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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Microbiology 2022Quote: ... Nb fused with Fc was purified using Protein G (cat.# 20399, Thermo Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... The M25 scFv-Fc was immobilized onto protein A beads (Thermo Fisher Scientific) and cross-linked by dimethyl pimelimidate dihydrochloride (DMP ...
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bioRxiv - Cancer Biology 2022Quote: ... VH-Fc proteins were expressed in the Expi293 expression system (Thermo Fisher Scientific) and purified with protein A resin (GenScript) ...
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bioRxiv - Physiology 2023Quote: ... Fc-FGF-21CT was purified using protein A sepharose 4B (Thermo Fisher Scientific) and dialyzed against PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits ...
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bioRxiv - Neuroscience 2021Quote: ... Secreted dimeric C-terminally Fc-tagged MDGA1 stably expressed in HEK293T cells was collected in serum-free Opti-MEM (Thermo Fisher Scientific, Inc.). Fc-tagged MDGA1 protein was run on an affinity column packed with Protein-G Plus Agarose fast flow resin (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
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bioRxiv - Cancer Biology 2020Quote: ... 10ng/ml recombinant human basic Fibroblast Growth Factor (bFGF) and 1x B27 supplement (Life Technologies). Cells were then counted and passed through a 25G needle to obtain a single cell suspension ...
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human TNF-α (PHC3011) and antibody against OAS3 (PA5-31090) was from Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Pathology 2022Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (Gibco) (S12+ media) ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Thermo Fisher Scientific AG), 1% penicillin and streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Immunology 2024Quote: ... and recombinant human interleukin 4 (rhIL-4) (300 U/mL; Thermo Fisher Scientific, Waltham, MA). On day 2 and day 5 post-monocyte isolation ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant human antibodies (rHumAbs) were desalted (Zeba™ Spin Desalting Columns, Thermo Fisher 89891) and concentrated using a Christ Rotational Vacuum Concentrator System ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and plated in the fibroblast medium onto truncated recombinant human vitronectin (VTN-N, Gibco, A14700)-coated plates ...
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bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
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bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Cell Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher Scientifc) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were captured by incubation with high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) for 3 hours at 4 degrees Celsius ...
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bioRxiv - Physiology 2020Quote: ... Biotinylated cell surface proteins were affinity-purified using NeutrAvidin-conjugated agarose beads (Thermo Fisher Scientific), and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were prepared and the biotinylated proteins were precipitated with streptavidin agarose resin (ThermoFisher Scientific) and detected by western blot.
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bioRxiv - Microbiology 2021Quote: ... University of Utah) and biotinylated proteins were detected with streptavidin Alexa-Fluor-488 (Invitrogen S32354). Primary antibodies were detected with goat polyclonal Alexa Fluor-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... Phages bound to biotinylated target protein were captured by streptavidin coupled Dynabeads (Thermo Fisher Scientific), washed and eluted in 100 nM triethylamine ...
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bioRxiv - Molecular Biology 2019Quote: ... biotinylated proteins were isolated by incubation with 200μl of Dynabeads (MyOne Streptavidin C1; Life Technologies) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Cell surface proteins were biotinylated using an EZ-Link Sulfo-NHS-Biotin reagent (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated proteins were enriched from the supernatant on High-Capacity Neutravidin Agarose resin (ThermoFisher Scientific) (0.5 mL of 50% resin slurry) ...
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bioRxiv - Neuroscience 2022Quote: ... (iv) Biotinylated proteins are affinity-purified with streptavidin agarose resin (Thermo Scientific, Cat no. 20349). The use of neutravidin agarose resin yielded the same results ...
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bioRxiv - Cancer Biology 2023Quote: ... and biotinylated proteins were extracted from the lysates using Pierce Streptavidin Magnetic Beads (ThermoFisher 88816) per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... the biotinylated LRRK2RCKW protein was immobilized to MyOne T1 streptavidin-coated beads (Thermo Fisher Scientific). The ribosome display selections were performed using a KingFisher Flex MTP96 well platform as described previously (Dreier and Plückthun ...
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bioRxiv - Immunology 2023Quote: Protein antigens were biotinylated using EZ-link Sulfo-NHS-Biotin No-Weigh kit (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... was biotinylated (Invitrogen) to a ratio of 1 biotin molecule per OVA as previously described (Brooks et al. ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then incubated using FITC-labelled goat-anti-human IgG-FC antibody (ThermoFisher Scientific) (200 ng/ul ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Microbiology 2021Quote: ... R-phycoerythrin (PE) conjugated goat anti-human IgG Fc secondary antibody (Invitrogen, Cat#12-4998-82) and PE conjugated mouse anti-PSGL-1 mAb (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... competition was detected by HRP-goat anti-human IgG Fc secondary antibody (A18817, Thermo Fisher Scientific). The kinetics of the antibody fragments were determined using a Biacore X100 (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc or DC-SIGN-Fc (Thermofisher) in binding buffer (BB ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the lysates was measured via BCA assay and biotinylated proteins were immunoprecipitated using Streptavidin magnetic beads (Thermo Fisher). 500μg magnetic beads were incubated with up to 70μg of lysate rotating overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was used for the enrichment of biotinylated proteins using100 μl Pierce™ streptavidin agarose beads (Cat. # 20353, ThermoFisher) overnight at 4 °C ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...