Microscopy Sciences) and manually blotted before being flash-frozen in liquid ethane ...
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bioRxiv - Biochemistry 2024Quote: The specimen was vitrified using 3 μL of freshly purified BasC-Nb71 complex in 0.17% DM applied to glow-discharged Quantifoil R 0.6/1 Cu 300 mesh grids (Electron Microscopy Sciences), at a concentration of 2.2 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then immediately applied to glow-discharged Quantifoil grids (R 1.2/1.3, 400 mesh, copper grids, EMS Cat #: Q450CR1.3) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: All samples for cryo-EM were applied to glow discharged Quantifoil R 1.2/1.3 grids (Au 400; Electron Microscopy Sciences) and plunge frozen in liquid ethane using a Vitrobot Mark IV (FEI Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and mounted on a poly-L-lysine coated coverslip using Fluoromont-G T (Electron Microscopy Science, USA). Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Seeds were collected from a single Columbia founder and treated with 20 μmol/L ethylmethane sulfonate (EMS) for 12 hrs ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in cacodylate buffer containing 1% low molecular weight tannic acid for 30 min (Electron Microscopy Sciences) between the osmication step and the uranyl acetate step ...
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bioRxiv - Pathology 2024Quote: ... Slides were washed with distilled water and stained with Ponceau acid Fuchsin-solution (Electron Microscopy Sciences, Hatfield, PA, USA) for 10 - 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... A small longitudinal incision was then made using a diamond dissecting blade (Type M-DL 72029 L; EMS) along the glue line either between two pharyngeal bulbs for AVL recording or in the tail region for DVB recording ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown on poly-L-lysine coated coverslips and fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) for 10 min at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: TSCs and TGCs were grown on poly-L-lysine–coated #1.5 glass coverslips (Electron Microscopy Sciences, Hatfield, PA) for confocal microscopy ...
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bioRxiv - Bioengineering 2022Quote: ... followed by approximately 1 L of 4% (w/v) paraformaldehyde (PFA) fixative (19208, Electron Microscopy Sciences, Hatfield, PA) in 1X PBS.
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bioRxiv - Developmental Biology 2023Quote: ... was used to counterstain sections and coverslips were mounted using Fluorogel l (Electron Microscopy Sciences, Hatfield, PA, USA). Confocal Microscopy image acquisition was taken on a LSM 710 microscope (Zeiss ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mg/mL tannic acid and 0.5 mg/mL saponin in HKB) for 40 min on a thermanox plastic coverslip (EMS, USA), followed by washing in HKB buffer and then water ...
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bioRxiv - Microbiology 2020Quote: ... and stained at room temperature for 1 hour with 1% (wt/vol) low-molecular weight tannic acid (Electron Microscopy Science) in 01 ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Developmental Biology 2022Quote: ... deparaffinized and antigen retrieval was performed in a Retriever 2100 instrument with R-Universal epitope recovery buffer (Electron Microscopy Sciences, Hatfield, PA). After retrieval ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu, Electron Microscopy Sciences) followed by plunging into liquid ethane using an FEI Vitrobot Mark IV set to a blot time of 22 and a blot force of 22 for QYNNENNFV and 24 for GSTVYAPFT ...
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bioRxiv - Neuroscience 2024Quote: ... followed by heat induced antigen retrieval in a programmable antigen retrieval cooker (Aptum Bio Re-triever 2100) using 1X R-UNIVERSAL Epitope Recovery Buffer (EMS, #62719-10). The sections were then blocked with BLOXALL® Endogenous Alkaline Phosphatase Blocking Solution (VectorLabs ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then plated as attached OPCs on 0.01% Poly-L-Lysine coated plates (Electron Microscopy Sciences, 19320-B) in the same media ...
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bioRxiv - Cell Biology 2021Quote: ... donkey anti-mouse IgG (H&L) conjugated with 15-nm gold particles (Electron Microscopy Science Aurion #25817, PA, USA).
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bioRxiv - Bioengineering 2022Quote: ... the first microfluidic device was placed on a clean poly-L-lysine coated slide (PLL slide, Electron Microscopy Science) and baked for 2 hours at 75°C oven to strengthen the bonding ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μL of the collected hemolymph was applied to poly-L-lysine slides (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to rest for ten minutes at room temperature on a flat surface (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells plated on glass bottom dishes and platelets immobilized on poly-L-lysine (Electron Microscopy Sciences, Hatfield, PA)-coated on glass bottom dishes were fixed with 4.0 % paraformaldehyde (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 500 μM nucleic acid intercalator iridium (191Ir and 193Ir, Fluidigm) in PBS with 1.6% PFA (Electron Microscopy Sciences) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and the tissue sectioned at 70nm using a Leica Microsystems Ultracut R ultramicrotome (Wetzlar, Germany) outfitted with a Diatome Ultra 45° diamond knife (EMS-Diatome, Hatfield, PA). Sections ...
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bioRxiv - Pathology 2024Quote: ... then incubated overnight at room temperature in LFB solution (0.1% in 95% alcohol with acetic acid, Electron Microscopy Sciences, Hatfield, PA, USA). In groups of 3 or 4 with at least one normal sample ...
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bioRxiv - Cell Biology 2024Quote: ... The vibratome sections were stained with 1% tannic acid and 1% uranyl acetate (21700 and 22400, Electron Microscopy Sciences, Hatfield PA USA), dehydrated with ethanol ...
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bioRxiv - Immunology 2021Quote: ... A 4-μl drop of the diluted sample was applied to a freshly glow-discharged carbon-coated copper grid (400-mesh, EMS) for approximately one minute ...
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bioRxiv - Bioengineering 2023Quote: 5 μL of D-GQDs/exo loaded on the Poly-L-Lysine coated slides (63410; Electron Microscopy Sciences, PA, USA) and was fully covered with 18 mm × 18 mm square cover glasses (2845-18 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 mg/ml 70S ribosome RafH complex was applied on a glow discharged 300 CF300-Cu grids (EMS), the excess sample was blotted ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 µL of protein sample were applied to freshly glow discharged Quantifoil R1.2/1.3 Cu 200 mesh grids (EMS, Q225-CR1.3) and were frozen by plunging into liquid ethane using an FEI Vitrobot Mark IV (100% humidity ...
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bioRxiv - Neuroscience 2024Quote: ... coronal cryosections of 10 μm were prepared on poly-L-lysine-coated glass slides (no. 63478-AS, Electron Microscopy Sciences) or 50×75 mm poly-L-lysine-coated glass slides ...
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bioRxiv - Biophysics 2024Quote: ... by applying 3 μl of purified XPR1 to a glow-discharged holy carbon gold QUANTIFOIL 1.2/1.3 grid (400 mesh, Electron Microscopy Sciences) and blotting for 4 to 5 s at 4°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... We then immediately applied the mixture to glow-discharged Quantifoil grids (R 0.6/1, 300 mesh, gold, Electron Microscopy Sciences [EMS] Cat #: Q350AR-06) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... We then immediately applied the mixture to glow-discharged Quantifoil grids (R 0.6/1, 300 mesh, gold, Electron Microscopy Sciences [EMS] Cat #: Q350AR-06) and blotted once for 6 s after a wait time of 30 s in 100% humidity at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ∼5-6μl of the resuspended 100,000 ×g pellet fraction was spread onto glow discharged Formvar-coated copper mesh grids (Electron Microscopy Sciences) and stained with 2% Uranyl acetate for 2 min ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
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bioRxiv - Microbiology 2020Quote: ... purified EVs were resuspended in 30 μL D-MEM (0% FCS) and mixed with a low volume of Glutaraldehyde 25% (final concentration = 2.5%) (16220, Electron Microscopy Sciences). After 10 min of fixation at RT ...
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bioRxiv - Cell Biology 2022Quote: ... For immunofluorescence analysis cells were seeded (1.5 x 105 HeLa cells) directly on poly-L-lysine coated coverslips (Electron Microscopy Sciences), which were placed in a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: A drop (1-2 μl) of undiluted EVs (PEG/SEC-enriched EVs from 100 uninjured egfp(RNAi) animals) was placed on a formvar (EMS 15810)-coated hexagonal grid (EMS G300-Cu ...
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bioRxiv - Biophysics 2023Quote: Four μL of 5 μM GLS2 were applied to plasma-cleaned grids (300 mesh UltraAu Foil R1.2/1.3, Electron Microscopy Sciences, EMS) for a 30-second incubation ...