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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli strain DH5α (E. coli DH5α, Thermo Fisher Scientific, Cat#18258012), E ...
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bioRxiv - Immunology 2022Quote: ... and the QI9 peptide (QYIKWPWYI, residues 1208-1216 of the SARS-CoV-2 spike protein) maintained in RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS and 30 U/ml recombinant human IL-2 (Peprotec ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with 2.5 µg of a SARS-CoV spike protein polyclonal antibody (PA1-41165, Thermo Fisher Scientific) for 30 min on ice before washing with 2.5 mL of FACS buffer and centrifuging at 1750 RPM for 7 min ...
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bioRxiv - Immunology 2021Quote: ... blots were incubated with an anti-SARS-CoV spike protein polyclonal antibodies (S1, Sino Biological #40591-T62; S2, Invitrogen #PA1-41165 ...
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bioRxiv - Microbiology 2023Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... NGS was performed using primer sets that tiled the entire SARS-CoV-2 genome (Ion AmpliSeq SARS-CoV-2 Research Panel, ThermoFisher), with library preparation and sequencing performed on Ion Chef and S5 ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike gene was amplified by PCR using Phusion Hot Start II DNA polymerase (Thermo Scientific™) and a set of primers recognizing sequences upstream and downstream of the spike gene (Forward ...
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bioRxiv - Microbiology 2020Quote: ... anti-SARS-CoV-2 S (Invitrogen, PA1-41165); anti-SARS-CoV-2 Nucleoprotein (ABIN129544) ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S protein was conjugated to streptavidin-AF488 (Life Technologies #S11223), the HCoV-HKU1 S protein to streptavidin-BV421 (BD Pharmingen #563259 ...
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bioRxiv - Bioengineering 2021Quote: ... and 2.5 μg of SARS-CoV-2-d18 spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Microbiology 2022Quote: Hybridoma Screening: Expi293F cells were transiently transfected to express SARS-CoV-2 spike (Wuhan) using Lipofectamine 3000 (L3000001, Thermo Fisher) and then incubated with supernatant from the hybridoma cell lines from each fusion ...
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bioRxiv - Immunology 2022Quote: ... transfected HEK293T cells were stained with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) and an Alexa Fluor 488-conjugated goat anti-rabbit IgG (A11034, Invitrogen). Cells were then acquired on a BD Accuri C6 plus and analyzed by FlowJo software version 10.6.2.
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bioRxiv - Immunology 2021Quote: HEK293T cells were transiently transfected with plasmids encoding full length SARS-CoV-2 spike variants using lipofectamine 3000 (L3000-001, ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: HEK293T cells were transiently transfected with plasmids encoding full length SARS-CoV-2 spike variants using lipofectamine 3000 (L3000-001, ThermoFisher) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.09 μL Ambion RNase H (E. coli) (Invitrogen, AM2293). Second strand synthesis was performed by incubating samples at 16°C for 150 min ...
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bioRxiv - Biochemistry 2021Quote: ... and TnsB (WP_084763316.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Immunology 2021Quote: ... pcDNA3.1-SARS CoV-2 SΔCT using lipofectamine 2000 (ThermoFisher). This approach was used to generate pseudoviruses specific to SARS-CoV-2 variant strains ...
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bioRxiv - Microbiology 2021Quote: ... antibodies targeting the SARS-CoV-2 nucleoprotein (N) (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-S (S1) (PA5-81795, Thermo Fisher); TMPRSS2 (sc-515727 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 nucleocapsid (Invitrogen #PA5-81794; 1:1000), Spike (CST #56996S ...
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bioRxiv - Microbiology 2022Quote: ... Expression of E was detected using an HA-tag antibody (Invitrogen). β-actin was used as a loading control.
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bioRxiv - Immunology 2021Quote: ... all PE labelled and prefusion SARS-CoV-2 S-Avi tag conjugated with streptavidin Alexa-Fluor 647 (Life Technologies). SARS-CoV-2-specific IgG+ memory B cells were sorted and seeded on MSC (mesenchymal stromal cells ...
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bioRxiv - Microbiology 2021Quote: ... and prefusion SARS-CoV-2 S with a biotinylated Avi-tag conjugated to Streptavidin Alexa-Fluor 647 (Life Technologies). SARS-CoV-2 S-specific IgG+ memory B cells were sorted by flow cytometry via gating for PE negative and Alexa-Fluor 647 positive cells ...
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bioRxiv - Immunology 2021Quote: ... and prefusion SARS-CoV-2 S with a biotinylated avi tag conjugated to Streptavidin Alexa-Fluor 647 (Life Technologies). SARSCoV-2 S-specific IgG+ memory B cells were sorted by flow cytometry via gating for PE negative and Alexa-Fluor 647 positive cells ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 and SARS-CoV were expressed in Hi5 cells using the Bac-to-Bac baculovirus system (Invitrogen). Briefly ...
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Microbiology 2020Quote: ... the purified SARS-CoV-2 RBD protein was coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at around 5μg/mL in PBS overnight ...
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bioRxiv - Microbiology 2021Quote: ... the purified SARS-CoV-2 RBD proteins were coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at 5μg/mL in PBS overnight ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Microbiology 2021Quote: A codon optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2022Quote: A codon-optimized version of the reference Wuhan SARS-CoV-2 Spike (GenBank: QHD43416.1) was ordered as a synthetic gene (GeneArt, Thermo Fisher Scientific) and was cloned into a phCMV backbone (GeneBank ...
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bioRxiv - Microbiology 2021Quote: Point mutations to generate the mutants were introduced by site-directed mutagenesis of phCMV-SARS-CoV-2-Spike using Q5 polymerase (Thermo Scientific) and validated by sanger sequencing ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...
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bioRxiv - Immunology 2021Quote: ... and the SARS-CoV-2 spike gene was reverse-transcribed and amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
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bioRxiv - Immunology 2020Quote: ... The cells were blocked with 5 % skim milk-PBS for 1 hour at RT and incubated with an antibody against SARS-CoV-2 spike (BLSN-005P, Beta Lifescience) or control IgG (Thermo Fisher) at 4 °C overnight ...
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bioRxiv - Genomics 2023Quote: ... The trimeric RhGB07 spike protein was expressed as previously reported as for the SARS-CoV-2 spike transiently expressed in suspension-adapted ExpiCHO cells (Thermo Fisher) in ProCHO5 medium (Lonza ...
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bioRxiv - Biochemistry 2022Quote: ... and Escherichia coli S15 (AP009048.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
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bioRxiv - Microbiology 2022Quote: ... pylori NAP proteins (SARS-CoV-2S6p3 and SARS-CoV-2S6p312, respectively) were produced upon transient expression in Expi293F cells (ThermoFisher). Clarified supernatants were purified by affinity chromatography using an anti-HA affinity matrix (Cat# 11 815 016 001 ...
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bioRxiv - Developmental Biology 2022Quote: ... E-cadherin (rat, Life Technologies ECCD-2), Podoplanin (hamster ...
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bioRxiv - Immunology 2022Quote: ... loaded on 2% E-Gel (Thermo Fisher), and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Microbiology 2022Quote: ... and stained using SARS/SARS-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody (Invitrogen MA5-29981) as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies of anti-SARS/SARS-CoV-2 Nucleocapsid Monoclonal Antibody (Invitrogen, MA1-7404) is diluted in section staining buffer at 1:400 and sections are stained at 4°C overnight ...
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bioRxiv - Biophysics 2020Quote: CLGltPh and double cysteine mutants were transformed into Escherichia coli (E. coli) Top10 cells (Invitrogen). Bacterial cells were inoculated in Luria Broth medium containing 100 μg/mL ampicillin at 37 °C until OD600 ~0.6-0.8 ...
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Molecular Biology 2020Quote: Artificial codon optimized genes encoding SARS-CoV-2 nsp10 and nsp16 proteins were commercially synthesized (Invitrogen) and cloned in a pSUMO vector that encodes an N-terminal His8x-SUMO tag ...