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Citations for Eppendorf :
651 - 700 of 724 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Complementary sequences were annealed at equimolar concentrations by heating mixtures to 95 °C for 10 min in a thermal block (Eppendorf) followed by their cooling to room temperature (RT ...
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bioRxiv - Microbiology 2020Quote: ... 190 μl of the lysate was combined with 10 μl of 20 mg/ml proteinase K in a PCR plate and incubated in a MasterCycler ProS (Eppendorf) for 60 min at 60°C for digestion ...
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bioRxiv - Microbiology 2021Quote: ... pre-heated to 65°C directly to pellets followed by 10 minutes of shaking at 65°C and 2000 RPM on a thermomixer (Eppendorf). RNA was extracted from the Trizol mixture using Direct-zol (Zymo ...
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bioRxiv - Biochemistry 2022Quote: ... or 1% sodium deoxycholate (SDC), incubated for 10 min at room temperature (U) or at 60°C (GnHCl, SDC) in a ThermoMixer (Eppendorf), and subsequently disrupted by 2 × 20” high intensity sonication cycles at 4°C in a BioRuptor (Diagenode) ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
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bioRxiv - Neuroscience 2022Quote: ... Serum was isolated from the clotted blood by gentle centrifugation at 1000 RPM for 10 minutes in a refrigerated centrifuge (Model 5415R, Eppendorf).
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bioRxiv - Biochemistry 2022Quote: ... with 200 μL of 3.2 mm round beads at for 10 min at setting 8 in 1.8 mL microcentrifuge vials (Eppendorf Safe-Lock). Homogenates were transferred to a new microcentrifuge vial and briefly placed on ice for phase separation before centrifugation at 14,000 × g for 15 minutes ...
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bioRxiv - Biophysics 2022Quote: ... with and without 1,4 dithiothreitol (DTT) (10 mM) and agitated at 400 oscillations per minute (Eppendorf Thermomixer C, Eppendorf, Germany) for 48 h.
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bioRxiv - Molecular Biology 2021Quote: ... to 10 days of storage at 40°C while shaking at 200 rpm in a thermomixer (Eppendorf Thermomixer Comfort 5355).
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bioRxiv - Neuroscience 2020Quote: ... or vehicle for thirteen days from postnatal day 28 (P28) to postnatal day 40 (P40) using a micropipette (0.5-10 µL; Cat. No. 3123000020; Eppendorf, Germany). Following JCNO treatment ...
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bioRxiv - Neuroscience 2020Quote: ... or vehicle for thirteen days from postnatal day 2 (P2) to postnatal day 14 (P14) using a micropipette (0.5-10 µL; Cat. No. 3123000020; Eppendorf, Germany). Mouse pups for each postnatal treatment experiment were selected from at least six different dams to minimize any litter-related bias ...
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bioRxiv - Molecular Biology 2020Quote: ... The obtained pellets were air-dried to rid them of residual acetone and solubilized with 120 μl Tris-SDS (10 mM Tris-HCl pH 7.5 and 2% SDS) at 56° C for 10 min and shaking at 1000 rpm in a table-top thermomixer (Eppendorf). The resulting suspension was centrifuged 20,000×g for 10 minutes at room temperature in a table-top micro-centrifuge (Eppendorf) ...
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bioRxiv - Microbiology 2021Quote: ... The oocysts were washed 5x by resuspending them in 10 ml of PBS and sedimenting them using a centrifuge (Eppendorf 5810R ...
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bioRxiv - Biophysics 2021Quote: ... and the aggregated samples were pelleted via centrifugation at 20.000 g for 10 min (Eppendorf 5417 R table-top centrifuge). The pellets were washed with 500 μL ice-cold acetone and centrifuged for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... were diluted in water (1:10) and injected into the periplasm of the embryos during cellularization using a FemtoJet express microinjector (Eppendorf). The embryos used in this experiment expressed Utr-Venus and Sqh-mCherry which allowed us to monitor cell shape and actomyosin dynamics ...
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bioRxiv - Biochemistry 2022Quote: ... These samples were reduced by addition of dithiothreitol (DTT) to 10 mM final concentration and incubation at 37 °C with agitation (700 rpm) in a benchtop ThermoMixer (Eppendorf) for 45 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting pellet was re-suspended in 5 mL of modified CIB medium and centrifuged at 16,000 gav for 10 min in 5415R refrigerated centrifuge (Eppendorf, USA). The resulting supernatant was the Sucrose Gradient Load (SGL) ...
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bioRxiv - Microbiology 2022Quote: ... The filter centrifuge tubes containing the samples were centrifuged for 10 minutes at 12000 rpm (Eppendorf, 5424 R, 13523 ×g). After centrifugation ...
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bioRxiv - Neuroscience 2022Quote: ... Particles were collected by centrifugation at 1000 xg for 10 minuntes (Eppendorf centrifuge 5864 R, Eppendorf North America, Hauppauge, NY) and washed three times by discarding the supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... and streptomycin (10 µg ml−1) at 37° C for 40 min with shaking at 800 rpm in a thermomixer (Eppendorf). Digested medullae were resuspended and the enzymatic reaction quenched by addition of 10 volumes cold CMF-HBSS ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cell suspension was then transferred to a pre-chilled electroporation cuvette and after approximately 5 – 10 min electroporated in the eporator electroporator (Eppendorf) set to 1850 V ...
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bioRxiv - Genomics 2024Quote: ... containing 0.25% SDS was used to thoroughly resuspend and permeabilize the nuclei and incubated at 62°C for 10 min using Eppendorf Thermomixer C (Eppendorf). After the incubation ...
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bioRxiv - Bioengineering 2024Quote: ... the gel was further degassed using customized 10 mL syringes (Becton, Dickinson, and Company) with a syringe filter top centrifuged (5702 R, Eppendorf) for five minutes at 2500 rpm ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Aliquots of 1.5 OD600 were made for each strain and pre-incubated at their restrictive temperatures for 10 min at 800 rpm in a Thermoblock (Eppendorf). Proteins were labelled for 5 mins (for soluble proteins ...
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bioRxiv - Neuroscience 2023Quote: ... To make 10 μl of 20 μM primer pairs per each gene in V-shaped 96 well plate (Eppendorf, #951020401), 4 μl of forward and reverse primers were added to 2 μl H2O.
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bioRxiv - Molecular Biology 2023Quote: ... The proteins that adhered to the filter were then incubated with 200 µL of 10 mM dithiothreitol in lysis buffer using a ThermoMixer (Eppendorf) at 37°C and 600 rpm for 1 min and then with no agitation for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 150 µL of refreshed mid-log phase cultures and 0.2 µg of MTS-mCherry-GFP1-10-clonNat DNA were used in the transformation setup on the epMotion 5075 liquid handling workstation (Eppendorf). To optimize transformation efficiency ...
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bioRxiv - Microbiology 2024Quote: The 2000 mL liquid cultures were transferred to sterile 500 mL centrifuge containers and centrifuged at 3214 g for 10 minutes at room temperature (Eppendorf, model 5810 R ...
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bioRxiv - Microbiology 2024Quote: ... Aqueous phage was separated by centrifugation (14,000 rpm for 10 minutes at room temperature in 5418 R Centrifuge equipped with FA-45-18-11 rotor (Eppendorf)) ...
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bioRxiv - Plant Biology 2024Quote: ... Digests were then extracted with 750 μL of 25:24:1 phenol-chloroform-isoamyl alcohol pH 8 (Fisher #BP17521-400) and centrifuged at maximum speed for 10 minutes in a tabletop centrifuge (Eppendorf). The aqueous phase was transferred to a new 1.5 mL eppendorf tubes ...
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bioRxiv - Plant Biology 2024Quote: The wheat flour (10 mg) from nine cultivars was weighed into 1.5 mL low protein bind tubes (Eppendorf, Hamburg, Germany) in four replicates ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were then centrifuged at 14,000xg for 10 minutes to pellet insoluble debris and the resultant clarified lysate was transferred to a fresh protein LoBind tube (Eppendorf).
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bioRxiv - Systems Biology 2024Quote: ... for 10-14 days and collected in 0.1 gramme fresh weight (gFW) aliquots in 2ml microcentrifuge tubes (Safelock®, Eppendorf), along with two ...
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Scalable Fabrication of a Tough and Recyclable Spore-Bearing Biocomposite Thermoplastic PolyurethanebioRxiv - Bioengineering 2024Quote: ... Spores were further washed with PBS by repeating the following steps for three times: (i) centrifugation at 2200 g at 25 °C for 10 min (5810 R, Eppendorf), (ii ...
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bioRxiv - Biophysics 2024Quote: 50 or 100 uM PDZ was incubated with 50 uM acetylated α-Syn fibril in 10 mM PBS (pH 7.4) at 37°C 300 rpm for 24 hours (Thermomixer C, Eppendorf, MA). 50 uM PDZ only and 50 uM acetylated α-Syn fibril only samples with the same treatment were used as controls ...
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bioRxiv - Biophysics 2024Quote: ... 50 μM non-acetylated α-Syn was incubated in the absence and presence of 50 μM HtrA1* in 10 mM PBS (pH 7.4) at 37°C 900 rpm for one week in a thermomixer (Thermomixer C, Eppendorf, MA). After one week of fibrilization ...
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bioRxiv - Biophysics 2024Quote: ... syMagIcS were microinjected into cells based on published protocols54 using a micro manipulation system (InjectMan NI2 and 10 FemtoJet Express, Eppendorf) and a capillary pressure between 15-25 hPa ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested by suspending the washed tips in 100 μL lysis buffer and were heated at 95 °C for 10 mins with agitation (Thermomixer, Eppendorf). After heating ...
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bioRxiv - Cell Biology 2024Quote: ... a mix of pre-polymerized mother filaments (formed from 1 μM 10 % AlexaFluor-568-labeled G-actin incubated in F-buffer in an Eppendorf tube for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Unreacted dye was removed by two passages over an equilibrated Bio-spin® 6 column filled with Bio-gel P-6 media in labeling buffer and centrifuged in a tabletop centrifuge (Eppendorf 5810 R, rotor A-4-62) at 1500 rpm for 3 min ...
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bioRxiv - Cell Biology 2020Quote: ... The cell suspension was centrifuged at full speed (7,197 × g) at 20 °C for 10 minutes in a refrigerated centrifuge (cat. 5430 R, Eppendorf, Hauppauge, NY). The supernatant was discarded ...
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bioRxiv - Cell Biology 2021Quote: ... lipid extracts were dissolved in 10 mM ammonium acetate in methanol and transferred to 96-well plates (Eppendorf twintec plate 96). Mass spectrometric measurements were performed in positive ion mode on an AB SCIEX QTRAP 6500+ mass spectrometer ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 230,000g for 10 min at 25 ◻ using CS100GXL ultracentrifuge and S120AT3 rotor (Eppendorf Himac Technologies) and resultant supernatant and pellet were analyzed by SDS-PAGE followed by Coomasie Brilliant Blue R-250 staining.
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bioRxiv - Evolutionary Biology 2020Quote: ... The sample was incubated at 30 °C until visible cell lysis had occurred and subsequently centrifuged at 3,500 x g for 10 min in an Eppendorf tabletop centrifuge 5804R (Eppendorf, Hamburg, Germany) to eliminate cell debris ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted in BME (1:10) and locally injected into the outer SVZ by using a FemtoJet® 4i microinjector (Eppendorf). After 72 h ...
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bioRxiv - Microbiology 2022Quote: ... Extracts were centrifuged twice at 1,000 rpm (180 x g) for 10 minutes in an Eppendorf 5810R centrifuge (Eppendorf, Hauppauge, NY) to remove gross rabbit cellular debris ...
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bioRxiv - Genomics 2024Quote: ... The grown bacterial cells were subsequently centrifuged at 5000 G for 10 min using a 5920 R centrifuge (Eppendorf, Hamburg, Germany) washed in 1 mL 0.9% NaCl and subsequently centrifuged again at 12000 rpm for 3 min ...
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bioRxiv - Microbiology 2024Quote: ... Sampling was performed at intervals of 6 to 14 hours and the OD600 was measured in 10 mm cuvettes using a BioSpectrometer (Eppendorf AG). Supernatants were centrifuged at 12,000 rpm (Thermo Scientific™ Fresco™ 21 Microcentrifuge ...
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bioRxiv - Microbiology 2023Quote: ... culture samples (10 ml) were centrifuged at 5000 rpm in a F-34-6-38 rotor in a 5804R centrifuge (Eppendorf AG) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... was adjusted to 50 ng/μL with 50% ACN containing 0.1% TFA and dispensed in 10 μL each into normal tubes (1.5-mL safe-lock tube, CAT# 0030120086, Eppendorf, Hamburg; Germany), normal polypropylene (PP ...