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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Full-length human LRRC4B was subcloned and inserted into the pcDNA6-V5-His vector (Invitrogen). FAM19A5 (NM_001252310.1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Human JAGGED1-Fc (Martin et al., 2023) was transfected into Expi293F cells (ThermoFisher, A14527) using FectroPro (Polyplus ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v) Heat-Inactivated Fetal Bovine Serum (HI-FCS; A3840001; Gibco)] ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: Recombinant protein concentration was quantified through BCA protein assay kit (Thermo Fisher Scientific, Buenos Aires, Argentina) on a BioRad 3350 Microplate Reader using BSA as standard ...
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bioRxiv - Immunology 2023Quote: ... The concentrations of the purified recombinant proteins were determined by the BCA protein assay kit (ThermoFisher) and separated by reducing SDS-PAGE and visualized by silver staining ...
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bioRxiv - Immunology 2022Quote: ... The concentrations of the purified recombinant proteins were determined by BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RBD-hFc proteins were captured by Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), washed by wash buffer (100 mM Tris/HCl ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) with the following modifications ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... mono-Fc was removed using Pierce Protein A agarose beads (Thermo Scientific). The flow-through containing PvDBP-RII was then mixed with DB1 or DB9 Fab (1.5:1 Fab to protein ratio ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Human protein arrays (ProtoArrays V5.0, Life Technologies) kept at -20°C were equilibrated at 4 °C for 15 min and ...
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bioRxiv - Immunology 2020Quote: The assay to measure total IgG was modified to measure IgG subclass responses at 1/100 including a two-step biotinylated anti-human IgG subclass (mouse anti-human IgG1: HP6069, IgG2: HP6002, IgG3: HP6050 IgG4: HP6023, Thermo fisher Scientific, UK) with a streptavidin-Phycoerythrin tertiary (Thermo fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Biotinylated surface proteins were recovered by incubating 200 μg of total protein with streptavidin-agarose beads (20347, Thermo Fisher) and eluting in Laemmli sample buffer ...
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bioRxiv - Immunology 2023Quote: ... Purified P.ch MSP1 protein and purified P.y MSP1 protein were biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-PE (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Basal Medium plus 50 ng/ml human recombinant VEGFA (PHC9391 Gibco, Frederick, MD, USA) or Basal Medium plus 100 ng/ml recombinant human NTN1 (6419-N1 ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Recombinant tau monomers (human 4R2N T40/ Evotec, Germany) were labeled with Cy3 (Life Technologies). To prepare tau oligomers ...
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Bioengineering 2024Quote: ... and human and mouse DKK3 (hDKK3: AA 1-350, mDKK3: AA 1-349) triple tagged with Fc-Myc-6xHis were transfected into Expi293FTM (Thermo Fisher Scientific) cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were then purified using high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) via incubation for three hours at 4°C after which beads were washed extensively in dilution buffer plus 0.5M NaCl ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated proteins were coupled to FITC-labeled 1um FluoSpheres NeutrAvidin-labeled Microspheres (Thermo Fisher) at 1 ug to 1 ul ratio of protein to beads for 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... RBD protein was first biotinylated with EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) and then selected with streptavidin-coated magnetic Dynabeads™ M-280 (ThermoFisher) ...