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Citations for Akoya Biosciences :
751 - 800 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and TSA Plus Cy5 System (Akoya Biosciences, NEL745001KT). Coverslips were mounted with ProLong Diamond Antifade (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... Dyes used for detection were TSA Plus Cy3 System (Akoya Biosciences, NEL744001KT) and TSA Plus Cy5 System (Akoya Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... the development reaction was carried out using the TSA Plus Cyanine 3 Kit (Akoya Biosciences, US) (FISH ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-slide scans were performed at 20x magnification utilizing the PhenoImager HT automated multispectral imaging system (Akoya Bioscience, Marlborough, MA). BF Npas1+ efferent regions (AAV-EYFP anterograde projections ...
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bioRxiv - Neuroscience 2023Quote: ... 620 (Akoya Biosciences FP1495001KT) and 690 (Akoya Biosciences FP1497001KT ...
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bioRxiv - Neuroscience 2023Quote: ... Signals were amplified by TSA-plus Biotin (AKOYA Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies (described above for western blotting ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and spectral DAPI (Akoya Biosciences).
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bioRxiv - Bioengineering 2023Quote: The antibody conjugation and the staining were done according to the manufacturer’s protocol (Akoya Biosciences). Details regarding primary antibody ...
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bioRxiv - Bioengineering 2023Quote: ... and Opal 650 and TSA-biotin (TSA Plus Biotin Kit) (Akoya Biosciences) and streptavidin-conjugated Atto 425 (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Images were taken using the Vectra® Polaris™ work station (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... Opal Anti-Ms + Rb HRP (Akoya Biosciences, Marlborough, MA ARH1001EA), before conjugating with Opal fluorophores Following incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were scanned and quality of staining assessed after multiplex IHC staining using the Phenochart software (v1.1.0, Akoya Biosciences). The images were then exported for further analysis into QuPath (v0.4.3) ...
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bioRxiv - Cancer Biology 2023Quote: Multiplex immunofluorescence images were acquired using the Mantra™ Quantitative Pathology work station (Akoya Biosciences). The resulting multispectral slide scan bands (MOTiF ...
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bioRxiv - Cancer Biology 2023Quote: ... antibodies were conjugated using Akoya Antibody Conjugation Kit (Akoya Biosciences, SKU 7000009) with a barcode (Akoya Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... the region and the z-stack were configurated on the Keyence software and the number of cycles with targets were designated in the Akoya’s PhenoCycler® Instrument Manager (version 1.29.3.6) as described in the CODEX user manual (Akoya Biosciences, 2021a). Acquired images were processed ...
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bioRxiv - Cancer Biology 2023Quote: ... and imaged with Vectra Polaris/PhenoImager HT (Akoya).
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bioRxiv - Bioengineering 2023Quote: ... and segmented using the CODEX Processor set at default manufacturer values with a radius of 10 for segmentation (Akoya Biosciences, 2021b). Final data were then viewed using the CODEX Multiplex Analysis Viewer (MAV ...
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bioRxiv - Bioengineering 2023Quote: ... Final data were then viewed using the CODEX Multiplex Analysis Viewer (MAV) plugin (Akoya Biosciences, 2021c) for FIJI (ImageJ)31 and the EnableMedicine Visualizer tool (beta version).
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bioRxiv - Cancer Biology 2023Quote: ... Then a staining dye was added after secondary antibody washes: α-SMA – Opal-dye 520 (Akoya Biosciences OP-001001) at 1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... spectral unmixing and background subtraction were performed by creating an algorithm for this specific staining and tissue (tumor, breast) using inForm (version 2.8; Akoya Bioscience). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... A spectral library was created on InForm Analysis Software (Akoya Biosciences) for the quantitative analysis of cell populations ...
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bioRxiv - Cancer Biology 2023Quote: ... *20/0.8 objective (3DHISTECH) or with a Phenocycler scanner (Akoya Biosciences). Images were analyzed using QuPath software (Bankhead et al ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Opal Multiplex IF Kit (Akoya) was used to perform multiplex immunofluorescence according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Imaging was performed at 20× using a Vectra multispectral imaging system (Akoya Biosciences) and a spectral library was generated to separate spectral curves for each fluorophore ...
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bioRxiv - Cancer Biology 2023Quote: ... Images were taken with a Phenocycler scanner (Akoya®) and analyzed using QuPath software ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Developmental Biology 2023Quote: ... Opal dyes (Akoya Biosciences ...
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bioRxiv - Immunology 2023Quote: ... were generated for affiliated brightfield and multiplex fluorescent immunohistochemistry (mfIHC) panels were generated using a multispectral Vectra Polaris Automated Quantitative Pathology Image System (Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... and TSA TMR Reagent (cat. # SAT702001, Akoya Biosciences). The following antibodies were used ...
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bioRxiv - Cancer Biology 2023Quote: ... the CODEX instrument (Akoya Biosciences, USA), and CODEX Instrument Manager (CIM ...
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bioRxiv - Cancer Biology 2023Quote: ... and CODEX Instrument Manager (CIM) (Akoya Biosciences, USA). Exposure times ...
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bioRxiv - Cancer Biology 2023Quote: ... The raw images were then stitched and processed using the CODEX processor (Akoya Biosciences, USA). After multiplex imaging was completed ...
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bioRxiv - Pathology 2023Quote: ... and Opal 650 dyes (Akoya Biosciences). No nuclear stain was applied at this stage ...
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bioRxiv - Immunology 2023Quote: mIF stainings using the Opal dyes from Akoya were digitized with multispectral imaging by the Vectra® Polaris™ (PerkinElmer ...
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bioRxiv - Cancer Biology 2023Quote: Reagents which are purchased from Akoya and used “as-is” will be annotated as (A) ...
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bioRxiv - Cancer Biology 2023Quote: Multispectral imaging was performed using PhenoImager at 20× magnification (Akoya Biosciences, Marlborough, MA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the multispectral acquired images were loaded into InForm software for unmixing and background subtraction (inForm v.3.0; Akoya Biosciences). In the inForm software ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated with TSA Opal650 (Akoya Biosciences) diluted 1/1500 ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were washed and anti-mouse/anti-rabbit HRP polymer (Akoya Biosciences) was added for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The vGLUT2 immunoreaction was enhanced with tyramide signal amplification (1:50; Akoya Biosciences). Next ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 570 (1:1500, Akoya FP1488001KT), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Reagents that are purchased from Akoya but need preparation prior to use will be underlined and annotated as (A).
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bioRxiv - Genomics 2023Quote: ... and Opal 650 (Akoya Biosciences) and TSA-biotin (TSA Plus Biotin Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent oligonucleotides (Akoya Biosciences) were added to this reporter solution at a final concentration of 1:50 in a total of 250 μl per well ...
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bioRxiv - Developmental Biology 2023Quote: Raw tiff files were processed using the CODEX Processor version 1.7.0.6 by Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... The screening buffer was prepared according to the CODEX manual provided by Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...