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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2023Quote: ... the lysates were centrifuged for 15 min at 16,000 g and the supernatant containing the proteins were subjected for protein determination using a commercial Bicinchoninic acid assay (BCA) kit (Thermo Fisher, Karlsruhe, Germany). 50 g of proteins were heat desaturated in SDS-sample buffer (supp ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Biochemistry 2019Quote: For mammalian expression a DNA polynucleotide encoding the transmembrane domain (residues 1-295) of GtACR1 optimized for human codon usage was cloned into the pcDNA3.1 vector (Life Technologies, Grand Island, NY) in frame with an EYFP tag ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Biochemistry 2020Quote: The cDNA encoding a fusion protein consisting of GST followed by a 3C cleavage site and the human SMAD2 MH2 domain (residues 241–465) was inserted into the MCSI of the pFastBac™ Dual vector (Thermo Fisher Scientific) in the Sal1 and Spe1 restriction sites ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants containing fly and cell lysates were quantified with Pierce™ BCA Protein Assay Kit (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... NGS was performed using primer sets that tiled the entire SARS-CoV-2 genome (Ion AmpliSeq SARS-CoV-2 Research Panel, ThermoFisher), with library preparation and sequencing performed on Ion Chef and S5 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% (v/v) triton X-100) and measured through BCA kit (Thermofisher Scientific®). Proteins (10–30 μg ...
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bioRxiv - Immunology 2022Quote: The Invitrogen ProtoArray® Human Protein Arrays (ThermoFisher Scientific) are high-density microarrays that contain more than 9,000 unique human proteins individually purified and arrayed onto a nitrocellulose-coated slide ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Beads were washed four times with PBS containing protease inhibitors and protein were labeled with 2 mM Alexa 488 C5 maleimide or Alexa 546 C5 maleimide (Invitrogen) overnight at 4 °C in the dark ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... was premised on a commercial human RNaseP primer/probe set (443328, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2022Quote: ... was premised on a commercial human RNaseP primer/probe set (443328, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2020Quote: ... Positive proliferation control wells were set up by plating 1 Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) for every 200 T-cells ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The kinesin-1 motor domain was PCR amplified from cDNA using gene-specific primers and cloned into the pCR-XL-2-TOPO vector (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) and 1% (v/v) penicillin/streptomycin (P/S) (all from Thermo Fisher Scientific, Waltham, MA, USA). Then ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant poly-histidine tagged ectodomains of the SARS-CoV-2 S proteins (614D and 614G) were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using HisTrap FF column (GE Life Sciences)18 ...
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bioRxiv - Microbiology 2021Quote: ... containing .07 mM Ca2+ and human keratinocyte growth serum (HKGS; ThermoFisher). The next day ...
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bioRxiv - Microbiology 2023Quote: ... containing 1:400 of Human BD Fc Block (ThermoFisher Scientific, BDB564219) per condition ...
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bioRxiv - Cell Biology 2019Quote: PC12 cells were grown at 37°C and 5% CO2 in RPMI containing and 10% (v/v) horse serum (ThermoFisher 26050088), 5% (v/v ...
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bioRxiv - Cell Biology 2020Quote: The remaining cell suspension was centrifuged at 300 × g for 5 min at RT and resuspended in 500 μL PBS containing 0.5% (v/v) Triton X-100 (Thermo Fisher Scientific) to facilitate cell lysis ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 2 mL of cold lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% Triton X100 (v/v) containing protease inhibitor (ThermoFisher; cat# 78429). Suspensions (2 mL ...
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bioRxiv - Immunology 2021Quote: ... or [4A] Ac-ASQARPSQR or [4Y] Ac-ASQYRPSQR (custom products from GL Biochem Shanghai) in 10% FBS (v/v) RPMI containing 1% penicillin/streptomycin (Life Technologies) at 37°C and 5% CO2 for the indicated time points.
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bioRxiv - Bioengineering 2022Quote: ... Nerve sections were transferred to mammalian physiological saline solution containing the live stain fluoromyelin (1:250 v/v in mammalian physiological saline; Invitrogen, F34651) and incubated for 1 hr at room temperature to stain the myelin surrounding axons ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then spun down and resuspended in an extracellular matrix (ECM)-rich mixture containing 40% DMEM (%v/v, Gibco, 11995073), 40% fibrinogen (10 mg/mL stock concentration in a 0.9% NaCl water solution ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 37 °C in 2X SSC containing 10% v/v Formamide and DAPI (Thermo Fisher Scientific D1306). Finally ...
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bioRxiv - Bioengineering 2020Quote: ... C2C12s were cultured in media composed of high glucose DMEM supplemented with 2 v/v% horse serum (Gibco) and 1 v/v% penicillin streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... The digestant was diluted into 2% v/v high purity HNO3 (Optima; Fisher Scientific Company; Nepean, ON, CA) in acid-washed 15-mL polypropylene tubes ...
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bioRxiv - Microbiology 2022Quote: ... Viral stocks were prepared by propagation in Vero E6 cells in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% (v/v) fetal bovine serum (FBS, Invitrogen). Titres of virus stocks were determined by plaque assay.
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bioRxiv - Bioengineering 2022Quote: ... in IMDM/F12 medium supplemented with 1% (v/v) N-2 (Thermo Fisher Scientific, catalogue no. 17502-048), 1% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... culture medium was replaced by differentiation medium consisting in DMEM and 2% v/v horse serum (Gibco™) and samples were cultured for 6 more days without medium replacement ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Molecular Biology 2020Quote: ... and digestion with a solution containing type II collagenase (0.2% w/v; Gibco) and trypsin (0.25% w/v ...
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bioRxiv - Immunology 2019Quote: ... pH 8.0) containing protease inhibitors (Protease V cocktail, Calbiochem or Halt-protease, Invitrogen), 1mM sodium orthovanadate (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 0.1% bovine serum albumin (BSA) fraction V (Thermo Fisher Scientific, 15260-037) and 30 mM HEPES (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Following gel electrophoresis [1% (w/v) agarose in TAE containing SYBR-safe (ThermoFisher)] the DNA products were visualized using an Image Station 4000M PRO gel system running Carestream software.
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bioRxiv - Neuroscience 2021Quote: A solution containing 10% w/v Texas Red dextran (TRD, 3,000 MW, Invitrogen) in water was pressure injected in the spinal cord (between body segment 7 to 14 ...
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bioRxiv - Microbiology 2023Quote: ... was added to BHIS containing 0.1% (w/v) deoxycholic acid (DCA) (Fisher Scientific). Control samples contained either no isotopically labeled amines ...
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bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... appended with relevant exogenous protein sequences and common protein contaminants using the MS Amanda v.2.0 algorithm in Proteome Discoverer v.2.3 (Thermo Scientific). As described previously(Walker et al. ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10-100 μg total protein was then separated with a 4-12% (v/v) gradient Bis-Tris gel (ThermoFisher) run in MES buffer system ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein and DNA were crosslinked by incubating the discs in 1.8% (v/v) formaldehyde (Thermo Fisher Scientific, Cat# 28906) in 1x PBS for 15 min at RT and quenched with 225 mM Glycine for 5 min at RT (Oh et al. ...
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bioRxiv - Immunology 2023Quote: ... Proteins were identified by database searching of the fragment spectra against the SwissProt (EBI) protein database using Mascot (v 2.6, Matrix Science, London, UK) and Proteome Discoverer (v 2.2, Thermo Scientific). Typical search settings were ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were identified by database searching of the fragment spectra against the SwissProt (EBI) protein database using Mascot (v 2.6, Matrix Science, London, UK) and Proteome Discoverer (v 2.2, Thermo Scientific). Peptides were subject to 1% FDR using reverse-database searching.