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Citations for Eppendorf :
701 - 750 of 911 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Excised gel slices were incubated with 50% acetonitrile/50mM ammonium bicarbonate for 2 h at room temperature or 4°C overnight with gentle shaking at 600 rpm in a Thermomixer (Eppendorf), before being incubated in 100% acetonitrile for 15 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Bioengineering 2019Quote: For extract preparation each 4 mL of cell cultures from tp 48h of HDC run 1 were spun down for 10 min at 4000 g and 4 °C (Centrifuge 5804R, Rotor A-4-44, Eppendorf). Pellets were resuspended in fresh 1 mL ‘thylakoid buffer’ (50 mM HEPES-NaOH ...
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bioRxiv - Bioengineering 2019Quote: ... the crude extracts were transferred into a fresh tube and centrifuged for 30 min at 15000g and 4 °C (Centrifuge 5427R, Eppendorf). The soluble supernatant (blue color ...
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bioRxiv - Genomics 2019Quote: ... The samples were then incubated for 10 minutes at 65°C and 1000 rpm in a Thermomixer (Eppendorf, Hamburg, Germany) before cooling on ice for 1 minute ...
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bioRxiv - Biophysics 2019Quote: ... Samples were then incubated for 30 min at 45°C and 500 rpm using ThermoMixer® (Eppendorf, Wesseling/Berzdorf, Germany). After incubation ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation reactions were carried out for the indicated proteins using rabbit reticulocyte lysate and SP cells at a final concentration of 3×106 cells/ml at 30°C for 40 min in a thermomixer (Eppendorf) set to 900 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with 400 μL ChIP elution buffer (containing 50 μg RNase A) and incubated at 37 °C at 1,200 rpm on a Thermomixer (Eppendorf, Germany) for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: All cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). They have also been regularly tested for mycoplasma contamination by examining the samples for extracellular DNA staining with SiR-DNA (100 nM ...
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bioRxiv - Biochemistry 2022Quote: ... These samples were reduced by addition of dithiothreitol (DTT) to 10 mM final concentration and incubation at 37 °C with agitation (700 rpm) in a benchtop ThermoMixer (Eppendorf) for 45 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new tube and solvents were removed by evaporation at 30°C for 90 min at reduced pressure (Concentrator Plus, Eppendorf). The samples were then fully dried in a freeze dryer and stored at −80°C ...
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bioRxiv - Cell Biology 2022Quote: ... Sorted nuclei were then centrifuged at 1000 g for 15 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3), and supernatant was removed ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were then co-incubated with bacterial strains at an MOI of 1:1 ratio in RPMI 1640 cell culture medium (HIMEDIA) and incubated at 37⁰C in an animal tissue culture incubator (Eppendorf). The same method was performed for macrophage RAW 264.7 cell line in the DMEM medium (HIMEDIA) ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... and Sample Reducing Agent (10x) and incubated for 5 min at 70°C and 1100 rpm using a ThermoMixer comfort (Eppendorf).
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged at 16,100 g for 20 min at 4 °C with an Eppendorf 5415R centrifuge (Eppendorf, Hamburg, Germany), sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... and streptomycin (10 µg ml−1) at 37° C for 40 min with shaking at 800 rpm in a thermomixer (Eppendorf). Digested medullae were resuspended and the enzymatic reaction quenched by addition of 10 volumes cold CMF-HBSS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... in 200 µl volumes grown at 37 °C with 200 rpm shaking in an Innova 42R Incubator (Eppendorf, United Kingdom) for 22 h growth periods (‘days’) ...
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bioRxiv - Systems Biology 2023Quote: ... Saponified non-polar metabolites were obtained by modifying the protocol with the addition of 60% KOH (10X) in 50% methanol followed by an incubation at 42°C for 1 h in a thermomixer (300 rpm Eppendorf), instead of the 1 h rotation at RT ...
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bioRxiv - Systems Biology 2023Quote: ... Homogenized islets were filtered with 30 µm filter (CellTrics, Sysmex) and pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei were resuspended in 400 µL of sort buffer (1% fatty acid-free BSA ...
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bioRxiv - Systems Biology 2023Quote: ... were added without disturbing the pellet and nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed without disturbing the pellet and leaving ∼2-3 µl behind ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated on ice for 1 min before pelleting with a swinging-bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed and ∼50 µl were left behind to increase nuclei recovery ...
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bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for thirty minutes with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for two hours with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... This mix was incubated for 30 minutes at 37°C to form ribonucleoprotein complexes and injected into hermaphrodite adult gonads using a Transjector 5246 (Eppendorf). F1 progeny were singled out from plates displaying a high proportion of the dumpy (dpy-10 ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
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bioRxiv - Biophysics 2023Quote: ... monomer exchanges were performed by incubating a mixture of both variants for 40 minutes at 43 °C and 300 rpm in a shaking incubator (Thermomixer 5436, Eppendorf). This method was also employed to obtain Cdc371-Hsp902-fusion heterodimers (one Cdc37 per Hsp90 dimer) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was performed in a final volume of 60 μL for 60 min at 30°C under stirring (750 RPM, Thermomixer, Eppendorf). γS-ATP was removed by dialyzing the reaction mix against kinase buffer ...
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bioRxiv - Microbiology 2023Quote: ... The sample was vortexed for 30 seconds before being centrifuged for 15 mins at 4°C at 1739 x g (3000 rpm, Eppendorf 5810R benchtop centrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at 37 °C for 4 hrs under gentle orbital agitation (300 rpm on Eppendorf Thermomixer) and centrifuged at 15 000 × g for 5 min prior to SEC analysis ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the samples were 10000xg centrifuged for 10 min in a cooled centrifuge (4°C; Centrifuge 5415 R, Eppendorf, Hamburg, Germany), and the supernatant transferred to new microcentrifuge tubes ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were grown in the rich medium YEP + 2% glucose (YEPD) at 25°C with a rotation of 2000 rpm (New Brunswic Innova 43R, Eppendorf). To arrest cells in late G1 by overexpressing non-degradable Sic1(9m ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred in a new tube and the cytosolic fraction was dried under vacuum for 2 h at 55°C in a Speedvac (Eppendorf). Pellets were then stored at 4°C ...
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bioRxiv - Pathology 2023Quote: ... The samples were extracted overnight at 4℃ and then centrifuged at 4,000 rpm for 5 min at 4°C using a 5810 R fixed-angle rotor centrifuge (Eppendorf, Germany). 1 ml of the upper layer of petroleum ether was dried under vacuum in a new 2-ml tube ...
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation for 10 min at 15,000 x g and 4 °C with a 5415R benchtop microcentrifuge (Eppendorf, Hamburg, Germany), the supernatants were collected and residual sample pellets were reextracted with a 200 μL aliquot of the previously used extraction solvent mixture ...
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bioRxiv - Molecular Biology 2023Quote: ... the rehydrated gel pieces were overlaid with 30 μL of 0.01% ProteaseMAX in 50 mM ammonium bicarbonate and incubated at 37°C for 3 hours with shaking at 800 rpm (Thermomixer, Eppendorf). The digestion reaction was transferred to a fresh tube ...
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bioRxiv - Neuroscience 2023Quote: ... PFF oligomers were incubated with or without SVD-1a in PBS pH 7.4 for 3 d at 37 °C under quiescent condition in low retention Eppendorf tubes (Eppendorf, GE). Prior to injection ...
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bioRxiv - Neuroscience 2023Quote: ... After centrifuging the samples for 15 minutes at 5°C at 4000 rpm on bench top centrifuge 5810R (Eppendorf, USA), 600 μL of dichloromethane (bottom layer ...
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bioRxiv - Microbiology 2023Quote: ... cell nuclei were resuspended in 500 μl of 1.2X restriction enzyme buffer and 0.3% SDS (final concentration) and incubated at 37°C with shaking at 300 rpm (Eppendorf Thermomixer). After 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was diluted with RNAse free water to a concentration of 500ng/20ul and denatured at 70°C for 15min in a Mastercycler (Eppendorf). Mastermix was prepared by mixing ...
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bioRxiv - Neuroscience 2023Quote: Neurons remained in medium and were incubated in ∼1% O2 (5% CO2, 37°C) for 360 min in a triple-gas incubator (Eppendorf) and then returned to normoxic conditions (21% O2 ...
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bioRxiv - Cell Biology 2024Quote: ... Spheroids were incubated with primary antibodies for 24 hours at 37°C and 300 rpm shaking in a Thermomix (Eppendorf). Spheroids were rinsed with PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were sorted using a BD FACS Aria cell sorter at 4°C and collected in 0.4 mg/mL BSA in DNA LowBind tubes (Eppendorf, 0030108523). Cells were pelleted at 300 g for 5 minutes at 4°C and manually counted using a Bürker Chamber ...
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bioRxiv - Evolutionary Biology 2024Quote: 15mL of a Salten solution (1011 PFU/mL) was distributed into 1.5mL polypropylene tubes and centrifuged for 1 hour at 16,000g at 4°C (Eppendorf Centrifuge 5415R). Pellets were resuspended in 100µL of 100 mM of ammonium acetate (NH4-CH3CO2 ...
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bioRxiv - Immunology 2024Quote: ... This slurry was incubated for 1.0 h at 21 °C while shaking at 1,200 rpm on an Eppendorf shaker (Eppendorf, Hamburg, Germany). Excess of CCP4 peptide was removed by washing the beads four times with 1.0 ml PBS ...
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bioRxiv - Neuroscience 2024Quote: ... samples were layered on top of the 105 µl cushion and spun at 10,000 g for 20 min at 4°C in a swing out rotor (A-8-11 swing bucket rotor, Eppendorf) to isolate CpxII bound to trans SNARE complexes (SNAREpins ...
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bioRxiv - Neuroscience 2023Quote: ... The beads-captured proteins were digested overnight at 37 °C with 0.5 μg trypsin/LysC mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... was added and incubated for 30 min at room temperature in the dark while shaking at 1500 rpm (Thermomixer C, Eppendorf). The IAM was quenched with 11 mM DTT ...