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Citations for Electron Microscopy Sciences :
601 - 650 of 1075 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: 3 μg of purified NHE9*CC sample was applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences) and blotted for 3.0s with a 20s waiting time prior ...
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bioRxiv - Immunology 2024Quote: ... At -60 °C samples were infiltrated with increasing concentrations (30, 60, and 100% for 1 h each) of Lowicryl (3:1 K11M/HM20 mixture; Electron Microscopy Sciences). After 5 h of 100% Lowicryl infiltration ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of this sample at 2 µg Chl mL−1 was applied to a glow discharged (60 s, 25 mA) 400 mesh Cu grid with 5-6 nm formvar with 3-4 nm carbon (Electron Microscopy Sciences). The sample was negatively stained with 2% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... amylovora strain Ea110 were collected at 3 dpi and fixed in paraformaldehyde/ glutaraldehyde (2.5% of each compound in 0.1 M sodium cacodylate buffer) (Electron Microscopy Sciences, Hatfield, PA) at 25 C overnight ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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bioRxiv - Cancer Biology 2021Quote: ... and on a 30 μL droplet of 2% uranyl acetate solution (Electron Microscopy Sciences, Hatfield, PA) for 10 additional minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... The coverslips were then moved immediately into unroofing buffer containing 2% glutaraldehyde (Electron Microscopy Sciences, 16019). With syringe ...
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bioRxiv - Biochemistry 2020Quote: ... cells at room temperature were fixed for 10 minutes with 2% formaldehyde (Electron Microscopy Sciences 15710), and permeabilized for one hour with 0.1% Triton X-100 (Biorad 1610407 ...
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bioRxiv - Neuroscience 2021Quote: ... sapphire disks were placed into 12-well plate containing 1 mL of 2% glutaraldehyde (EMS #16530) and 1 mM CaCl2 (Sigma-Aldrich # 63535 ...
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bioRxiv - Physiology 2020Quote: ... and stained with freshly prepared 2% aqueous solution of Osmium Tetroxide (Electron Microscopy Sciences, Cat#19150) in 0.1 M sodium cacodylate buffer (pH 7.4) ...
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bioRxiv - Cell Biology 2020Quote: ... and manually unroofed with 19-gauge needle and syringe using 2% paraformaldehyde (Electron Microscopy Sciences 15710) in intracellular buffer ...
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bioRxiv - Neuroscience 2022Quote: ... mice were sacrificed by transcardial perfusion with Karnovsky’s fixative: 2% glutaraldehyde (Electron Microscopy Sciences (EMS 16000) and 4% paraformaldehyde (EMS 15700 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were sacrificed by transcardial perfusion with Karnovsky’s fixative: 2% glutaraldehyde (Electron Microscopy Sciences (EMS 16000) and 4% paraformaldehyde (EMS 15700 ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed with 1XPBS and fixed for 15 min with 2% paraformaldehyde (Electron Microscopy Sciences) in 1XPBS ...
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bioRxiv - Cell Biology 2022Quote: ... The sample was subsequently imaged by FLIM or stored in 2% glutaraldehyde (Electron Microscopy Sciences, 16019) at 4°C until EM sample preparation.
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bioRxiv - Cell Biology 2022Quote: ... and manually unroofed with 19-gauge needle and syringe using 2% paraformaldehyde (Electron Microscopy Sciences, 15710) in the intracellular buffer ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed using 300-400 µl of 2% paraformaldehyde (Electron Microscopy Sciences, catalog 15712-S), then CD107a surface expression was measured using a modified LSR Fortessa (BD Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples from fish 60dpf and older were treated with 2% osmium tetroxide (19130, Electron Microscopy Sciences), dehydrated in increasing ethanol series and by critical point drying ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixated in a 96U plate in 250 µl of 2% paraformaldehyde (PFA, EMS #15710) in PBS at room temperature for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... and post-fixed for one hour with 2% osmium tetroxide (Electron Microscopy Science, USA, cat. 19190) in 0.15 M of phosphate buffer ...
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bioRxiv - Genetics 2020Quote: ... cells were relaxed using 50mM KCl and then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS, #16000) and 4% paraformaldehyde (EMS ...
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bioRxiv - Genetics 2020Quote: ... sections were mounted with Fluoromount-G containing 4′,6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences). Staining was visualized and imaged using a Nikon 80i microscope with a Nikon DS-Fi1 camera or a Nikon Eclipse TiE attached to an A1R-SHR confocal ...
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bioRxiv - Cell Biology 2021Quote: ... post-fixed in 2 mL 1% osmium tetroxide (OsO4) (Electron Microscopy Sciences, Hatfield, PA, Catalog #19150) in 0.15 M sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... post fixed them for 2 hours in 1% osmium tetroxide (Electron Microscopy Sciences, cat n. 19150) 0.2 cacodylate sucrose buffer and washed them 1 time with buffer ...
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bioRxiv - Genomics 2020Quote: ... and the cells were stained with reduced osmium (2% osmium tetroxide and 1.5% potassium ferrocyanide, EMS) for 30 minutes on ice to further enhance contrast ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed in 4% paraformaldehyde and 2% sucrose in PBS for 10 mins (EMS Diasum). Fixed cells were permeabilized/blocked in 0.2% (v/v ...
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bioRxiv - Bioengineering 2022Quote: ... the coverslips with cells were incubated in 2% osmium tetroxide (Electron Microscopy Sciences, Hatfield PA, US) for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... After washing in buffer and two hours in post-fixating 2% osmium tetroxide (Electron Microscopy sciences) in 0.1M sodium cacodylate (Electron Microscopy sciences ...
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bioRxiv - Microbiology 2022Quote: ... Electron Microscopy Sciences) and 2% lead citrate (in distilled water; Electron Microscopy Sciences, Hatfield, PA, USA) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin-impregnated precipitates were placed in gelatin capsules (size 2, Electron Microscopy Sciences, United States), filled with fresh resin ...
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bioRxiv - Neuroscience 2024Quote: ... the brain underwent 2 washes with 0.2 M sodium cacodylate buffer (pH 7.2, Electron Microscopy Science), each lasting 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... washed again with PBS + 2% FBS and fixated in PBS + PFA (4:100, Electron Microscopy Sciences).
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bioRxiv - Genetics 2023Quote: ... the pellet was submitted to post fixation using 1% Osmium tetroxide 2% (OsO4, Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer for 30 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The ultrathin sections were then contrasted with aqueous 2% uranyl-acetate (EMS, Science Services, Munich, Germany) and 2% lead-citrate solution (Leica Ultrostain II ...
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bioRxiv - Neuroscience 2023Quote: Cells were grown on aclar and then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% para-formaldehyde (EMS Cat# 15700 ...
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bioRxiv - Neuroscience 2023Quote: ... fixed with 4% PFA for 1 min and stained with 2% uranyl acetate (Electron Microscopy Sciences) for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... sponges were fixed for 2 hours at 4 °C with 2.5% glutaraldehyde (Electron Microscopy Science, 16020), post-fixed for 1 hour at room temperature with 1% OsO4 (Electron Microscopy Science ...
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bioRxiv - Cell Biology 2024Quote: ... For TEM cells were fixed (in plates) in 2% paraformaldehyde and 1% glutaraldehyde (Electron Microscopy Sciences) in 0.1 M sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... or 2% paraformaldehyde/2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences, 15960-01) with 1% tannic acid (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: Organ of Corti explants were fixed in 2% paraformaldehyde/ 2.5% glutaraldehyde (Electron Microscopy Sciences, 15960-01) supplemented in 1% tannic acid (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Slides were fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium ...
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bioRxiv - Biochemistry 2024Quote: Medial gastrocnemius (MG) muscle was dissected and fixed in freshly prepared 2% paraformaldehyde (Electron Microscopy Sciences) in phosphate-buffered saline (PBS ...
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Immune organization in sentinel lymph nodes of melanoma patients is prognostic of distant metastasesbioRxiv - Cancer Biology 2024Quote: ... Slides were fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium ...
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bioRxiv - Bioengineering 2020Quote: ... the samples were washed for 3 times with ice-cold H2O and then incubated overnight in the dark in 1% uranyl acetate (Electron Microscopy Sciences, 22400) at 4°C.
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
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bioRxiv - Neuroscience 2022Quote: Brains were dissected from female flies 1-3 days post-eclosion in Drosophila external saline and fixed in 4% paraformaldehyde (Electron Microscopy Sciences, #15714) in phosphate-buffered saline (PBS ...