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Citations for Thermo Fisher :
251 - 300 of 1154 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The plasmid pBAD/Myc-His A (pBAD) (Invitrogen) and pET28a (+ ...
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bioRxiv - Immunology 2024Quote: Dynabeads His-Tag Isolation and Pulldown beads (ThermoFisher) were washed once with Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2024Quote: C2C12 differentiation medium: DMEM Hi Glucose medium (Gibco) supplemented with 2% Horse Serum (Gibco ...
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bioRxiv - Biochemistry 2024Quote: Basal Conditioned medium: DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin – Streptomycin – Glutamine (Gibco ...
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bioRxiv - Biochemistry 2024Quote: DMEM Starvation medium: DMEM Hi Glucose medium (Gibco) supplemented with 0.2%heat-inactivated fetal bovine serum (US origin ...
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bioRxiv - Biochemistry 2024Quote: DMEM complete medium: DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin – Streptomycin – Glutamine (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... anti-6*his tag (Thermo Fisher Scientific,11533923), anti-myc tag (Abcam ...
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bioRxiv - Genetics 2021Quote: These PCR products were both visualized on an agarose gel as well as TA-cloned using ‘TA Cloning Kit with pCR2.1 vector and OneShot TOP10 Chemically Competent E.coli’ (ThermoFisher) according to kit instructions ...
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bioRxiv - Cell Biology 2021Quote: ... for 5min and then with hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Cell Biology 2021Quote: ... the tissues were mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Cell Biology 2020Quote: ... for 5 min and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Microbiology 2022Quote: ... amplicons of each target gene were cloned into competent E.coli cells using the TOPO Cloning Reaction Kit (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... Hybridization mix contained hybridization buffer (10% Dextran sulfate Sigma D8906, 30% Formamide, 1□mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Developmental Biology 2021Quote: ... and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 30% Formamide, 1 mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Immunology 2020Quote: ... 20⧅l of opsonised Escherichia coli (E.coli) was added to the tube (pHrodo Red E. coli BioParticles; ThermoFisher). The bacteria was initially prepared by mixing the E.coli strain with new-born calf sera followed by a 30-minute incubation at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated in hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with HBSS and incubated for 2 hours with 0.1 mg/ml of pHrodo E.coli bioparticles (Invitrogen) in HBSS ...
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Microbiology 2019Quote: ... His-FruB or His-HPr in the cell extract was purified using HisPurTM-Ni-NTA Superflow Agarose (from Thermo Scientific, Cat # 25214) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Genomics 2019Quote: ... the plasmids were multiplied into E.coli cells and then eluted with the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen). To ensure the presence of the inserts ...
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bioRxiv - Neuroscience 2021Quote: ... Constructs were validated by sequencing prior to site-specific recombination with the baculovirus genome provided by its host E.coli strain DH10Bac (Bac-to-Bac-System, Invitrogen). Positive recombinant clones were identified by blue-white screening and further confirmed by PCR ...
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bioRxiv - Biophysics 2021Quote: ... the protein was expressed as a fusion construct with an N-terminal His6 tag followed by tobacco etch virus (TEV) protease cleavage site in E.coli BL21 DE3 Star cells (Invitrogen). The fusion construct was purified using immobilized-nickel affinity chromatography ...
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bioRxiv - Microbiology 2021Quote: ... The DNA fragments containing NP and RBD were codon-optimized for E.coli expression and synthesized by GeneArt (Thermo Fisher). The plasmids containing wild-type (WT ...
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bioRxiv - Genetics 2019Quote: ... Recombinant entry clones were subjected to LR Clonase II reaction with the pJawohl-ACT2 destination vector (28) and introduced into E.coli DH5α (Invitrogen) cells by heat-shock transformation ...
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bioRxiv - Immunology 2019Quote: For activation of MAIT cells by bacteria-derived ligands, THP-1 cells were loaded with PFA-fixed (2%, 20min) E.coli (DH5α, Invitrogen) at a 25 bacteria per cell (BpC ...
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bioRxiv - Microbiology 2021Quote: ... the circular virus DNA was extracted from infected cells using the Hirt method (Hirt, 1967) and electroporated into E.coli ElectroMAX DH10B Cells (Invitrogen). Transformants were selected on LB agar plates with 12.5 µg/ml chloramphenicol and colonies were picked for further characterization.
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bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
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bioRxiv - Developmental Biology 2021Quote: ... and inserted into pMT/V5-His A (Thermo Scientific) using XhoI and SpeI target sites ...
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bioRxiv - Cell Biology 2022Quote: pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells ...
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bioRxiv - Molecular Biology 2019Quote: ... and Ion PGM Hi-Q Sequencing Kit (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-Penta-His-tag (#P-21315, ThermoFisher Scientific), 1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
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bioRxiv - Microbiology 2021Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or SARS-CoV-2 N-His (Sino Biological # 40588-V08B ...
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bioRxiv - Microbiology 2022Quote: ... or the mouse-anti-His (HIS.H8) primary antibody (Invitrogen) (1:1,000 ...
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bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2022Quote: ... Pre-washed 25 µl His-Tag Dynabeads (ThermoFisher Scientific) were added ...
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bioRxiv - Neuroscience 2019Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies against the His-tag (1:2000, Invitrogen) were followed by secondary HRP conjugated antibody (1:2000 ...