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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cells were gently washed twice in ice-cold 1X PBS and lysed using ice-cold cell lysis buffer (50 mM Tris-HCl, 2% w/v SDS, 10% glycerol) containing 1X HALT (dual phosphatase and protease inhibitor, Thermo Fisher). Lysates were sonicated at 20% amplitude for 20 seconds ...
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bioRxiv - Biophysics 2020Quote: ... 1 μL of 20 mM latrunculin A or 1 μL of DMSO was mixed with 100 μL of YE5S medium containing 2% (w/v) agarose UltraPure agarose (Invitrogen Corporation) kept in a water bath at ~70 °C ...
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bioRxiv - Microbiology 2022Quote: ... a second agarose overlay containing DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) and 200 ng/mL rifampicin was added 16 – 24 h after infection ...
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bioRxiv - Microbiology 2021Quote: ... 5µl were subjected to quantitative PCR using primer/probe sets for human Gapdh (Applied Biosystems), and EBOV trVLP 5’-trailer region ...
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bioRxiv - Pathology 2021Quote: ... Human Spred2 probe set was purchased from Thermo Fisher (Affymetrix, Catalog No. VA1-17417-01). Spred2 mRNA expression was stained in red-dot ...
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bioRxiv - Cancer Biology 2024Quote: ... Maximum release was set up with 2% Triton X-100 (Acros Organics, Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... or 2% (w/v) galactose (ACROS Organics™) was used for the cultivations ...
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bioRxiv - Immunology 2023Quote: ... 2% w/v BSA in PBS (Fisher Scientific) was used to block the wells for 2h at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... and 2 % (w/v) NHS (Thermo Fisher, 22500). Then ...
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bioRxiv - Cell Biology 2021Quote: MS/MS spectra were searched against a Swiss-Prot human database containing 20,341 reviewed protein entries using Mascot algorithm (Matrix Science) via Proteome Discoverer 2.2 (PD, Thermo Fisher Scientific) and were processed as previously described (10) ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Germany and cultured in TBGG medium containing 0.2% v/v Penicillin/Streptomycin (Life Technologies, Carlsbad, CA, USA) and 0.05% w/v Hemin (MP Biomedicals ...
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bioRxiv - Cell Biology 2019Quote: ... minced and digested with trypsin (0.05% (v/v) solution containing 0.53 mM EDTA) (Gibco, Carlsbad, CA, USA) and 1.8 mg/ml DNase I (Roche ...
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bioRxiv - Immunology 2021Quote: ... University of Birmingham) was passaged in 10% FBS (v/v) RPMI containing 1% penicillin/streptomycin (Life Technologies). On day of experiment ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were desalted with 100% water containing 0.1% formic acid v/v (LC-MS grade, Fisher Scientific) for 3 minutes at a flow rate of 100 μL/min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were cultured in high glucose (4.5 g/L) Dulbecco’s Modified Eagle’s Medium containing 10% (v/v) fetal bovine serum (Gibco). The GPR37L1 ...
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bioRxiv - Cell Biology 2023Quote: ... containing 10% (v/v) heat-inactivated FBS and 100 U/mL penicillin and streptomycin (Life Technologies Australia). All cells were kept at 37°C and 5% CO2.
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bioRxiv - Bioengineering 2021Quote: ... STEMdiff APEL2 medium (1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific) and 1% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... supplemented with 1% (v/v) PFHM-II Protein-Free Hybridoma Medium (Thermo Fisher Scientific, # 11370882), hereafter referred to as APEL™2 ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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bioRxiv - Cancer Biology 2022Quote: ... the ST1 cells were transfected with NICD pCMV Neo/intracellular domain of human Notch1 (NIC-1) plasmid using Lipofectamine 200 (Invitrogen), and then were harvested 48 hours after transfection for western blot and in vitro angiogenesis assay.
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bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human or mouse ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously44 ...
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bioRxiv - Biophysics 2020Quote: The 2019-nCoV receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen). The 2019-nCoV RBD (residues Arg319-Phe541 ...
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bioRxiv - Molecular Biology 2020Quote: The catalytic domain of the human ST6Gal I (residue range 132-406) was expressed in a BL21(DE3) Escherichia coli strain (Invitrogen), using a polycistronic Ptac vector containing the CyDisCo system (Gąciarz et al ...
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bioRxiv - Molecular Biology 2023Quote: Human retinal epithelial cells (hTERT-RPE1, ATCC) were grown in 10% (v/v) FBS (Atlas) / DMEM (Thermo Scientific). Media was supplemented with 1 mM L-glutamine (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... IL-2 was measured by human IL-2 ELISA (Invitrogen) using the manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: Human protein arrays (ProtoArrays V5.0, Life Technologies) kept at -20°C were equilibrated at 4 °C for 15 min and ...
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bioRxiv - Bioengineering 2020Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen), 0.178 mM Adenine (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... G-CSF Recombinant Human Protein (Thermo fisher), IP injection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2022Quote: ... lysate-containing supernatants were collected and protein quantities were determined via BCA (Pierce™ BCA Protein Assay Kit, Thermo Scientific). Proteins were denatured by addition of 2X Laemmeli buffer/10% β-mercaptoethanol (Bio-Rad/Sigma ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Immunology 2022Quote: ... The gB Domain I and Domain I+II were purified using Nickel-NTA resin (Thermo Fisher Scientific), and the gB Domain II was purified with lectin resin (VWR.)
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bioRxiv - Immunology 2020Quote: ... Cecal contents were dissolved in fecal immunoglobulin extraction buffer (1X PBS, 0.5%Tween, 0.05% Sodium Azide) containing 1X protease inhibitor cocktail (Cat#78429, Thermo Fisher Scientific, Rockford, USA) and centrifuged at 1500 rcf for 20 minutes at 4°C to isolate the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Immunology 2020Quote: ... Murine IFNγ and IL-2 cytokines were measured in supernatants according to the Murine IFNγ and IL-2 ELISA Ready-SET-GO!® ELISA kit (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated for 2–3 min at RT in trypsin (0.05% v/v; Gibco 25300-054), and tapped to release ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM magnesium chloride and 1% v/v NP-40) with protease inhibitor cocktail (ThermoFisher). Glycoprotein denaturation of the cell lysate was performed with 1x glycodenaturing buffer (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... the media was changed to Neurobasal media with 2% (v/v) B-27 Supplement (Gibco) instead of FBS.
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bioRxiv - Cell Biology 2020Quote: ... 2 mM glutamax and 1% v/v 100x insulin transferrin selenium (ITS) (both from Invitrogen). The cells were incubated in a humidified incubator with 5% CO2 at 37°C and media renewed every 3 days for 21 days.
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bioRxiv - Plant Biology 2023Quote: ... 0.03% (w/v) L-glutamine (Alpha Caesar #A14201) 2% (w/v) sucrose (Fisher Scientific #10634932), and 100 μM acetosyringone ...
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bioRxiv - Biophysics 2023Quote: ... with 2% v/v 200-nm-diameter fluorescent carboxylate-modified beads (Fluospheres, Thermo Fisher Scientific), 0.05% v/v ammonium persulfate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... containing 2% B27 supplement (ThermoFisher A3582801), and 500 μM L-Glutamine (ThermoFisher 25030024)] ...
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bioRxiv - Neuroscience 2020Quote: ... containing 2% B-27 supplement (Gibco), 1% GlutaMAX (Gibco) ...