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Citations for Electron Microscopy Sciences :
701 - 750 of 1075 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and then perfused with RPMI media containing 5 U/mL heparin (Sagent Pharmaceuticals, Cat. #NDC 25021-400-10) followed by 3% paraformaldehyde (PFA) (Electron Microscopy Science, Cat. # 15714-S) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... Grids with the collected monolayers facing up were stained at once with 2% uranyl formate (Electron Microscopy Sciences) in water by depositing 4 μL on the grid and simultaneously blotting the excess solution ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Neuroscience 2021Quote: ... followed by fixation with 2% PFA (w/v; prepared from a 20% stock solution, Electron Microscopy Sciences: 15713) in cell culture medium for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... following which they were placed in a mixture (500 μL) of 2% OsO4 (Electron Microscopy Sciences, cat#19150) and 0.1 M sodium cacodylate buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then postfixed for 1 h on ice with 2 mL of 1% osmium tetroxide (EMS #RT19134) and 1% potassium ferrocyanide (Sigma-Aldrich #P3289 ...
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bioRxiv - Biophysics 2020Quote: ... and permeabilized for 2 min with 0.3 % glutaraldehyde (GA) + 0.25 % Triton X-100 (EMS, 16220 and ThermoFisher, 28314) in pre-warmed (37° C ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 µL of particle solution (20 mg/mL) was applied on a CF400-CU grid (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were fixed in 2% PFA/2.5% glutaraldehyde/0.1 M Sodium Cacodylate Buffer pH 7.4 (Electron Microscopy Sciences). Lateral ventricle walls were fixed for 18 h at 4 °C othen subdissected into anterior ...
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bioRxiv - Neuroscience 2020Quote: ... unbuffered OsO4 aqueous solution for 2 hours and lead aspartate solution (0.03 M, pH 5.0 adjusted by KOH, EMS) at 50 °C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were intracardially perfused with NaCl 0.9% followed by PFA 2% (Electron Microscopy Sciences 15713, diluted in PBS). Brains were post-fixed in PFA 2% and cryoprotected in 20% sucrose overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were stained by a 2 minute bath in 0.1% cresyl violet acetate (Electron Microscopy Sciences #26089-01), followed by a 2 minute rinse in distilled water ...
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bioRxiv - Microbiology 2020Quote: ... and stained with 2% uranyl formate (pH 5.5– 6.0) on thin carbon-layered 400 mesh copper grids (EMS)46 ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos prepared for immunostaining were fixed overnight at 4°C in 2% PFA (Electron Microscopy Sciences, Catalog #15710). Embryos were either embedded in 4% low-melt agarose/PBS + 0.1% Tween and cut into 200um sections using a Leica VT1200 or stained as intact whole-mounts ...
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bioRxiv - Physiology 2023Quote: ... Ovaries used for MIMS were cut in half and fixed in 2% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA) and embedded in LR white (EMS) ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with pentobarbital and perfused with 2% paraformaldehyde (PFA, Paraformaldehyde Granular, Electron Microscopy Sciences, Hatfield, PA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... (2) fixed with 1.5% paraformaldehyde and 1.5% glutaraldehyde in 0.1M sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA, USA), pH 7.3 for 1 h at room temperature followed by 3 washes with 0.1M sodium cacodylate ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 17985-50; Electron Microscopy Sciences, Hatfield, PA, USA). Images were taken with an All-in-One Fluorescence Microscope BZ-X710 (KEYENCE Corp. ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed in freshly diluted 2% paraformaldehyde (PFA) in PBS (16% PFA purchased from EMS Cat# 15710) for 20 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2024Quote: ... 10 μL of parasite-containing media from each flask was added to 90 μL of 2% PFA (EMS) to fix and dilute the parasites at a 1:10 ratio in a 96-well flat bottom plate ...
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bioRxiv - Immunology 2024Quote: ... coated grids (R2/2, Au 200-mesh London Finder grid coated with extra thick carbon, Electron Microscopy Sciences) for another 24 hours to allow sufficient adherence ...
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bioRxiv - Immunology 2024Quote: ... 2% PFA was prepared from 16% PFA solution (Paraformaldehyde 16% Solution, EM Grade, Electron Microscopy Sciences, Hatfield, PA) in nanopure water ...
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bioRxiv - Cell Biology 2024Quote: ... 2% PFA was prepared from 16% PFA solution (Paraformaldehyde 16% Solution, EM Grade, Electron Microscopy Sciences, Hatfield, PA) in nanopure water ...
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bioRxiv - Neuroscience 2022Quote: ... was performed over the vS1 of the mouse and covered with a double-layered round glass coverslip (3 mm: 64-0720 [CS-3R], Warner Instruments, Hamden, CT; 5 mm: Electron Microscopy Sciences, Hatfield, PA, 72195-05), bonded with an optical adhesive (NOA71 ...
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bioRxiv - Developmental Biology 2021Quote: ... whole-mount preparations of the lateral wall of lateral ventricles of four animals per group were dissected and fixed with 2 % PFA + 2.5 % glutaraldehyde (EMS) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Pathology 2022Quote: ... perfused in toto (PBS; 10mL) and fixed (2% formaldehyde EMS #15710, 2.5% glutaraldehyde EMS #16220 in 0.1M Phosphate Buffer). Kidneys were harvested and fixed in the same solution ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with CyFACS and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS (Rockland) ...
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bioRxiv - Biophysics 2021Quote: ... and permeabilized for 2 min with 0.3% glutaraldehyde (GA) + 0.25% Triton X-100 (EMS, 16220 and Thermo Fisher, 28314) in pre-warmed (37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and rinsed in distilled water prior to en bloc staining with 2% uranyl acetate (22400, Electron Microscopy Sciences, USA). After dehydration through a graded ethanol series ...
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bioRxiv - Neuroscience 2021Quote: One 5 mm sample from each naïve nerve was fixed for 48 hours at 4° C in a solution of 2% glutaraldehyde (16216, EMS), 3% paraformalydehyde (15754-S ...
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bioRxiv - Biochemistry 2021Quote: ... Ycf1 sample was applied to a CFlat-1.2/1.3-4C-T (WT Ycf1) or QF-1.2/1.3-4Au grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10°C and 80% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... Brain slices were washed extensively in cacodylate buffer at room temperature and stained sequentially with 2% osmium tetroxide (EMS) in cacodylate buffer ...
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bioRxiv - Microbiology 2021Quote: ... the grids were washed with PBS three times before being fixed with 2% EM grade paraformaldehyde (EMS, EM Grade) for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... infected and uninfected cells were fixed by rapidly transferring Aclar slabs into Karnovsky’s fixative (2% Glutaraldehyde (EMS cat.#16000) and 4% PFA (EMS cat.#15700 ...
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bioRxiv - Molecular Biology 2021Quote: Eyes were fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer (all from Electron Microscopy Sciences) overnight with gentle rocking at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed and then fixed in FACS buffer supplemented with 2% PFA (15710-S, Electron Microscopy Sciences) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Etched coverslips were cleaned of oil and fixed in a 2% solution of glutaraldehyde in PBS (Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2023Quote: ... the cells were washed with cold flow cytometry buffer and fixed with 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA). The samples were stored at 4°C wrapped in foil up to 1 week prior to flow cytometric analysis ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were then stained with a 2% (w/v) solution of uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). High-resolution TEM images were captured using a JEOL JEM-2010 TEM and JEOL JEM-2100 Field Emission Gun TEM ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 3X with CyFACS buffer and fixed overnight at 4°C with 2% PFA (Electron Microscopy Sciences) in metal contaminant-free PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was then stained by adding 10 µL of 2% filtered uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Grids were then blotted dry with filter paper and stored at RT and visualized by using a bottom-mounted Eagle 4k x 4k camera on a Tecnai F20 TEM (FEI Company ...
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bioRxiv - Cell Biology 2022Quote: ... placed on 200 mesh copper TEM grids and were stained with 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA) and Reynold’s lead citrate (Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and then stained in 1.0% osmium tetroxide (cat. no. 19100 Electron Microscopy Sciences) for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... followed by embedding in 2% agarose and post-fixed in 0.1 M osmium tetroxide (SKU 19152, Electron Microscopy Science). Dehydration was achieved through a series of acetone solutions with increasing concentrations ...
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bioRxiv - Cell Biology 2022Quote: ... before being fixed by perfusion using 0.15M cacodylate buffer (pH 7.4) containing 2% formaldehyde and 2.5% glutaraldehyde (Electron Microscopy Sciences) as well as 2mM CaCl2 at 35°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... four to five serial sections were picked up per 2 × 1 mm formvar and carbon coated slot grid (EMS). Because the Aclar coverslip ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... cells were rinsed with PBS containing calcium and magnesium and fixed using 2% paraformaldehyde (PFA) (Electron Microscopy Sciences # 15710) in PBS for 20 min ...