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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: MirVana™ miR-212-3p Mimic and scramble negative control were purchased from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and rno-miR-674-3p (RT:001956, ThermoFisher Scientific). Data were analyzed using StepOne Software v ...
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bioRxiv - Neuroscience 2022Quote: ... We used reverse-transcriptase-specific primers for mmu-miR-134 (Applied Biosystems miRNA assay ID 001186 ...
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bioRxiv - Neuroscience 2024Quote: ... we used rno-miR-191a-3p (RT:002576, ThermoFisher Scientific), which was selected as the most stable in our experiment ...
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bioRxiv - Cell Biology 2021Quote: ... miR-30a-3p (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and mmu-miR293-3p (ID 001794) TaqMan MicroRNA Assays (ThermoFisher). The final volume of 15 µL for each reaction underwent reverse transcription using a BioRad T100 Thermal Cycler at 16 °C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse-transcriptase-specific primers for mmu-miR-335-5p (Applied Biosystems miRNA assay ID 000546) were used and real-time quantitative PCR performed using TaqMan miRNA assays (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... with specific miR inhibitors (MH10203 for hsa-miR-22-3p and MH13382 for hsa-miR-1220-3p; Thermo Scientific) using Lipofectamine RNAiMax ...
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bioRxiv - Bioengineering 2023Quote: Macrophages expressing the NGL NF-κB-luciferase reporter were either pre-treated with iEVs at a dose corresponding to an iEV donor and recipient cell ratio of 20:122 or transfected with a mirVana miRNA mimic mmu-miR-147-3p (Thermo Fisher Scientific) with Lipofectamine™ RNAiMAX Transfection Reagent (Life technologies ...
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bioRxiv - Cancer Biology 2021Quote: MiR-212-3p expression was quantified using TaqMan™ MicroRNA Reverse Transcription Kit and TaqMan™ Advanced miRNA Assay (Applied Biosystems). Isolated total RNA was reverse transcribed using a specific stem-loop primer for miR-212-3p and RNA U6B ...
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bioRxiv - Genetics 2020Quote: ... hsa-miR-147b-3p and hsa-miR-210-3p levels were assessed by TaqMan Advanced miRNA Assays (Thermofisher) and TaqMan Fast Advanced miRNA master mix (Thermofisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... miR-320a-3p (Thermo Fisher Scientific, 478594_mir) was used as endogenous control ...
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bioRxiv - Pathology 2020Quote: ... Assay ID 245306_mat; 4427975, hsa-mir-23a-3p, Assay ID 000399; 4427975, cel-miR-39-3p, Assay ID 000200, Thermofisher). The relative expression of individual miRNA was calculated as ΔCt = mean Ct miRNA-mean Ct miR-39.
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bioRxiv - Cancer Biology 2021Quote: ... Q-RT-PCR was performed using TaqMan primers/probe sets (Applied Biosystems) and analyzed as transcript copies per 50 ng RNA expressed relative to RNAPII expression as previously described (33).
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bioRxiv - Cell Biology 2020Quote: ... TaqMan Fast Universal PCR Mastermix and TaqMan miRNA Assays primers were used to detect miR-23a-3p and miR-10b-5p (QuantStudio 12K Flex, Thermo Fisher Scientific). All reactions were run in triplicate and results were expressed as 2−ΔΔCt ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ...
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bioRxiv - Molecular Biology 2020Quote: ... miR-200a-3p (MC10991) and negative control miRNA (Ambion) were used for these transfections.
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using Oligofectamine (Invitrogen). Firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... miRNA mimics and the miR-100 isoforms miR-100-5p and miR-100-3p (all from Life Technologies) using 15 pmol PepMute reagent (SignaGen Labs ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with hsa-miR-3p mimic (Invitrogen) or a control mimic at 5 nM final concentration using Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... or hsa-miR-106b-3p mirVana™ miRNA Mimic (Ambion) at final concentration of 5 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using (Lipofectamine RNAiMAX, Life Technologies). Twenty-four hours after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... We also measured miR-375-3p (Thermo Fisher Assay ID #00564), which was not included on the array ...
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bioRxiv - Bioengineering 2023Quote: ... synthetic cel-miR-39-3p (Thermo Fisher Scientific, Waltham, MA, USA) was added to samples during the cell/MP lysis step as a spike-in control ...
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bioRxiv - Immunology 2021Quote: ... Taqman miRNA assays for the housekeeping control gene U6 snRNA (#001973) and hsa-miR-21-5p (which also recognizes mmu-miR-21a-5p) (#000397) were purchased from Thermo Fisher. qPCR was performed using Taqman Universal Master Mix II ...
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bioRxiv - Physiology 2020Quote: ... The multiplex RT primer pool consisted of primers for miR-150-5p and U6 (Thermo Fisher Scientific). No-template samples where included as negative controls.
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bioRxiv - Neuroscience 2021Quote: ... or miR-544-3p inhibitor (50 nM; Thermo Fisher Scientific, Waltham, MA), or negative control mimic (50 nM ...
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p inhibitor (MIMAT002816) (Cat 4464084, Assay ID MH12409, Ambion). Negative Control LNA mimic (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p mimic (MIMAT002816) (Cat 4404066, Assay ID MC12409, Ambion), mirVana miR inhibitor negative control #1 (Cat 4464076 ...
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bioRxiv - Genomics 2023Quote: ... TaqMan™ MicroRNA Assay hsa-miR-124-3p (ThermoFisher, AssayID 003188_mat, 4440886), TaqMan™ MicroRNA Control Assay U6 snRNA (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... 0.5 pmol synthetic cel-miR-39-3p (Thermo Scientific, Waltham, MA, USA) was added to the samples during cell/MP lysis as a spike-in control ...
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bioRxiv - Microbiology 2020Quote: ... 18S rRNA endogenous control primer/probe set was utilized for semi-quantitative RT-PCR normalization (ThermoFisher, 4333760T). Thermal cycling conditions were adapted from Verso 1-step RT-qPCR kit (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... The probe sequences for mmu-miR-129-5p and U6 snRNA were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 U Ribolock (40U/μl), 1x TaqMan RT primer per each gene of interest (miR-7a, miR-671, Let-7a, snoRNA202, U6 snRNA) (Applied Biosystems) and 1x first-strand synthesis buffer were filled up to 20 μl reaction volume with ddH2O and mixed ...
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bioRxiv - Molecular Biology 2020Quote: ... or miR-324-3p.1 along with control miRNA or miR-324 duplexes using the Lipofectamine 3000 reagent (Thermo Scientific). After 2 days of transfection ...
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bioRxiv - Immunology 2020Quote: ... Probes for TaqManTM MicroRNA Assay (hsa-miR-424-3p, hsa-miR-424-5p, U6 snRNA control) were purchased from ThermoFisher. All the steps were performed according to manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: ... PCR primers and probe sets were obtained from Thermofisher, USA ...
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bioRxiv - Neuroscience 2021Quote: ... and DRG neurons were then electroporated with miR-544-3p mimic (50 nM; Thermofisher), or miR-544-3p inhibitor (50 nM ...
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Unannotated small RNA clusters in circulating extracellular vesicles detect early stage liver cancerbioRxiv - Cancer Biology 2020Quote: ... and purchased a catalog TaqMan® miRNA Assay against cel-miR-39-3p (ThermoFisher) to target the spike-in miRNA mimic which was used during the exRNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... miR-504-3p or miR-574-5p were reverse transcribed by using TaqMan™ Advanced miRNA cDNA Synthesis Kit (Thermo Scientific) and then amplified by using specific primers provided in the TaqMan Advanced miRNA Assays (Thermo Scientific ...
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bioRxiv - Pathology 2020Quote: ... 100 ng of wild-type or mutant reporter vectors were co-transfected with 20 nM miRNA (miR-574-5p or miR-574-3p) into HEK293T cells cultured in 24-well plate using lipofectamine 3000 (ThermoFisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... Two sets of primers were used: Megaplex RT Primers Human pool A and B (Thermo Fisher, USA). Together ...
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bioRxiv - Cell Biology 2020Quote: ... the mmu-miR-762 mimic and inhibitor were mixed with LipofectamineTM 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA), incubated for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... PLGA nanoparticles incorporating a mirVana miRNA mimic hsa-mir 132-3p (4464066, Thermo Fisher Science) were prepared using an emulsion solvent diffusion method in RNAase-free water ...
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bioRxiv - Cancer Biology 2019Quote: 50-100ng of genomic DNA from edited cells in a total PCR reaction volume of 50ul were amplified in a 30 cycle PCR using a locus-specific primer set (primer set 1) and AmpliTaq Gold 360 Master Mix (Applied Biosystems) (Supplemental Table 1b) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1-2μl of extracted genomic DNA in a total PCR reaction volume of 25μl were amplified in a 35 cycle PCR using a locus-specific primer set (primer set 1) and AmpliTaq Gold 360 Master Mix (Applied Biosystems) (Supplemental Table 1b) ...
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bioRxiv - Cancer Biology 2021Quote: ... with miR-92a-specific primers (Applied Biosystems). Quantitative PCR was performed using FAST SYBR green mix (Roche ...
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bioRxiv - Plant Biology 2022Quote: Custom TaqMan® probes (including RT primers and qRT-PCR primers, Thermo Scientific) were designed using the Small RNA Assay Design Tool with the following input sequences ...