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Citations for Thermo Fisher :
451 - 500 of 1154 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... which is RPMI-1640 media containing 10% heat-inactivated (HI)-FBS (Gibco) and 1x penicillin-streptomycin-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... were run with 1X Hi-Density TBE Sample Buffer (Thermo Fisher # LC6678) and 1X TBE Running Buffer ...
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bioRxiv - Immunology 2022Quote: ... Avi/His-tagged proteins were purified from transiently-transfected Expi293F cells (Gibco) by nickel affinity chromatography and SEC (Barnes et al. ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher), and rabbit polyclonal anti-mCherry (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: TOPOVIBL452-579 was cloned as a His-tag fusion into pProEXHTb (Invitrogen). REC114-MEI4 complex and TOPOVIBL452-579 were individually expressed in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10-15 mL of His-Pur Ni-NTA resin (Thermo Fisher Scientific) was used per L of cell culture ...
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bioRxiv - Cell Biology 2023Quote: ... His-VASP was bound to HisPur™ Ni-NTA Resin (ThermoFisher #88221), washed twice with 5 CV of wash buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 6x His-tag monoclonal primary antibody (MA1-135; ThermoFisher) overnight at a dilution of 1:750 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher) and mouse monoclonal anti-FLAG (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with 2 µg/ml anti-His (Invitrogen #MA1-21315) over night and blocked for 1hour as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % heat inactivated Fetal Bovine Serum (HI FBS) (10500064; Gibco) and 1x antibiotic-antimycotic drug (100 units/mL of penicillin ...
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bioRxiv - Immunology 2023Quote: His-tagged proteins were purified with HisPurTM Ni-NTA resin (Thermo Fisher). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, USA) and 100 U mL-1 penicillin–streptomycin (Gibco ...
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bioRxiv - Biophysics 2024Quote: ... with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X) (Invitrogen). The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (V/V) fetal bovine serum (HI FBS heat inactivated, Gibco) and GlutaMAX-I (Gibco)39 ...
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bioRxiv - Microbiology 2019Quote: ... 1x Pen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific), and pelleted at 350 xg for 5 min ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat ...
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bioRxiv - Immunology 2021Quote: ... HI sera were tested using PrioCHECK PRRSV Antibody ELISA Kit (Thermo Fisher Scientific) for detection of PRRSV N protein-specific antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and anti-His tag (Alexa Fluor 555 conjugated, Thermo Fisher MA1-21315-A555) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... It was first amplified and inserted into pMT/V5-His B (ThermoFisher Scientific), using primers that introduced EcoRI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2020Quote: ... Gβ1 and His-Gγ2 (C68S) were synthesised and cloned into pFastBac Dual (Invitrogen). For mammalian expression ...
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bioRxiv - Biochemistry 2020Quote: N-terminally His-tagged P-Rex2 was synthesised and cloned into pFastBac1 (Invitrogen). His-tagged PTEN ...
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bioRxiv - Molecular Biology 2019Quote: ... Results were detected using Western Blotting using anti-His primary antibody (ThermoFisher, His.H8).
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bioRxiv - Biophysics 2019Quote: ... dye free liposome was prepared with TexasRed conjugated anti-His tag Antibody (ThermoFisher) by mixing lipids with antibody containing buffer ...
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bioRxiv - Microbiology 2021Quote: ... and subcloned into expression vector pBAD/myc-His A (Invitrogen, Carlsbad, CA, USA) that produces a fusion six-histidine tag at the end ...
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bioRxiv - Bioengineering 2021Quote: ... Blocking buffer was refreshed and mouse anti-His antibody (Thermofisher, Cat#MA1-21315) was added at a dilution factor of 1:2000 in blocking buffer ...
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bioRxiv - Biophysics 2021Quote: ... We used a rabbit anti-6X-His antibody (Invitrogen™, Waltham, MA, USA) to detect histidine-tagged proteins or mouse 1A9 antibody (GeneTex ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing was performed with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific) using Ion Proton instrument ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10 % V/V heat inactivated Fetal Calf Serum (Hi FCS, Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS- HI; Gibco) at 37 °C in a humidified 5% CO2 incubator ...
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bioRxiv - Cell Biology 2021Quote: The RNAP2 Ser2ph-mintbody sequence was inserted into pTrc-His (Thermo Fisher Scientific) to harbor the His-tag at the N-terminus ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using the Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher). Sequence reads were mapped to the hg19_AmpliSeq_Transcriptome_ERCC_v1.fasta reference ...
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bioRxiv - Microbiology 2020Quote: ... and His-PbChiB4 were purified with Ni-NTA agarose (Thermo Fisher Scientific, CAD) through native conditions following the manufacturer‘s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-His (C-term)-HRP (#46-0707, 1:2000 for dot blot, Invitrogen); Aβ (6E10 ...
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bioRxiv - Microbiology 2021Quote: ... PCR-mediated mutagenesis was performed using Phusion Hi Fidelity polymerase (Thermo Fisher Scientific) with the following thermocycler settings ...
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A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccinesbioRxiv - Cancer Biology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS, GIBCO, Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (10,000 U/mL ...
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A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccinesbioRxiv - Cancer Biology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS, GIBCO, Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (10,000 U/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Samples (2 µL) were suspended in 10 µL Hi-Di formamide (Thermo Fisher) and 0.5 µL MapMarker ROX 1000 (Eurogentec ...
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bioRxiv - Genetics 2020Quote: ... reactions were diluted with Novex Hi-Density TBE Sample Buffer (Thermo Fisher LC6678), and run on precast TBE gels ...
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bioRxiv - Molecular Biology 2022Quote: ... PfP2 sample was purified after binding with Hi-Bind Ni-NTA beads (Invitrogen) for 4h followed by elution of the sample with 20mM Tris-HCl (pH 7.4) ...
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bioRxiv - Developmental Biology 2020Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... The His-tagged proteins were purified on Ni-NTA resin (Thermo Scientific #88222) and washed into a buffer containing 50 mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Microbiology 2021Quote: ... The products were cloned into the pcDNA3.1D/V5-His’s Topo expression vector (Invitrogen) and NL4.3 ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His Tag Antibody (clone HIS.H8) (1:1000, # MA1-21315) was from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and into pVL847 (His-MBP-tag overexpression vector) by Gateway cloning method (Invitrogen). ΔnahA mutant of Bacillus subtilis was constructed by CRISPR/Cas9 editing method (Ref) ...
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bioRxiv - Molecular Biology 2021Quote: Western blot analysis was performed using an anti-His antibody (Invitrogen, LOT 1902132) to confirm the identity of splicing and cleavage products ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Thermo Fisher Scientific) and 1% Pen-Strep (Thermo Fisher Scientific ...