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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Immunology 2021Quote: Human foreskin fibroblasts (HFFs) monolayers were grown in DMEM (4.5 g/L D-glucose) (Life Technologies) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/ml human basic FGF (R&D) on either CELLstart Substrate (A1014201, Gibco) or Vitronectin (A31804 ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Cell Biology 2023Quote: ... We observed that TAT-WT and TAT-R5K eGFP conjugates (but not eGFP alone) were contaminated with nucleic acids (A260nm signal on the Nanodrop (ThermoFisher Scientific) from the expression while eGFP alone was not ...
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bioRxiv - Cell Biology 2023Quote: ... co- transfected with lentiviral packaging plasmids (pCEP4-tat, pHEF-VSVG and pNHP) and lentiviral expression plasmids using Lipofectamine 2000 (Invitrogen, 11668019) as previously described50 with the following modifications ...
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bioRxiv - Genetics 2019Quote: ... and 5 µl of protein A: protein G 1:1 Dynabeads (Thermofisher). Antibody-bond chromatin was washed four times in low salt buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... A 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was prepared and 40 μL of the mixture was added to each well ...
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bioRxiv - Plant Biology 2019Quote: The DNA coding sequences for the 20 fluorescent protein (FP) genes listed in Table 1 were mobilized into Invitrogen pENTR /D-TOPO cloning vectors (Invitrogen, Carlsbad, CA). Following colony PCR and validation by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated on ice for 15 min and protein expression was initiated with the addition of 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Fisher Scientific; BP1755). Cells were then incubated for 16–18 hr at 18°C for optimal protein production.
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bioRxiv - Genomics 2020Quote: ... and subsequently subjected into nested polymerase chain reactions (PCR) according to manufacturer’s instructions prescribed in HIV-1 genotyping kit: Amplification module (Applied Biosystems, Life Technologies, Warrington, UK). The amplified cDNA was purified using ExoSAP-IT™ PCR product clean-up reagent (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Fragments hybridizing to biotin-labelled HIV-1 DNA were pulled down by incubating the hybridization reaction with streptavidin-conjugated magnetic beads (ThermoFisher Scientific, Cat#88816) at room temperature for 30 min ...
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bioRxiv - Immunology 2019Quote: ... The selected combinations of hrproteins or peptide demonstrated the highest reduction in HIV-1 p24 release without inducing tissue toxicity as indicated with a LDH assay (Pierce LDH Cytotoxicity Assay, Thermo Scientific, Rockford, IL).
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bioRxiv - Pathology 2023Quote: Total DNA was then extracted from HIV-1 infected cells at 6 h post-infection using the PureLink™ Genomic DNA Mini Kit (Thermo Fisher Scientific). Quantitative PCR for R-U5 and LTR-Gag transcripts was performed using the following primers:
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1% human keratinocyte growth supplement (HKGS, Gibco) and 1% pen-strep ...
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bioRxiv - Immunology 2022Quote: ... Sheep anti-human FH (1:200×, ThermoFisher) and then goat anti-sheep HRP (1:2000× ...
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bioRxiv - Cell Biology 2020Quote: ... Human Cot-1 (DNA) (Thermo Fisher Scientific) (10 μg per 2 μg of nick translated BAC vector ...
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bioRxiv - Neuroscience 2023Quote: ... anti-human IgG (Invitrogen, A21445, 1:400).
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bioRxiv - Microbiology 2023Quote: ... 1:2500 goat anti-human Ab (Invitrogen), 1:5000 goat anti-rabbit Ab (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... or human (1:2000; Thermo Fisher Scientific). DAPI was used to stain DNA (500 ng/mL ...
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bioRxiv - Microbiology 2024Quote: ... 1:1000 anti-human-AF594 (Molecular Probes) and 1:2000 L/D eFluor506 (Thermo Fischer Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... 115 mM D-Sucrose (Fisher Scientific, BP220-1), 5 mM Trehalose (Acros Organics ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-HuC/D (Invitrogen, A21271, 1:500), mouse anti-Gfap (ZIRC ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-HuC/D (Invitrogen, A21271, 1:1000), mouse anti-Hb9 (Developmental Studies Hybridoma Bank ...
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bioRxiv - Neuroscience 2022Quote: ... anti-HuC/D (1:100, Cat.# A21271; Invitrogen), anti-MITF (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... Isopropyl β-D-1- thiogalactopyranoside (IPTG; Thermo Scientific) was used for induction of gene expression and X-gal (X-Gal 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside ...
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bioRxiv - Plant Biology 2024Quote: ... Isopropyl β-d-1-thiogalactopyranoside (IPTG, ThermoFisher Scientific) was added to a final concentration of 1 mM to induce protein expression ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-HuC/D (1:500; Invitrogen). Secondary antibodies included ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-HuC/D (Invitrogen, A21271, 1:500). Secondary antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... DAPI (Invitrogen Molecular Probes D-21490, 1:5000). They were then mounted onto glass slides and coverslipped ...
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bioRxiv - Neuroscience 2023Quote: ... DAPI (Invitrogen Molecular Probes D-21490, 1:5000). They were then mounted onto glass slides and coverslipped ...
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bioRxiv - Neuroscience 2024Quote: ... calbindin D (ThermoFisher cat# MA5-50510, 1:1000), and glutamic acid decarboxylase (GAD ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Catalog Numbers 4444432). Briefly ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... Extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen) with oligo(dT ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Taqman Fast Virus 1-step Master Mix (Thermo Fisher Scientific, 4444434) in StepOne Plus real-time PCR thermocycler (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... vRNA was then reverse transcribed using the TaqMan Fast Virus 1-Step qRT-PCR kit (Invitrogen) and quantified on a LightCycler 480 (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Vero cells were infected with passage 9 virus and overlaid with 1% low melting agarose (Invitrogen) in MEM ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was conducted using TaqMan Fast Virus 1-Step Master Mix from Applied Biosystems in 384-well plates ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μl of RNAse-Out and 1 μl of the Moloney Murine Leukemia Virus reverse transcriptase enzyme (MML-V, Invitrogen). Quantitative PCR was carried out in a total of 15μl consisting of 3μl cDNA ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Biochemistry 2019Quote: ... LRRK2 (G2019S) Recombinant Human Protein (residues 970-2527 #10499963) was purchased from Invitrogen and all additional kinases used in the in vitro screen were from MRC PPU Reagents and Services – including TAK1 (1-303) ...
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bioRxiv - Neuroscience 2019Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: Drosophila Hsp40 and Hsp70 proteins were cloned by Topo-D-Entr cloning kit (Invitrogen) into Topo-D-Entr vector ...