Labshake search
Citations for Thermo Fisher :
1 - 50 of 1154 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
-
bioRxiv - Genetics 2019Quote: pET22b-mZCWPW1-His construct was transformed into BL21 (DE3) E.Coli cells (Invitrogen). Bacterial cultures were grown to a density with O.D600∼0.7 ...
-
bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: His-tagged relaxins were expressed as secreted proteins in Expi293F cells containing a stably integrated tetracycline repressor (Expi293F tetR, Thermo Fisher Scientific) grown in Expi293 media (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2019Quote: ... Stbl3 E.coli (Invitrogen) was used for all pCMV AAV vector cloning.The in-frame stop codon in the BGH 3’ UTR was removed to allow for readthrough into the poly(A ...
-
bioRxiv - Bioengineering 2022Quote: ... anchored oligo-dT primers (Operon) and Superscript III (Invitrogen). Primers for specific target genes were designed using the software Oligo Explorer 1.2 (Genelink) ...
-
bioRxiv - Biochemistry 2020Quote: ... with a N-terminal 8x His tag was expressed from the pSJ2 vector (84) in BL21 E.coli and purified by Ni-NTA resin (Thermo Fisher). Full-length human Src kinase (WT or K298M ...
-
bioRxiv - Microbiology 2021Quote: ... E.coli strain DH5α (Invitrogen) was first transformed with the pSIM5 plasmid (pSC101 repAts ...
-
bioRxiv - Molecular Biology 2023Quote: ... E.coli DH10B (Thermo Scientific) or E.coli XL10-Gold (Agilent Technologies ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and stably expressing the tetracycline repressor protein) were purchased from Thermo Fisher Scientific (R78007) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 200ng PB-LacI-eGFP and 200ng pBroad3_hyPBase_IRES_tagRFPt40 with Lipofectamine3000 (Thermo Fisher Scientific, L3000008) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... The amplified operon was TOPO-ligated to pEXP5-CT vector (Invitrogen), and transformed to E ...
-
bioRxiv - Synthetic Biology 2023Quote: ... BL21-AI E.coli (Thermo Fisher) were transformed with mCherry-pBAD (gift from Michael Davidson & Nathan Shaner & Roger Tsien ...
-
bioRxiv - Biophysics 2023Quote: ... cells stably expressing the tetracycline repressor using an Expifectamine transfection kit (Thermo Fisher) following the manufacturer’s recommendations with the following modifications ...
-
bioRxiv - Biophysics 2019Quote: E.coli strain BL21 (DE3) (ThermoFisher Scientific) was used for CBMCipA production ...
-
bioRxiv - Microbiology 2023Quote: ... Lactose metabolism was assessed by growth on MacConkey agar (Thermo Scientific; cat. CM0007B).
-
Acute cerebellar knockdown of Sgce reproduces salient features of Myoclonus-dystonia (DYT11) in micebioRxiv - Neuroscience 2019Quote: ... and oligonucleotides were commercially generated (Eurofins MWG Operon Oligos Tool, Thermo Fisher Scientific). Each 10 µl reaction contained 300nM each of forward and reverse primer and 100 ng RNA ...
-
bioRxiv - Microbiology 2020Quote: ... An L5attP-zeoR-cydABDC-operon complementing plasmid was assembled by Gateway reaction (Invitrogen) and transformed into the hygR cydA mutant to generate the cydABDC-complementing strain ...
-
bioRxiv - Cell Biology 2019Quote: ... E.coli BL21 Star (DE3) cells (Life Technologies) transformed with pPR-IBA2-CLASP2 (1213-1515 ...
-
bioRxiv - Genomics 2019Quote: ... Plasmids were transformed into GT115 E.coli (Invitrogen) and DNA isolated using PureYield Plasmid Midiprep system (Promega) ...
-
bioRxiv - Biophysics 2020Quote: ... and expressed in E.coli BL21 (DE3) (Invitrogen). The wt-p53 (1-321 aa ...
-
bioRxiv - Genetics 2020Quote: ... ccdB resistant chemically competent E.coli (Invitrogen, A10460) were transformed with plasmids containing a Gateway cassette (ccdB ...
-
bioRxiv - Neuroscience 2019Quote: ... microglia were incubated with 50 µL of E.coli particle suspension (pHrodo™ Green E.coli BioParticles™, P35366, Invitrogen™) for 60 min ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 stably expressing the tetracycline (tet)-repressor (Flp-In T-REx HEK293, Invitrogen, Carlsbad, CA, USA) were cultured as above with the addition of 50 μg/mL zeocin and 5 μg/mL blasticidin (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... Inverter cassettes based on the SrpR and PhlF repressors were obtained as synthetic genes (GeneArt, ThermoFisher) and were likewise introduced into pCrepusculo ...
-
bioRxiv - Immunology 2021Quote: ... 100uL of 1mg/mLpHrodo Red E.coli BioParticles (Invitrogen) were added in PBS and cells placed at 4°C (negative control ...
-
bioRxiv - Genetics 2019Quote: ... and transformed into E.coli DH5α competent cells (Invitrogen) for plasmid purification and validation through Sanger sequencing.
-
bioRxiv - Genomics 2022Quote: ... 0.14 U E.coli DNA Ligase (Invitrogen, 18052-019), 0.56 U E.coli DNA Polymerase I (Invitrogen ...
-
ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... pHrodo® Red E.coli Bioparticles® (Life Technologies) were resuspended in 2 ml of Live Cell Imaging Solution (Cat ...
-
bioRxiv - Cell Biology 2023Quote: ... transformed into E.coli BL21(DE3) (Thermo Fisher Scientific) and purified as described before (Ganz et al. ...
-
bioRxiv - Microbiology 2022Quote: ... Mel Juso cells stably expressing the Tet Repressor from the pcDNA™ 6/TR vector (Life Technologies) were made following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: siRNA purchased from Eurofins MWG Operon (Table 1) were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) following manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... The operon gtrABC was amplified from the chromosome of SB300 using the Phusion Polymerase (ThermoFisher Scientific) and primers listed Table S2 ...
-
bioRxiv - Genetics 2021Quote: ... of One Shot TOP10 Chemically Competent E.Coli (ThermoFisher #C404003) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... Delivery of pHrodo Red E.coli BioParticles Conjugate (Molecular Probes) was performed with a similar procedure at - 2.5 mm A-P ...
-
bioRxiv - Genomics 2022Quote: ... 0.56 U E.coli DNA Polymerase I (Invitrogen, 18010-025), 0.03 U RNase H (Invitrogen ...
-
bioRxiv - Genomics 2020Quote: ... This was subsequently transformed into ccdB Survival E.coli (Invitrogen).
-
bioRxiv - Neuroscience 2021Quote: ... pHrodo™ Green E.coli Bioparticles (ThermoFisher, P35366; 3μg/ml), pHrodo™ Red LDL (ThermoFisher ...
-
bioRxiv - Synthetic Biology 2020Quote: Proteins were expressed from E.coli BL21-AI strains (Invitrogen) carrying appropriate plasmids for expression of E.coli Gnd or E.coli RpiA (ribose-5-phosphate isomerase ...
-
bioRxiv - Cell Biology 2023Quote: ... with Top10 E.coli competent cells (#K450001SC, ThermoFisher, Waltham, MA) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: Plasmids were amplified using E.Coli DH5α strain (Life Technologies). DNA was then purified using the NucleoBond® Xtra Plus Midi-Prep kit (Machery-Nagel®) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Ligated plasmids were transformed into DH5α E.coli (Life Technologies). The PX330-Alk-Eml4 plasmid was kindly provided by Andrea Ventura [26].
-
bioRxiv - Biochemistry 2022Quote: ... and LacI antibodies were affinity-purified from serum using the appropriate antigen coupled to AminoLink Coupling Resin (Thermo Scientific) according to manufacturer’s protocol.
-
Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
-
bioRxiv - Biochemistry 2021Quote: ... Heterostable cell lines expressing the tetracycline repressor and XBP1s were then selected using 10 μg/mL blasticidin (Gibco) and 50 μg/mg zeocin (Invitrogen) ...
-
bioRxiv - Molecular Biology 2020Quote: siRNA purchased from Eurofins MWG Operon (Supplementary Table 2) were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... the kdpFABC operon with the 8x histidine tag was cloned into the pBAD vector (Invitrogen, Carlsbad CA) and introduced into E ...
-
bioRxiv - Cell Biology 2019Quote: ... and transformation into DH5a competent E.coli cells (Thermo Fisher Scientific). Prior to injection plasmids were test digested and sequenced (Core Genomic Facility ...
-
bioRxiv - Biophysics 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids were propagated in Stbl3 chemically competent E.coli (ThermoFisher C737303). Plasmid sequences were verified by Sanger sequencing (GENEWIZ Inc).