Labshake search
Citations for Eppendorf :
651 - 700 of 911 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
-
bioRxiv - Cell Biology 2021Quote: ... Relative amount of bacteria on seeded plates was measured by washing bacteria off plates with M9 buffer following a 24 hour temperature shift to 25°C and OD600 was measured using a BioPhotomer D30 (Eppendorf).
-
bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... The reactions were carried out in a final volume of 50 μL by incubation by shaking in a Thermomixer C (Eppendorf) at 750 rpm ...
-
bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of 59.0 μM ubiquitin and 2 mM ATP then incubated at 37°C in a Mastercycler nexus (Eppendorf, Germany) thermocycler for 2 hrs unless otherwise stated ...
-
bioRxiv - Biochemistry 2020Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, Eppendorf #A-4-81 rotor) and washed with ice-cold 50 mL Binding buffer composed of 10 mM imidazole (pH 7.4) ...
-
bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
-
bioRxiv - Neuroscience 2020Quote: ... the pellet was resuspended in SK buffer (LS buffer, 1% sarkosyl, 500mM NaCl) and solubilized at 22°C for 60 minutes using a ThermoMixer 5350 (Eppendorf) at 500rpm ...
-
bioRxiv - Microbiology 2021Quote: ... The samples were immediately cooled on ice and centrifuged for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The cell pellets were stored at −20°C until RNA extraction.
-
bioRxiv - Microbiology 2021Quote: ... The cells were transferred into anaerobic 50 mL reaction tubes inside the anaerobic chamber and harvested outside of the anaerobic chamber for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The supernatant was discarded inside the anaerobic chamber and the pellet was resuspended in fresh RCM medium to an OD600 of 5-10 ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
-
bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
-
bioRxiv - Neuroscience 2019Quote: ... The lysate was cleared from the insoluble fraction by centrifugation for 15 min at 10,000 rpm (11,292 x g) at 4 °C (Eppendorf 5427 R). The supernatant was retrieved and the procedure repeated until no pellet was visible after centrifugation ...
-
bioRxiv - Biochemistry 2021Quote: ... 33 mg of MFC tissue stored at −80°C was transferred to 1.5 mL LoBind Eppendorf tubes (Eppendorf, Cat# 022431081) and combined with 0.5 mL homogenization buffer consisting of 8 M urea ...
-
bioRxiv - Biochemistry 2021Quote: ... at a molar ratio crosslinker to lysines of ∼2.7 for 30 min (except for the time-course experiments, see below) at 37°C shaking at 650 rpm in a Thermomixer (Eppendorf). Protein samples were quenched by addition of ammonium bicarbonate to a final concentration of 50 mM and either directly evaporated to dryness or after an additional centrifugation step for 60 min at 21 000 x g in order to separate the condensed from the dilute phase ...
-
bioRxiv - Biochemistry 2020Quote: ... Cultures were grown with shaking at 200 rpm at 30 °C on an Innova 2300 platform shaker (Eppendorf, Hamburg, Germany) to maximal culture density ...
-
bioRxiv - Bioengineering 2021Quote: ... a 96-well plate pre-filled with 20 μL pure water and sorted with designated cells per well was frozen on dry ice for 5 min and heated by ThermoMixer C (Eppendorf) at 95 °C for 10 min followed by spinning down at 3000 rpm for 1 min ...
-
bioRxiv - Microbiology 2020Quote: Cells were concentrated from 10 ml of the enrichment cultures by centrifugation (4500×g, 20 min, 28°C) using a swing-bucket rotor (Eppendorf) and resuspended in approximately 200 µl of the supernatant ...
-
bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
-
bioRxiv - Plant Biology 2021Quote: ... samples were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 1.0 mg per sample, pH 4.0, 60 min, 37 °C under continuous shaking in an Eppendorf thermomixer) and analysed using a UPLC-MS/MS equipped with an electrospray interface in positive mode (ACQUITY ...
-
bioRxiv - Microbiology 2021Quote: ... The dialysis block was then incubated at 37°C for 6 hours with constant shaking at 400rpm (Thermomixer comfort, Eppendorf). After 6 hours ...
-
bioRxiv - Microbiology 2021Quote: ... cells were cultured in a test tube at 30°C and optical density at 600 nm (OD600) was recorded using a spectrophotometer (Eppendorf). To obtain doubling time ...
-
bioRxiv - Systems Biology 2021Quote: ... Complementary sequences were annealed at equimolar concentrations by heating mixtures to 95 °C for 10 min in a thermal block (Eppendorf) followed by their cooling to room temperature (RT ...
-
bioRxiv - Microbiology 2019Quote: ... PMNs were sedimented for 10 min with 1000 × g at 4°C on 45° fixed-angle rotor FA-45-30-11 (Eppendorf). The supernatant was filtered by gravity through sterile PVDF 5.0 µm Millex syringe filters (Merck-Millipore) ...
-
bioRxiv - Cancer Biology 2020Quote: ... at a concentration of 100μg/ml for 1 hr at 37°C in a heat block (Thermo mixer, Eppendorf, Germany). After incubation 10μg/ml of propidium iodide was added in dark for 15 min ...
-
bioRxiv - Cell Biology 2021Quote: ... were vortexed 3 min at room temperature followed by centrifugation at 4,500 x g for 5 min at 4°C (Eppendorf #5010R). The supernatant fluid was poured into a chilled 2 ml screw-capped tube ...
-
bioRxiv - Cell Biology 2021Quote: ... The resuspended cilia sample was centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
-
bioRxiv - Biochemistry 2022Quote: ... or 1% sodium deoxycholate (SDC), incubated for 10 min at room temperature (U) or at 60°C (GnHCl, SDC) in a ThermoMixer (Eppendorf), and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode) ...
-
bioRxiv - Cell Biology 2022Quote: ... bound proteins were eluted from the streptavidin beads with 50 µl of Laemmli-DTT sample buffer containing 500 nM D-Biotin at 98°C by shaking at 1,000 rpm in a ThermoMixer (Eppendorf™). For mass spectrometry analysis ...
-
bioRxiv - Molecular Biology 2022Quote: ... Hybridization between the target mRNA and reporter-capture probe pairs was performed for 18 hours at 65°C using Mastercycler Pro S Thermal Cycler (Eppendorf) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
-
Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37°C and 5% CO2 in a humidified incubator (Galaxy 170S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
-
bioRxiv - Biophysics 2022Quote: ... with and without 1,4 dithiothreitol (DTT) (10 mM) and agitated at 400 oscillations per minute (Eppendorf Thermomixer C, Eppendorf, Germany) for 48 h.
-
bioRxiv - Bioengineering 2022Quote: ... tumors were minced using a razor and digested with 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I in PBS at 37°C for 2h with rotation at 600rpm in a thermomixer compact (Eppendorf). 10mM EDTA was then added to stop the enzymatic reaction ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37 °C and 5% CO2 in a Galaxy 170S CO2 humidified incubator (Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
-
bioRxiv - Cell Biology 2022Quote: ... then with 150 µl of 100% acetonitrile to the gel pieces for 15 min at 25°C while being agitated at 1400 rpm in a ThermoMixer (Eppendorf). Extracted peptides combined ...
-
bioRxiv - Cell Biology 2022Quote: ... The fully dried gel slices were incubated with 1 µg trypsin per tube in 50 mM ammonium bicarbonate buffer at 37°C overnight on a ThermoMixer (Eppendorf). Digested peptides were extracted twice with 150 µl of 30% acetonitrile ...
-
bioRxiv - Immunology 2020Quote: ... The cultures were placed on ice for 10 minutes to slow growth then centrifuged at 3,000 × g for 10 minutes at 4°C (Eppendorf 5810R). The supernatants were discarded and the pellets were resuspended in fresh RPMI 10mM glucose until a McFarland of 5.0 (∼109 CFU/mL ...
-
bioRxiv - Microbiology 2021Quote: ... with 100 μl aliquot of the ultra-low gelling temperature agarose gel solution (6% in Mueller Hinton Broth II, cation-adjusted) to 85 °C in a Thermomixer (Eppendorf Thermomixer R Shaker, Eppendorf). Once the temperature was reached ...
-
bioRxiv - Molecular Biology 2021Quote: ... to 10 days of storage at 40°C while shaking at 200 rpm in a thermomixer (Eppendorf Thermomixer Comfort 5355).
-
bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
-
bioRxiv - Microbiology 2020Quote: ... The 20 mL cultures used for all other analyses were incubated at 37°C in an Innova 40R shaking incubator (Eppendorf) at 230 rpm until they reached an OD600 of 0.7.
-
bioRxiv - Neuroscience 2021Quote: ... Sarkospin solubilization was then performed by incubating the samples at 37 °C under constant shaking at 600 rpm (Thermomixer, Eppendorf) for 45 min ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were digested with trypsin (1:50 trypsin:protein ratio) overnight at 37°C with light agitation (450 rpm on a Eppendorf thermomixer). Digestion was stopped by the addition of trifluoracetic acid (TFA ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Homogenates were centrifuged at 1000 rpm for 5 min at 4°C in a table centrifuge (5424 R, Eppendorf, Germany). The supernatant was transferred to a new tube and again centrifuged at 4°C for 5 min at 1400 rpm ...
-
bioRxiv - Genetics 2022Quote: ... The samples were then incubated at room temperature (about 25°C) at 450 rpm for 20 mins on Thermomixer (Eppendorf). Putting the samples on magnetic stand to facilitate removing supernatant ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 U/ml DNase I in HBSS−/−) at 37°C for 20 minutes with constant agitation (300 RPM) in a ThermoMixer (Eppendorf). All subsequent steps were performed as previously described9,14 ...
-
bioRxiv - Systems Biology 2019Quote: ... One of the fermentations was performed in CDM at 37°C in 3.0 L bioreactors (BioFlo 115, New Brunswick Scientific/Eppendorf) with a 2.2 L working volume ...
-
bioRxiv - Microbiology 2019Quote: ... proteinase K (50mg/μl) was added and the samples were incubated overnight at 56°C at 600 rpm (Thermomix comfort, Eppendorf). Appropriate amounts of AL buffer (Qiagen GmbH ...
-
bioRxiv - Molecular Biology 2020Quote: ... The obtained pellets were air-dried to rid them of residual acetone and solubilized with 120 μl Tris-SDS (10 mM Tris-HCl pH 7.5 and 2% SDS) at 56° C for 10 min and shaking at 1000 rpm in a table-top thermomixer (Eppendorf). The resulting suspension was centrifuged 20,000×g for 10 minutes at room temperature in a table-top micro-centrifuge (Eppendorf) ...