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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...
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bioRxiv - Immunology 2020Quote: The prefusion ectodomain of the SARS-CoV-2 Spike protein was transiently transfected into suspension-adapted ExpiCHO cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Immunology 2021Quote: ... with SARS-Cov-2 spike plasmid or a control plasmid using Lipofectamine 2000 (Life technologies) are kind gifts from Olivier Schwartz ...
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA (pVAX1 or pVAX1-SARS-CoV-2 Spike) was transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RBD from SARS-CoV and SARS-CoV-2 were biotinylated (Thermo Fisher) and immobilized on Streptavidin (SA ...
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bioRxiv - Bioengineering 2019Quote: ... fluorescent Escherichia coli (E. coli) BioParticles (ThermoFisher, cat. #E2863), or fluorescent carboxylate-modified 1 µm polystyrene beads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... E and M proteins were resuspended using trypsin (Thermo Fisher Scientific) (1 in 5 dilution in PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... and transfected with 24 µg of plasmid encoding either the wildtype or E406W SARS-CoV-2 spike protein using Lipofectamine 2000 (Life Technologies). Four hours at transfection ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Microbiology 2021Quote: ... The positive control was addition of 10 μg/mL of SARS-CoV-2 spike protein antibody (pAb, T01KHuRb) (ThermoFisher, MA, USA) at the time of infection.
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Immunology 2022Quote: ... Sections were then incubated with cocktails of primary antibodies: rabbit anti-SARS-CoV-2 Spike Protein (1:100, Invitrogen, #MA5-36087) + rat anti-ERα H222 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 5% FBS and 0.5% Triton X-100) for 30 minutes and washed before incubation with an anti-SARS-CoV-2 spike protein antibody (Life Technologies). Nuclei and F-actin were stained using DAPI and Phalloidin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 virus was labelled using first an anti-SARS-CoV-2 antibody (Spike protein, Thermo Fischer) and then a secondary anti-rabbit Alexa 647 (Thermo Fisher).
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... Bound SARS-CoV-2 Spike was detected by antiLJHis probe HRP (Cat#15165; Thermo Fisher Scientific). Some wells received no antibody (no antibody control) ...
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bioRxiv - Microbiology 2022Quote: All SARS-CoV-2 Spike constructs for pseudotype generation were expressed from plasmid pCDNA3.1 (ThermoFisher #V79020). Codon optimized SARS-CoV-2 Wuhan Spike carrying the D614G amino acid change (Sino Biological #VG40589-UT(D614G) ...
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bioRxiv - Molecular Biology 2024Quote: ... coli poly(A) polymerase (E-PAP, Invitrogen) at 37°C for 30 min in 50 µL reactions ...
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bioRxiv - Microbiology 2024Quote: ... To transform Stbl3 E coli cells (Invitrogen) with the BAC construct ...
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bioRxiv - Microbiology 2024Quote: ... To visualize spike in Western Blots we used anti SARS-CoV-2 Spike S2 (1A9) mouse monoclonal antibody (ThermoFisher, MA5-35946) at 1:1000 diluted in 3%BSA in TBS-T (0.01% Tween) ...
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bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science ...
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bioRxiv - Developmental Biology 2019Quote: ... 2% (E-Gel, GIBCO). DNA fragments from the complete and size-selected library were detected and confirmed ...
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bioRxiv - Immunology 2021Quote: ... HIV-based lentiviral pseudotyped particles harboring the SARS-CoV-2 Spike protein variants were produced using the ViraPower Lentiviral Expression system (Thermo Fisher Scientific) according to manufacturer’s protocol with minor changes ...
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bioRxiv - Immunology 2023Quote: ... The cells were then transfected with ancestral or variant SARS-CoV-2 spike protein encoding plasmid using Lipofectamine 3000 Transfection Reagent (L3000001; Thermo Fisher Scientific). After 24 hours ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse SARS-CoV-2-spike (GTX, 1:100) and anti-rabbit hACE2 (Thermo Fisher,1:100) were applied overnight at 4 °C followed by incubation of appropriate secondary antibodies conjugated with fluorophores ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2021Quote: ... transfected with 5 μg of an expression plasmid coding either VSV-G (pMD.G) or SARS-CoV-2 Spike del19 (pcDNA3.1_spike_del19) using Lipofectamine 2000 (Thermo Scientific). The culture medium was replaced after 6h ...
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bioRxiv - Immunology 2022Quote: ... The trimeric spike SARS-CoV-2 and its variants were biotinylated (EZ-Link Sulfo-NHS-Biotin, ThermoFisher), desalted (Zeba Spike Desalting ...
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bioRxiv - Bioengineering 2022Quote: ... or SARS-CoV-1-d28 (Genbank AAP13567) or SARS-CoV-2 VOC spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and CCR5: rabbit anti-SARS-CoV-2 S1 protein (PA5-81795; ThermoFisher) at 1:2,500 ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 genome was amplified by using Ion AmpliSeq SARS-CoV-2 Research Panel (ThermoFisher Scientific, USA) that consists of two pools with amplicons ranging from 125 bp to 275 bp in length and covering >99% of the SARS-CoV-2 genome ...
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bioRxiv - Molecular Biology 2021Quote: 2 × 20 μg of each of the three different SARS-CoV-2 spike proteins were mixed with 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and up to 10 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000, Thermo Fisher Scientific PA5-112048), and an anti-p24 monoclonal antibody (1:1,000) ...
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bioRxiv - Microbiology 2021Quote: ... anti-SARS-CoV-2 (PA1-41098, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... SARS Cov-2 nucleocapsid (Thermofisher, 1: 1,000).
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bioRxiv - Pathology 2020Quote: ... and SARS Spike protein (Thermo Fisher, 1:250), and with Phalloïdin-647 (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 Spike ectodomain and the sRBD were transiently transfected in Freestyle ExpiCHO-S cells (Thermo Fisher). ExpiCHO cells were maintained and transfected according to the manufacturer using the “High Titer” protocol ...
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bioRxiv - Immunology 2021Quote: The codon optimized SARS-CoV-2 Wu01 spike (EPI_ISL_40671) was cloned into pCDNA™3.1/V5-HisTOPO vector (Invitrogen). SARS-2-S global strains (BavP1 EPI_ISL_406862 ...
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bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
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bioRxiv - Microbiology 2022Quote: ... biotinylated SARS-CoV-2 Spike trimer (Hexapro) was incubated with red streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... purified SARS-CoV-2 Spike and RBD were biotinylated using the EZ-Link NHS-Biotin (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... or N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher) as previously described [18] ...
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bioRxiv - Immunology 2020Quote: ... and N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher), i.e. ...
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bioRxiv - Microbiology 2019Quote: ... USA. Escherichia coli DH5α (hereafter called E. coli) was purchased from Invitrogen Inc. ...