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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Human Chemokine Like Factor Superfamily 6 CKLFSF6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 ng/ml bFGF recombinant human protein (Thermo Fisher Scientific, USA). From day 6 until day 12 the medium was changed once in two days with the addition of 1 mM valproic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Human alpha-1 PDX (alpha1-PDX) recombinant protein was purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... 5 μg of FGFb Recombinant Human Protein and 1% Gluta-MaxTM (Gibco) in flasks coated with Matrigel Matrix Basement Membrane (Corning ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 ng/mL Human TGF-β 1 recombinant protein (Cat# PHG9204, Gibco) and 1 μg/mL L-Ascorbic acid 2-phosphate (Cat# A8960 ...
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...
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bioRxiv - Biochemistry 2020Quote: Receptor and chemokine baculovirus stocks were produced using the Bac-to-Bac Baculovirus Expression System (Invitrogen). Briefly ...
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bioRxiv - Physiology 2024Quote: Brain homogenates from all mice were assessed with a 36-plex Procartaplex cytokine/chemokine panel (ThermoFisher) as previously reported [14 ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected and diluted 1:6 for BCA protein quantification (ThermoFisher #23227) and performed according to manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: ... DNA-protein complexes were separated on non-denaturing 6% DNA retardation gels (Invitrogen, #EC63655BOX) at 100V constant voltage for 50 min ...
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bioRxiv - Developmental Biology 2020Quote: ... An aliquot of chromatin (6 ug) was precleared with protein A agarose beads (Invitrogen). Genomic DNA regions of interest were isolated using 12 ug of antibody against H3K27me3 (Millipore ...
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bioRxiv - Zoology 2021Quote: ... The protein-DNA complexes were separated using a 6% DNA retardation gel (Invitrogen, EC6365BOX) in 0.5% TBE buffer and transferred onto nylon membranes ...
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bioRxiv - Plant Biology 2023Quote: ... DNA-protein complexes were separated in a 6% (w/v) retardation gel (EC6365BOX, Invitrogen). DY682 signal was detected using the Odyssey infrared imaging system from LI-COR.
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bioRxiv - Genetics 2022Quote: ... A mix of 6 ul of nick translated RNA probe with 12ul of Human Cot-1 (ThermoFisher) and 2ul of salmon testes (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... mature factor X (FX) protein preceded by the prothrombin signal sequence and propeptide were purchased from GenArt (Thermo Fisher Scientific). N-glycosylation acceptor sites were introduced successfully at modelled surface exposed sites of publicly available crystal structure of FXa (PDB id 1FAX and residues 60 ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2023Quote: ... Internalization of Alexa Fluor 488-conjugated human α-syn PFFs (1 μM) and epidermal growth factor (EGF; 4 μg/mL; Thermo Fisher Scientific) were assessed similarly ...
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bioRxiv - Immunology 2023Quote: ... or VHHASC with a C-terminal HA tag or C-terminal EGFP fusion under the control of the human elongation factor-1 α promoter (pEF1α) were generated using lentiviral vectors constructed by Gateway cloning (Thermo Fisher Scientific) using vectors modified from pRLL (a kind gift of Susan Lindquist ...
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bioRxiv - Developmental Biology 2021Quote: ... iβ-like clusters were labeled with the long-term tracer for viable cells CFDA (Invitrogen) before transplantation ...
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bioRxiv - Microbiology 2022Quote: ... The ISG15-like systems were cloned into an arabinose-inducible plasmid (pBAD, Thermo Fisher #V43001). The cloned vector was transformed into E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the gel-like pellet was resuspended in 200.0 μL of nuclease-free water (Ambion). The DNA concentration of the preparation was measured with the Qubit® dsDNA HS Assay Kit (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2021Quote: ... 25ng/mL epidermal growth factor (Invitrogen) and mice fibroblast growth factor 7 (R&D system) ...
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bioRxiv - Systems Biology 2022Quote: ... TNFα (tumor necrosis factor-alpha) (ThermoFisher), PGE2 (prostaglandin E2 ...
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bioRxiv - Biochemistry 2023Quote: ... Coagulation factor XII (Invitrogen, RP-43076), von Willebrand factor (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and fibroblast growth factor (FGF) (Gibco). Expanded rOPCs were seeded in 96-well AA plates at a density of 20,000 cells per well in differentiation medium ...
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bioRxiv - Biochemistry 2023Quote: ... von Willebrand factor (Invitrogen, RP-43132), transferrin (R&D systems ...
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bioRxiv - Neuroscience 2024Quote: ... expressing reprogramming factors (CytoTune, Life Technologies). All reprogramming and assessment of pluripotency was performed by Sampled ...
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bioRxiv - Biochemistry 2019Quote: ... LRRK2 (G2019S) Recombinant Human Protein (residues 970-2527 #10499963) was purchased from Invitrogen and all additional kinases used in the in vitro screen were from MRC PPU Reagents and Services – including TAK1 (1-303) ...
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bioRxiv - Neuroscience 2019Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2020Quote: Serum cytokine and chemokine analysis was performed using a magnetic bead-based plex mouse panel (Thermo fisher) targeting the indicated molecules ...
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bioRxiv - Microbiology 2021Quote: ... Cytokines and chemokines were measured from irradiated sera using a magnetic Cytokine 29-Plex Monkey Panel (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: A multiplex bead-based immunoassay (Cytokine & Chemokine 26-Plex Mouse ProcartaPlex™ Panel 1, Thermo Fisher Scientific) was used for simultaneous quantification of the following cytokines/chemokines ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cancer Biology 2021Quote: Cells were stimulated for a total of 6 hours with human T-activator anti-CD3/CD28 Dynabeads (ThermoFisher) stimulation with GolgiStop solution being added for a total of 5 hours block intra-cellular protein transport (BD Bioscience) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... hiPS cells were maintained in 6-well plates coated with recombinant human vitronectin (VTN-N, Life Technologies, A14700) in Essential 8 medium (Life technologies ...
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bioRxiv - Immunology 2022Quote: ... The resulting monocytes were then differentiated with 50 ng/ml human M-CSF (Miltenyi) for 6 days in Iscove’s modified Dulbecco’s medium (Gibco) containing 5 % fetal calf serum (CAPRICORN ...