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Citations for Eppendorf :
801 - 850 of 911 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were dissolved in 0.1% RapiGest and 500 mmol/L TEAB and then incubated at 850 rpm for 45 min at 37 °C (Eppendorf Comfort Thermomixer, Eppendorf, USA). Proteins were digested by trypsin (Trypsin Gold ...
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bioRxiv - Biochemistry 2022Quote: ... and incubated with Methoxamine hydrochloride (MeOX-HCl, 2%, 40 μl) at 60 °C for 2 hours at 400 rpm in a thermomixer (Eppendorf, USA). After adding N-methyl-N-(trimethylsilyl ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Cell Biology 2023Quote: ... The sample was then concentrated on a 1.2 µm size meshed mixed cellulose ester membrane (Merk) and pelleted using centrifugation for 5 minutes at 1000G and 20°C with a swinging bucket centrifuge (Eppendorf 5427R).
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Cell Biology 2023Quote: ... or at 37°C/5% CO2/1% O2 in a nitrogen-controlled hypoxic incubator (New Brunswick Galaxy 170R, Eppendorf, Hamburg, Germany). Prior to media changes ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted samples were aliquoted in 2 mL tubes and vaporized under vacuum at 40°C using SpeedVac (Eppendorf, Hamburg, Germany). The samples were resuspended and further diluted in sterile water before applying to MBA ...
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bioRxiv - Genetics 2023Quote: ... Plates were sealed with Parafilm and incubated for 6 days at 20°C while shaking at 200 rpm (New Brunswick I26/I26R shaker, Eppendorf). On day six the plates were observed under a dissection microscope and the survival and reproduction phenotypes were assessed in each condition ...
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bioRxiv - Plant Biology 2023Quote: ... was added to frozen cell pellets and incubated for 10 min at 65°C and 1000 rpm in a Thermomixer (Eppendorf, Germany). The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C ...
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bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were kept at 37 °C and 5% CO2 in a humidified incubator (Galaxy 170 S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80% ...
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bioRxiv - Cell Biology 2023Quote: ... the resuspended cilia were centrifuged at 16000 × g for 10 minutes at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D), and the supernatant was removed ...
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bioRxiv - Microbiology 2024Quote: ... Cells grown under photoautotrophic and dark heterotrophic conditions were collected using centrifugation (6,000 rpm, 10 min, 4°C; R10A2 rotor, Himac CR21F, Eppendorf Himac Technologies) and washed with a small amount of PBS ...
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bioRxiv - Microbiology 2024Quote: ... 50 mL culture was centrifuged for 5 min at 18 °C and 3,220 g (5810 R swing-out centrifuge; Eppendorf, Hamburg, Germany) and the supernatant was removed by vacuum suction ...
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bioRxiv - Neuroscience 2024Quote: ... The forebrain was dissected and weighed before lyophilization at a shelf temperature of –56 °C for 24h under vacuum of 0.2 mBar (Christ LMC-1 BETA 1-16) and extraction with formamide at 57°C for 24h on a shaker at 300 rpm (Eppendorf Thermomixer). Integrated density of tracer fluorescence was determined in triplicates after 1:3 ethanol dilutions ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were washed with cold 70% ethanol and centrifuged at 19,000 × g for 5 min at 4°C and air dried for 15 min in the Centrifugal Vacuum Concentrator 5301 (Eppendorf, USA). The quality of RNA was assessed using a NanoDrop (ND-1000 ...
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bioRxiv - Biochemistry 2024Quote: ... and the sample was incubated at 60 °C and 1,000 rpm for 30 minutes on an Eppendorf ThermoMixer Temperature Control Device (Eppendorf, Hamburg, Germany). Iodoacetamide (IAA ...
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bioRxiv - Microbiology 2024Quote: ... The stationary pre-cultures were then pooled and washed by centrifugation (5 min, 3,220 g and 4 °C) using a 5810 R swing-out centrifuge (Eppendorf, Hamburg, Germany). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... The crude EV fractions were prepared by ultracentrifugation (45,000 rpm, 1 h, 4°C; S50A rotor, Himac Ultracentrifuge CS100GXII; Eppendorf Himac Technologies) of the thus prepared supernatants.
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bioRxiv - Cell Biology 2020Quote: ... The samples were kept soaked at 37 °C for up to 7 days on an orbital shaker (Excella E24, Eppendorf, Hamburg, Germany) with an agitation rate of 150 rpm ...
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bioRxiv - Evolutionary Biology 2019Quote: ... pre-loaded with 70µl/well of bacterized CM and incubated at 43°C for 1.5 min in a thermocycler (Eppendorf Mastercycler® pro S). Cells were randomly assigned to plate wells for both control (no heat shock ...
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bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting precipitates were centrifuged for 15 minutes at 25000 relative centrifugal force (rcf) in a 5417R Eppendorf centrifuge at 4°C (Eppendorf, Hamburg, Germany). The resultant supernatants were equilibrated to room temperature and then carefully drawn and added into fresh 1.5ml tubes containing 400ml of isopropanol ...
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bioRxiv - Genomics 2020Quote: ... We included an overnight digestion step in which tissues were lysed in a 96-well PCR plate at a constant temperature of 55° C on an Eppendorf Mastercycler thermal cycler (Eppendorf, Hamburg, Germany), and we included 10 uL Proteinase K solution ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf; EP0030700112-300EA) upon reaching high density ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were eluted with 60 μl of 80% acetonitrile/1% ammonia and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were dissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2020Quote: ... The cell suspension was subsequently centrifuged for 30 min at 4°C and 3250 × g (Eppendorf Swing-bucket rotor A-4-62). The supernatant was discarded ...
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bioRxiv - Systems Biology 2021Quote: ... Total volume of 20 mL from each culture was transferred to a 50 mL Falcon® filled with ice and was immediately centrifuged at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). Next the supernatant was discarded and the cell pellet was snap frozen into liquid nitrogen and stored at -80 °C ...
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bioRxiv - Systems Biology 2021Quote: For the extraction of total proteome 10 mL of each culture were transferred into ice-cold 15 mL Falcon® tubes which were centrifuged immediately at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). The supernatant from the centrifugation was discarded and the cell pellets were washed once with 1 mL of cold PBS buffer ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... Approximately 22.5×107 suspension-adapted Expi293F cells were diluted in Expi293 Expression Medium to the final volume of 76.5 ml and cultured in a 37°C shaker (Eppendorf New Brunswick S41i). 90µg of plasmid DNA was mixed with 240µl Expifectamine reagent into a total final volume of 9ml OPTI-MEM medium and was incubated for 25 min ...
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bioRxiv - Genetics 2022Quote: ... we inoculated yeast strains into 400 µL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30 °C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day ...
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bioRxiv - Synthetic Biology 2022Quote: ... The fluorocarbon/ethanol mix was shaken at room temperature for 16 h with 1000 rpm using an Eppendorf ThermoMixer C (Eppendorf AG, Germany) to perform the liquid-liquid extraction ...
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bioRxiv - Systems Biology 2022Quote: ... Peptides were eluted with 60 μl of elution buffer (80% acetonitrile/1% ammonia) and dried at 60°C using a SpeedVac centrifuge (Eppendorf, Concentrator plus). Dried peptides were redissolved and sonicated in 5% acetonitrile/0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.4) with and without 1,4 dithiothreitol (DTT) (10 mM) and agitated at 400 oscillations per minute (Eppendorf Thermomixer C, Eppendorf, Germany) for 48 h.
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bioRxiv - Plant Biology 2021Quote: ... 0.1 M NaHCO3) by incubating at 65°C for 15 min at 1200 rpm in a ThermoMixer Comfort (Eppendorf AG, Hamburg, Germany). NaCl was added to the eluates and the input DNA aliquots to a final concentration of 0.2 M ...
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bioRxiv - Cancer Biology 2020Quote: ... sonicated for 15 min and vigorously mixed at 5°C for 30 min with 2000 rpm on a ThermoMixer (Eppendorf, Hamburg, Germany). Samples were centrifuged for 5 min at 18000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... 4.6×106 LMBs or NMBs were added and samples incubated at 37 °C for 1 hour on a thermomixer (60 rpm; Eppendorf, Hamburg, Germany). After this step ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 50 pmols of each primer set (up to two channels per experiment) and 10% formamide] 16-48 hrs at 37°C mixing at 400 rpm in a thermomixer (Eppendorf ThermoMixer F1.5). Embryos were washed for 30 min in Stellaris Wash Buffer A ...
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bioRxiv - Genomics 2022Quote: ... The remaining PIP emulsion was subjected to enzymatic lysis at 65°C for 35 min on a PCR thermocycler (Eppendorf Mastercycler Pro) with lid temperature set to 105°C ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were pelleted by centrifugation at approximately 250 × g for 5 min at 4 °C (Eppendorf 5804 R, Hamburg, Germany). After resuspension ...
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bioRxiv - Synthetic Biology 2019Quote: ... was added to each library and incubated for two hours at room temperature while shaking using a Thermomixer C (Eppendorf, Hamburg, Germany). M2B2 beads (US Biological ...
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bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
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bioRxiv - Microbiology 2020Quote: ... Liquid cultures were grown at 29°C and shaken at 200 and 180 rpm in New Brunswick Innova 2300 and Excella E25 shaking incubators (Eppendorf, Hauppauge, NY), respectively ...
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bioRxiv - Bioengineering 2021Quote: ... of stock cells were grown overnight in 5 mL of LB at 37°C and 200 rpm on a 75° angled platform in a New Brunswick Excella E25 (Eppendorf, CT, USA). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... Lysates (5 μg) were incubated with diverse ZnCl2 or PP2A inhibitors for 30 min at 30 °C using a Thermo-mixer (Eppendorf, Hamburg, Germany). The protein samples were incubated for 30 min at 33 °C with a chemically synthesized phosphopeptide [RRA(pT)VA] ...
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bioRxiv - Microbiology 2022Quote: ... 20 µl supernatant was mixed with 180 µl substrate solution and incubated for 10 min at 37°C and 1000 rpm in a thermomixer (Eppendorf Thermomixer comfort). The reaction was stopped by addition of 60 µl 1 M sodium hydroxide ...
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bioRxiv - Immunology 2022Quote: ... Purified IgG was digested into F(ab’)2 with 200 μg of IdeS per 10 mg of IgG for 6 hr on a thermal mixer (Eppendorf ThermoMixer C) at 37°C ...