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Citations for Akoya Biosciences :
951 - 1000 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Reporter stock solution was prepared containing 1:300 Hoechst stain and 1:12 dilution of assay reagent in 1x CODEX buffer (Akoya Bioscience). Fluorescent oligonucleotides (Akoya Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were washed twice in hydration buffer for a total of 4 minutes followed by fixation in storage buffer (Akoya Bioscience) with 1.6% paraformaldehyde for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... All images were processed using the CODEX processor (Akoya Bioscience). Numbers of Ki67 ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was then allowed to dry for 2 minutes and hydrated in hydration buffer (Akoya Biosciences) twice for 2 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Automated image acquisition and fluidics was performed using Akoya’s software driver CODEX Instrument Manager (CIM) and the CODEX platform (Akoya Bioscience). Imaging was performed using a Keyence BZ-X800 microscope ...
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bioRxiv - Cancer Biology 2022Quote: ... CODEX fixative solution (Akoya Bioscience) was prepared right before the final fixation step ...
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bioRxiv - Cancer Biology 2022Quote: ... Fluorescent oligonucleotides (Akoya Bioscience) were added to this reporter solution at a final concentration of 1:50 in a total of 250 µl per well ...
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bioRxiv - Cancer Biology 2022Quote: ... J and G blocking solutions to CODEX staining buffer (Akoya Bioscience). Antibodies were then added to this blocking buffer in a 1:50 titration to make a total volume of 100 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... This was followed by two washes in hydration buffer and equilibration in staining buffer (Akoya Biosciences) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Immunology 2022Quote: ... and Ki67 (Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent ...
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bioRxiv - Neuroscience 2022Quote: ... amplification was achieved using TSA Biotin (Akoya Bioscience). For fluorescence double in situ hybridization ...
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bioRxiv - Neuroscience 2022Quote: ... Akoya Bioscience) followed by TSA-plus cyanine 3 or TSA-plus fluorescein (Akoya Bioscience). When required ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
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bioRxiv - Pathology 2022Quote: ... were used as a second step for 45 minutes at room temperature Staining for α-SMA was then developed by Opal650 tyramide (Akoya Biosciences, Marlborough MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... using CODEX conjugation kit (Cat#: 7000009, Akoya) following CODEX conjugation manual.
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bioRxiv - Pathology 2022Quote: ... was conjugated with a barcode-oligonucleotide (Cat#: 5350002, Akoya) using CODEX conjugation kit (Cat# ...
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bioRxiv - Microbiology 2022Quote: ... Opal dyes (Akoya Biosciences, MA) were used for secondary staining as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... The hybridization detection was carried out using Opal Fluorophore Reagent Packs Opal-520 (channel (C) 1)/570 (C2)/690 (C3) (1:1500) (Akoya Biosciences). Probes (Advanced Cell Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... Entire sections were scanned with 40x magnification using a Vectra Polaris quantitative slide scanner (Akoya Biosciences, MA, US) for subsequent bacterial co-localisation and cell number analysis ...
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bioRxiv - Microbiology 2022Quote: ... Scanned section image analysis was performed using Fiji (v1.53)[21] and inForm image analysis software (v2.5.1; Akoya Biosciences). For determination of the area of pneumococci associated to each macrophage subtype ...
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bioRxiv - Genetics 2022Quote: ... samples were incubated for 30 min at 40°C with Opal 620 (AKOYA Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... and segmented using the CODEX Processor set at default manufacturer values (Akoya Biosciences, 2021b). Final data were then viewed using the CODEX Multiplex Analysis Viewer (MAV ...
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bioRxiv - Neuroscience 2022Quote: ... Final data were then viewed using the CODEX Multiplex Analysis Viewer (MAV) plugin (Akoya Biosciences, 2021c) for FIJI (ImageJ ...
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bioRxiv - Neuroscience 2022Quote: ... CODEX reporter 96-well plates were prepared using the cycle reporter configuration as outlined in the CODEX user manual (Akoya Biosciences, 2021a). Imaging was performed using a Keyence BZ-X800 epifluorescence microscope equipped with either a 20x objective (Nikon CFI Plan Apo 20x/0.75 ...
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bioRxiv - Cancer Biology 2022Quote: ... TSA reagents were diluted with 1×□Plus Amplification Diluent (PerkinElmer/Akoya Biosciences, US).
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bioRxiv - Cell Biology 2022Quote: ... (Akoya Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal 570 (Akoya FP1488001KT), and Opal 650 (Akoya FP1496001KT ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were imaged at both 10x and 20x using the Vectra 3 Polaris and Vectra imaging software (Akoya Biosciences). Acquired qpTIFF images from the Vectra Polaris system were imported into QuPath analysis software(84 ...
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bioRxiv - Neuroscience 2022Quote: ... then 150 µL of Opal 570 (Akoya, FP1488001KT) dye diluted in RNAscope TSA diluent (ACD ...
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bioRxiv - Bioengineering 2022Quote: ... The fluorescent Opal 570 dye was added to channel 2 containing SLC17A7-C2 (excitation: 550 nm, emission: 570 nm; Akoya Biosciences). DAPI was added in the final step to spheroids for 30-sec ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Bioengineering 2022Quote: ... Opal 650 (Akoya, FP1496001KT) for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... Opal 570 (Akoya, FP1488001KT), Opal 650 (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: A custom antibody panel was developed and validated (Enable Medicine, Menlo Park, CA, USA) for multiplexed imaging with co-detection by indexing (CODEX) (Akoya Biosciences, Menlo Park, CA, USA). This imaging technique utilizes antibodies conjugated to unique DNA oligonucleotide barcodes ...
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bioRxiv - Cancer Biology 2022Quote: CODEX imaging was done as per the manufacturer’s protocol (Akoya Biosciences). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... The final tissue fixation was performed with the Fixative reagent (Akoya Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... and fluidics exchange managed via the CODEX instrument and CODEX Instrument Manager software (CIM version 1.29.3.6, Akoya Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... deconvolved and background subtracted utilizing the CODEX Processor Software (Akoya Biosciences), and antibody staining was visually assessed for each biomarker and tissue region using the ImageJ software (Fiji ...
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bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Neuroscience 2022Quote: ... Each probe was then developed by sequential application of the appropriate HRP reagent (HRP-C1, HRP-C2) for 15 minutes each at 40°C and fluorophore (Akoya Biosciences Opal 690 and Opal 520) for 30 minutes each at 40°C ...
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bioRxiv - Genomics 2022Quote: A total of 32 antibodies were used for CODEX and 19 of them were conjugated in-house using a protocol outlined by Akoya Biosciences (Akoya Biosciences ...
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bioRxiv - Genomics 2022Quote: ... The resulting images were processed using the CODEX processing software (Akoya Biosciences, Menlo Park, CA) and visualized using FIJI/ImageJ.
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bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Multispectral images were analyzed using the inForm software version 2.4 (Akoya Biosciences).
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 690 fluorophore (Akoya Biosciences, 1:50). Finally ...
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bioRxiv - Immunology 2022Quote: ... regions of interest defined using Phenochart software (Akoya Biosciences), and multispectral images acquired at x200 magnification using the Ventra 3.0 Automated Imaging System (Akoya Biosciences) ...