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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and virus amplification in Spodopetera frugiperda 9 (Sf9) cells (ThermoFisher) was performed using standard procedures and as described in Zeqiraj et al ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
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bioRxiv - Immunology 2024Quote: ... Virus inoculum was prepared in DMEM (Thermo Fisher Scientific, 119905040) supplemented with 2% FBS and allowed for 1 hour at 37°C for virus adsorption onto cells ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Immunology 2021Quote: ... α-zona occludens-1 (ZO-1) protein (1:200, Invitrogen, 61-7300) and the lectins Ulex europaeus agglutinin - Atto488 conjugated (UEA ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Microbiology 2020Quote: ... Cell-free HIV-RNA was extracted as previously described (87) and used for cDNA synthesis using SuperscriptIV (Invitrogen) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... 600 nM of HIV gag reverse primer and 5 U of Platinum Taq DNA polymerase (Life Technologies; USA). In the 2nd round PCR 5’ LTR region of HIV-1 was amplified using 1 μl of 1st round PCR amplicon as a template ...
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bioRxiv - Biophysics 2022Quote: HIV pseudoviruses were produced as described before by transfection of HEK 293T cells (ATCC) with Lipofectamine 2000 (Invitrogen) and the following amounts of plasmids per 10 cm dish ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... Antibody-bound complexes were immunoprecipitated with Novex DYNAL Dynabeads Protein G (Invitrogen, cat. no. 10-003-D) or protein A/G agarose beads (EMD Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... 500 µg of lysate was immunoprecipitated with 40 µL Protein A dynabeads (Fisher Scientific, 10-001-D).
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bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-human IL6 Ab concentrations were quantified using the Quant-it protein assay kit (Life Technologies). Ab conjugates were validated on 10% TBE urea denaturing gels and validated on an agarose gel (Supplementary Figure S8) ...
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bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
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bioRxiv - Genomics 2022Quote: ... human preadipocytes in a 6-well plate were transfected with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: Human ACE2 fusion proteins were obtained from transient transfection using the Expi293TM system (Thermo Fisher Scientific). All plasmids used in this study were generated by ATUM Bio and transient transfection was carried out following the manufacturer’s protocol for the ExpiFectamineTM 293 transfection kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Protein A and Protein G coupled magnetic beads (mixture 1:1, Dynabeads, Thermo Fisher, 10002D and 10004D) were pretreated with BSA (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen) for 3 h at 4°C ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... were crosslinked to a 1:1 mixture of Dynabeads protein A and protein G (Thermo Fisher Scientific) with DMP and incubated with the supernatant at 4°C for 2 hr ...
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bioRxiv - Bioengineering 2022Quote: ... A 40 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for an additional 4 h at 4°C on a rotator (400 rpm) ...
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bioRxiv - Immunology 2023Quote: ... A 20 μL aliquot of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) was added and incubated for 4 h at 4°C with rotation (50 rpm) ...
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bioRxiv - Developmental Biology 2021Quote: ... where EGF and noggin were replaced with human forms with 10 ng ml−1 human EGF (Invitrogen, PHG0311), 100 ng ml−1 human noggin (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were: HuC/D (ThermoFisher # A-21271; 1:250 dilution) and TuJ1 (BioLegend # MMS-435P ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then counterstained with DAPI (1:5000, Invitrogen, D-1306) and mounted with Fluoromount Gold and allowed to dry for 1 day.
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bioRxiv - Molecular Biology 2020Quote: ... Isopropyl β-D-1-thiogalactopyranoside (IPTG) was purchased from Fisher Scientific. Primers used to make mutant constructs are included in Table S1 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-HuC/D (Thermo-Fisher Scientific A-21271, 1:100), and chicken anti-TUJ1 (Aves TUJ ...
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bioRxiv - Molecular Biology 2023Quote: ... were induced with 1mM IPTG (Isopropyl β-d-1-thiogalactopyranoside, ThermoFisher) at 25°C for approximately 16 hours ...
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bioRxiv - Biophysics 2022Quote: ... high glucose D-MEM with 1% penstrep (Penicillin Streptomycin, Fisher Scientific) supplemented with 10% Bovine Calf Serum (BCS ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-HuC/D monoclonal antibody (Invitrogen A21271, 1:300 dilution), mouse anti-4c12 monoclonal antibody (gift from Dr ...
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bioRxiv - Developmental Biology 2023Quote: ... cell clusters were incubated in Day (D)1 medium [RPMI (Invitrogen) containing 0.2% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... + CHIR99021 (SelleckChem; 7.5 µM)) and D -1 day (Neurobasal media (Gibco) + B27 supplement (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded in DMEM 1 g/L D-glucose (Gibco) with 10% FBS and 1% P/S ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-HuC/D (Elavl3/4) (1:500, Invitrogen A21271) in PBST overnight at 4 °C in a humidified chamber ...
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bioRxiv - Bioengineering 2024Quote: ... mouse anti-HuC/D (Thermo Fisher Scientific, Cat#: A21271; 1:150) and goat anti-Otx2 (R&D systems ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
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bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...