Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were eluted in 1.5x NuPAGE LDS Sample buffer (Invitrogen #NP0007) containing 20mM DDT (SRL #17315 ...
-
bioRxiv - Plant Biology 2023Quote: ... The biotinylated protein extract was incubated with 100 μL Streptavidin resin (Invitrogen) for 60 min at 25°C with mild shaking in the dark ...
-
bioRxiv - Developmental Biology 2023Quote: ... the biotinylated proteins were enriched by Dynabeads MyOne Streptavidin C1 (Invitrogen, #65001). The proteins bound to the beads were reduced with 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were purified using streptavidin magnetic beads (Thermo Fisher Scientific, 88816). Streptavidin magnetic beads were washed twice with RIPA containing HALT and quenching agents ...
-
bioRxiv - Molecular Biology 2024Quote: ... Biotinylated proteins were pulled down using streptavidin-magnetic beads (Dynabeads, Thermo Scientific) and eluted with 1% 2-mercaptoethanol ...
-
bioRxiv - Physiology 2023Quote: ... Biotinylated proteins were pulled-down with Pierce NeutrAvidin Agarose (ThermoFisher Scientific; 29200) at room temperature for 1 h mixed end-over-end ...
-
bioRxiv - Immunology 2023Quote: The DNA coding sequences of tagged recombinant GITRL and OX40L were produced by GeneArt Gene Synthesis service (ThermoFisher Scientific). The sequences of GITRL and OX40L constructs contain ...
-
bioRxiv - Immunology 2021Quote: All recombinant proteins were produced using Expi293F cells (Life Technologies). Spike proteins for enzyme-linked immunosorbent assay (ELISA ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was expressed using Expi293F™ Cells (ThermoFisher Scientific) following manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant proteins or commercially available RNase T1 (Thermo Fisher Scientific) were mixed with 0.5 pmol substrate RNAs in 10 µl RNase reaction buffer with EDTA (20 mM Tris-HCl pH7.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... Concentrations of recombinant proteins were measured by NanoDrop OneC (ThermoFisher).
-
bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were prepared by Expi293 Expression System (Thermo Fisher) according to the manufacturer’s instruction ...
-
bioRxiv - Genetics 2020Quote: ... 40 μL of recombinant protein G agarose beads (Thermo Fisher) were washed three times with 1 mL of ChIP dilution buffer by resuspending beads in the buffer ...
-
bioRxiv - Immunology 2021Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into phosphate buffered saline (PBS ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
-
bioRxiv - Physiology 2022Quote: Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
-
bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore) ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 µg (60 pmol) of recombinant Cas9 protein (A36498, Invitrogen) was incubated with 180 pmol of sgRNA in a 20 µL reaction for 20 minutes at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
-
bioRxiv - Immunology 2024Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher, Cat. #A14635) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher ...
-
bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
-
bioRxiv - Neuroscience 2021Quote: ... The presence of secreted Fc Fusion protein in the medium was confirmed by immunoblotting with goat anti-human IgG antibody (Invitrogen, A-21433, 1:500).
-
bioRxiv - Neuroscience 2021Quote: ... Cell surface proteins were biotinylated and isolated using Pierce Cell Surface Protein Isolation Kit (#89881, Thermo Fisher). All the steps were performed at 4°C unless otherwise mentioned ...
-
bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
-
bioRxiv - Immunology 2021Quote: ... was coated with 2μg/mL goat anti-human Fc antibody (Thermo Fisher Scientific) and incubated overnight at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... USA) and anti-human IgG Fc specific HRP conjugated secondary antibodies (ThermoFisher Scientific) were added ...
-
bioRxiv - Immunology 2022Quote: ... medium supplemented with 10 ng/ml human GM-CSF and 20% FCS (Gibco). For the purposes of proliferation assays ...
-
bioRxiv - Immunology 2023Quote: ... or HRP-conjugated rabbit anti-human IgG Fc antibodies (Invitrogen, 31423, 1:5,000) diluted in blocking buffer were added to the wells incubated with phages or recombinant proteins ...
-
bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
-
bioRxiv - Immunology 2023Quote: ... mammalian expression constructs carrying C-terminal 6X-His tagged gene of interest (HpARI1-3, or ST2 ectodomain) were transfected individually into Expi293F cells (Thermo Fisher) using the Expifectamine transfection kit (Thermo Fisher) ...
-
bioRxiv - Immunology 2020Quote: ... and 1µg/ml biotinylated recombinant hemagglutinin (rHA) (Whittle et al., 2014) conjugated to streptavidin APC (cat. no: S868, Invitrogen). To exclude dead cells ...
-
bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
-
bioRxiv - Immunology 2021Quote: ... FACS-sorted cells were suspended at 0.5×106 cells/mL in RPMI 1640 medium supplemented with 10% HI-FCS and 1% PenStrep (10378016; Gibco). CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well ...
-
bioRxiv - Cell Biology 2021Quote: ... GFP-tagged proteins were immuno-precipitated with the A11122 antibody (Thermo Fisher Scientific); Myc-tagged proteins were immuno-precipitated with the 9E10 antibody (Merck) ...
-
bioRxiv - Molecular Biology 2020Quote: ... GFP-tagged viral proteins were immunoprecipitated with anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
-
bioRxiv - Microbiology 2021Quote: ... Tagged proteins were visualized using the SuperSignal™ West Femto detection kit (ThermoFisher).
-
bioRxiv - Cell Biology 2022Quote: His8-tagged NR2B proteins were labelled with Alexa 647 NHS ester (Thermo Fisher) and PrLD-SAMWT/PrLD-SAMME/PrLD proteins labelled with Alexa 555 maleimide (Thermo Fisher) ...
-
bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected using SuperSignal West Femto: SuperSignal substrates (ThermoFisher Scientific) in a 1:1 ratio ...
-
bioRxiv - Plant Biology 2023Quote: ... The GST-tagged proteins were resuspended in phosphate-buffered saline (PBS) (Thermo Fisher) containing 1 mM dithiothreitol (DTT ...
-
bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with IgG-coupled Dynabeads (tosylactivated M280, Thermo Scientific), RNAPII with antibody against Rpb1 (8WG16 ...
-
bioRxiv - Biochemistry 2024Quote: ... AncΔ5 were tagged with Alexa Fluor 488 Microscale Protein Labeling Kit (Thermo Fisher), followed by the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... following which protein-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) following manufacturer’s instructions ...
-
Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
-
bioRxiv - Biochemistry 2021Quote: ... ACE2-Fc was purified over Protein A Agarose (Thermo Fisher; 15918014). Once the Protein A Agarose was equilibrated in PBS buffer ...
-
bioRxiv - Immunology 2023Quote: ... KIR-Fc were conjugated to protein-A Alexa 647 (Invitrogen, #P21462) overnight at 1:4 (molar ...
-
bioRxiv - Microbiology 2021Quote: ... or His tag was purified using Protein G (cat.# 20399, Thermo Scientific) and Ni-NTA (cat.# R901100 ...
-
bioRxiv - Systems Biology 2019Quote: ... Biotinylated probes were hybridized to GeneChip Human Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol ...