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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-SARS-CoV-2 S antibody (PA5-81795, Invitrogen, 1:1000); rabbit anti-TDRD3 antibody (Bethyl Laboratory ...
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bioRxiv - Microbiology 2020Quote: ... anti-SARS CoV-2 nucleocapsid mouse monoclonal (1:10) (Thermo Fisher Scientific) and anti-ACE2 mouse monoclonal (1:20 ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with 400 ng/well 2-E plasmids or 2-E mutations using Lipofectamine 3000 Transfection Reagent (L3000015, Thermo Fisher, MA, USA). The control group was Vero E6 cells transfected with 400 ng/well pcDNA3.1 vector plasmids alone ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Developmental Biology 2023Quote: ... media was changed to Hepatocyte Medium E (William’s E Medium [Gibco] ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 S protein Omicron genes were synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific). Pseudotyped retroviral particles were produced in HEK293T cells as described previously (32) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transiently transfected with 2μg of either wild-type or mutant SARS-CoV-2 S protein plasmid with Lipofectamine 3000 (Invitrogen) at a ratio of 1:2:2 DNA ...
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bioRxiv - Microbiology 2023Quote: ... and the insect cell codon-optimized RBD at residues 330 to 521 of SARS-CoV-2 S protein (GenBank accession no. MN908947.3) was cloned into a pFastBac plasmid (Invitrogen). For the RBD-LTA fusion protein expression ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were transformed into chemocompetent bacteria (E. coli Top10™, Thermo Fisher Scientific, TFS), which were generated like previously described70 ...
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bioRxiv - Bioengineering 2023Quote: MMP9-pro-cat-Hiswt (E coli expression) was expressed in BL21(DE3) (Thermo Fisher). Cells were grown in 2xYT at 37°C and expression was induced with 0.5 mM isopropyl ?-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Genomics 2019Quote: ... size selection of the libraries was performed using an E-gel Safe Imager and 2% E-gel size select gels (Invitrogen). Indexed libraries were pooled and 100 bp paired-end sequenced on the same flow cell of an Illumina HiSeq4000 instrument at the Berkeley sequencing facility ...
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bioRxiv - Biochemistry 2022Quote: ... DNA was analyzed using an E-Gel Power SNAP system on a 2% E-Gel Ex gel (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... and 2 mM Compound E onto polyethylenimine (PEI) (Thermofisher, AC178571000) and laminin-coated 24-well CytoView MEA plates (Axion Biosystems ...
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bioRxiv - Genomics 2020Quote: ... visualization on a 2% agarose E-Gel (Invitrogen, Carlsbad, CA), and quantification using picogreen ...
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bioRxiv - Bioengineering 2020Quote: ... and 2% agarose gels (E-Gel, #G501802, Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2019Quote: ... rat anti-E-Cadherin (Invitrogen clone ECCD-2, 1:500), rabbit anti-PH3 (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... and samples were visualised on 2% SYBR E-gel (ThermoFisher). A secondary PCR to index the amplicons was performed with the same polymerase master mix ...
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bioRxiv - Microbiology 2020Quote: cDNA of SARS-CoV-2 was made using SuperScript IV Reverse Transcriptase (Invitrogen) following manufacturer’s instructions with modifications ...
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bioRxiv - Biochemistry 2021Quote: ... and 1:4 pD614G-SARS-CoV-2-spike:pPyEGFP using Lipofectamine 2000 (Life Technologies), and media was replaced on day 1 ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 stocks were titrated on Vero E6 cells using MEM (Gibco), 2% FCS (Labtech ...
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared using Ion AmpliSeq SARS-CoV-2 Kit (Thermo Scientific, USA) following the manufacturer’s Protocol ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 S genes using Lipofectamine 3000 transfection reagent (ThermoFisher, CA)15 ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin was visualized with rat anti-E-cadherin (Invitrogen #13-1900), both at 1:100 concentrations as per manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Microbiology 2022Quote: ... 1:5,000 dilution) (rabbit anti-Calreticulin, Cell Signaling, Cat #: 12238, 1:2,000 dilution) (mouse anti-Spike S2 or SARS-CoV Spike, Thermo Fisher, Cat #: MA5-35946, 1:2,000 dilution) (rabbit anti-anti-Spike S2 ...
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bioRxiv - Cell Biology 2022Quote: ... worms were then transferred to 10 cm NGM conditioning plates (seeded with OP50 E. coli bacteria and with 12 μl 10% 2-butanone (Acros Organics) in ethanol streaked across the lid with a pipette tip for 1 h ...
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bioRxiv - Genetics 2023Quote: ... worms were then transferred to 10 cm NGM conditioning plates (seeded with OP50 E. coli bacteria and with 18 mL 10% 2-butanone (Acros Organics) dissolved in ethanol on the lid ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin (Invitrogen ECCD2) and GAPDH (Thermo Fisher MA5-15738 ...
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bioRxiv - Physiology 2023Quote: ... E (Life Technologies, USA). Each resulting barcoded library was quantified and assessed for quality using the Agilent Bioanalyzer ...
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bioRxiv - Developmental Biology 2024Quote: ... E-CAD (Invitrogen, 14324980), FOXP2 (1:250 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We selected libraries within the size range of 450-500 bp using a 2% E-gel on an E-Gel Electrophoresis System (Invitrogen, Canada), and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... the libraries were checked for correct fragment length on an E-Base device using E-Gel 96 Gels with 2% mSYBR Safe DNA Gel Stain (Fisher Scientific), quantified with a QuantiFluor dsDNA System (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 S.dCT were co-transfected into HEK293T cells using lipofectamine 2000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S and RBD proteins were transiently expressed in Freestyle 293F system (ThermoFisher). In brief ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... and S protein expressing pcDNA3.1-SARS CoV-2 SΔCT were co-transfected into HEK293T cells by lipofectamine 2000 (Thermo Fisher Scientific). The supernatants containing the pseudotype viruses were collected 48 h after transfection ...
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bioRxiv - Microbiology 2022Quote: ... BHK-21 were transfected to express the SARS-CoV-2 S protein using Lipofectamine 3000 (Thermo Fisher Scientific, Madrid, Spain) and after 24h transfected cells were inoculated with a replication-deficient rVSV-luc pseudotype (MOI ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... They were then co-transfected with 500 ng of IRF3-GFP and 500 ng of SARS-CoV-2 protein-expressing plasmid using Lipofectamine 2000 reagent (11668019, Invitrogen). 24 hours post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Immunology 2023Quote: ... and stained for SARS-CoV-2 N protein using Biotin-labelled-CR3009 antibody produced in-house together with a Streptavidin-Alexa488 (S32354, Invitrogen) and cellular DNA using DAPI (10236276001 ...
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bioRxiv - Microbiology 2022Quote: ... Transformations were performed in Top10 competent bacteria (One Shot TOP10 chemically competent E. coli, Invitrogen) and selected using 100 µg/mL ampicillin or/and 34 µg/mL chloramphenicol (Euromedex) ...
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bioRxiv - Molecular Biology 2019Quote: ... polyA-tailed using Escherichia coli poly(A) Polymerase (E-PAP) and ATP (Thermo Fisher Scientific), and recovered using a MEGAclear kit (Thermo Fisher Scientific ...