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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... We used TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher Scientific) to set up 10 μl (half volume ...
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bioRxiv - Microbiology 2020Quote: ... containing 7,5 μl 4xTaqMan Fast Virus 1-Step Master Mix (Life Technologies) and 1 μl Primer/Probe mixture29,30 ...
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bioRxiv - Microbiology 2022Quote: ... the reactions with Taqman Fast Virus 1-step master mix (ThermoFisher scientific) were half of the recommended 20 μl volume ...
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bioRxiv - Microbiology 2023Quote: ... 10-fold dilutions of each virus in DMEM + 1% antibiotic/antimycotic (Gibco) were added to a monolayer Vero E6-TMPRSS2-T2A-ACE2 cells for 1 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Virus samples at variable dilutions (1:10 to 1:40,000) were mixed with FluoSphere (Invitrogen) beads (170nm ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 37°C to OD600 0.6 and protein expression induced with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG; ThermoFisher Scientific) for 4 hr at 30°C (3CL-pro and RBD) ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... Full-length Tat was PCR amplified with Phusion high-fidelity DNA polymerase (Thermo Scientific, Waltham, MA, USA). The amplified PCR products were digested with BamHI and HindIII and inserted into a EGFP-C1 vector (Clontech ...
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bioRxiv - Microbiology 2021Quote: PBMCs from HIV-negative donors were CD8-depleted (Invitrogen Cat# 11147D) and activated with 0.5μg/mL purified PHA (Remel Cat# R30852801 ...
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bioRxiv - Immunology 2022Quote: HIV-infected cells were stained with LIVE/DEAD AQUA viability (Invitrogen) for 30 mins at RT ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Neuroscience 2022Quote: ... and run by Eurofins on the human Clariom D transcriptomic array (Thermo Fisher) using the GeneChip WT Plus labelling kit reagent ...
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bioRxiv - Genomics 2020Quote: ... Human PDCD1 or PDL1 cell surface protein staining was analyzed using anti-PD-1 (ThermoFisher clone MIH4) or anti-PD-L1 (ThermoFisher clone MIH1 ...
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... Plasma HIV-1 RNA was extracted using PureLink® Viral RNA/DNA Mini Kit (Invitrogen, Thermo Fisher Scientific, USA) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Plasma HIV-1 RNA was extracted using PureLink® Viral RNA/DNA Mini Kit (Invitrogen, Thermo Fisher Scientific, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: HIV-1 RNA from the QVOA supernatants was isolated using PureLink™ Viral RNA/DNA Mini Kit (Invitrogen, #12280050) and RT-PCR was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... An aliquot of 50 ng of purified HIV-1 env DNA was used to clone into the pcDNA 3.1D/V5-His-TOPO vector (Invitrogen) and MAX Efficiency Stlb2 competent cells (Life Technology ...
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bioRxiv - Microbiology 2024Quote: ... and the HIV-1 latently infected CD4+ CEM cells ACH-2 (A3.01 cell integrated HIV-1 proviral DNA) were cultured in complete RPIM640 (Gibco) containing 10% FBS and 1% penicillin/streptomycin at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: Glial-Derived Neurotrophic Factor (GDNF) Recombinant Human Protein (ThermoFisher, #PHC7044)
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bioRxiv - Neuroscience 2020Quote: Brain-Derived Neurotrophic Factor (BDNF) Recombinant Human Protein (ThermoFisher, #PHC7074)
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bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and pre-coated vitronectin recombinant human protein (ThermoFisher Scientific #A14700) at 0.5 μg/cm2.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Human paraoxonase1 (PON1) recombinant protein was purchased from Thermo Fisher Scientific Inc ...
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bioRxiv - Systems Biology 2024Quote: ... FGF Basic (aa 10 155) Recombinant Human Protein (Gibco #PHG0021), 1% Penicillin-Streptomycin Solution and 1% L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: ... or human TGFβ1 recombinant protein (10ng/mL)(ThermoFisher Scientific, PHG9204) for 24 hours followed by addition of lipopolysaccharides (LPS ...
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bioRxiv - Pathology 2023Quote: In vitro TAT-Cre recombinase was performed by diluting 0,05 µg or 0.1µg or 0.2µg of homemade TAT-Cre in a final volume of 10µL of Opti-MEM reduced serum medium (GIBCO). Next ...
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bioRxiv - Microbiology 2023Quote: ... Tat mRNA was then synthesized using the PCR amplified DNA using the MEGAscript T7 Transcription Kit (Thermo Fisher). The resulting RNA was purified using the RNEasy Plus Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... using the DSB-X Biotin Protein Labeling Kit (ThermoFisher D-20655). Nephron progenitors bound to these beads were isolated as previously published (Hemker et al. ...
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bioRxiv - Cell Biology 2021Quote: ... S1 D (PageRuler Plus Prestained Protein Ladder from Thermo Scientific, 26619). SDS-PAGE was performed for each septin prep.
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
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bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
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bioRxiv - Microbiology 2022Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
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bioRxiv - Microbiology 2023Quote: ... MCC virus-positive MKL-1 cells were cultured in 1x RPMI (ThermoFisher Scientific) supplemented similarly as stated above ...
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Microbiology 2020Quote: ... HIV negative donors and depleted of CD8+ T cells using Dynabeads (ThermoFisher). Approximately 30 x 106 cells were placed in 30ml of R10 medium ...
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bioRxiv - Genetics 2019Quote: ... human COT-1 DNA (Invitrogen) and biotin-labeled mouse COT-1 or minor satellite DNA (a gift from Dr ...
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bioRxiv - Genetics 2021Quote: ... human COT-1 DNA (ThermoFisher) to detect HSA21 and biotin-labeled I-EGFP-I-loxP-3’HPRT (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Human Cot-1 DNA (Invitrogen), 3M NaAc and 100% cold EtOH ...
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bioRxiv - Genomics 2020Quote: ... Samples passing quality control were analyzed using the human Clariom D Assay (ThermoFisher Scientific) and analyzed using Transcriptome Analysis Console (ThermoFisher Scientific ...