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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Human Chemokine Like Factor Superfamily 6 CKLFSF6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Lung cells collected on day 2 and day 6 post-infection was cultured overnight and supernatants collected were assayed for cytokines and chemokines using the multiplex magnetic bead-based kit (ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: ... cell supernatants were collected for cytokine/chemokine analyses and cells were stained with Fixable Aqua Dead Cell Stain Kit (Invitrogen/Thermo Scientific) for determination of cell surface expression of co-stimulatory molecules by FACS (FACSCanto II ...
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bioRxiv - Immunology 2021Quote: ... was used to quantitate genomic viral RNA and mRNA levels of cytokines and chemokines (Power-Up SYBR-green master mix, Life Technologies) in a Quant-Studio 5- or 6-flex.
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bioRxiv - Immunology 2022Quote: ... The levels of cytokines in the serum samples were determined by Cytokine & Chemokine Convenience 36-Plex Mouse ProcartaPlex™ Panel 1A (ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Genomics 2020Quote: ... Human PDCD1 or PDL1 cell surface protein staining was analyzed using anti-PD-1 (ThermoFisher clone MIH4) or anti-PD-L1 (ThermoFisher clone MIH1 ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Cell Biology 2021Quote: Peak lists were searched against the complete human proteome (UniProt release 2014_08, with 68.049 proteins) using SEQUEST (Proteome Discoverer 1.3, ThermoFisher) as search engine ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: The dual pri-miRNA expression plasmids were transfected to human embryonic kidney (HEK) 293 cells in 6-well plates using the Lipofectamine 3000 reagents (Thermo Fisher Scientific) following standard procedures ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs were plated onto 6-well dishes coated with Matrigel (Thermo-Fisher Scientific; Waltham, MA, USA; Cat. No. 08-774-552) in mTeSR1 medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA/protein complexes were then electrophoreticed by 6% native polyacrylamide gel and transferred to nylon membrane (Thermo Fisher Scientific). RNA was cross-linked with a UV lamp at a distance of 0.5 cm from the membrane for 2 min ...
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bioRxiv - Genetics 2019Quote: ... The chromatin with the antibodies was incubated with 6 μl of Dynabeads Protein G (Thermo Fisher Scientific, Cat# 10003D) for one hour ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant containing secreted pro-HlyA protein was collected by centrifugation (Thermo Scientific, Sorvall RC 6+, SLA-3000 (Rotor), 10,000 × g for 30 min at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we first extracted total RNA from pools of tissue derived from ten like individuals using TRIzol Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... it was necessary to use a fluorescent DNA quantification method with high sensitivity like the Qubit dsDNA HS kit (Invitrogen). Quality assessment ...
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bioRxiv - Biochemistry 2020Quote: ... Further sub-cloning of ZmGl2 and ZmGl2-like sequences for plant expression experiments were performed using LR Clonase II Enzyme Mix (Invitrogen), using pEarleyGate100 vector (Earley et al. ...
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bioRxiv - Neuroscience 2022Quote: ... transfected (psDsRed-2Mito) striatal-like neurons in coverslips were washed with Hanks’ Balanced Salt solution (HBSS) (#14025, Thermo Fisher Scientific). Mitochondrial movement videos were acquired on a Zeiss Cell Observer Spinning Disk System (Carl Zeiss Microscopy) ...
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bioRxiv - Microbiology 2022Quote: ... WTC hiPSCc were passaged as small clumps for maintenance or single cell-like suspension for cardiac differentiation using Versene (Gibco) and mTeSR Plus supplemented with 10 μM Y-27632 (Tocris) ...
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bioRxiv - Bioengineering 2020Quote: ... the cell instructive LEGO-like scaffolds were prepared by loading microgels supplemented with 10 µg/mL of rhVEGF165 (Invitrogen, ThermoFisher) and PDGF-BB (R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... the cell instructive LEGO-like scaffolds were prepared by loading microgels supplemented with 10 µg/mL of rhVEGF165 (Invitrogen, ThermoFisher) and PDGF-BB (R&D Systems ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the monolayers containing a mixture of mononucleated CTB-like cells and multinucleated STB cells were partially dissociated using StemPro Accutase (A1110501, Gibco) for 8-12 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were chosen for this study due to the absence of endogenous ASIC-like currents [53] and were grown using standard procedures in the following medium: Ham’s F-12 Nutrient Mixture (Life Technologies), 10 % fetal bovine serum (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... ATCC CCL-2) cells and 3T3-L1 (murine embryo fibroblast-like cells, ATCC CL-173) were grown in DMEM (Gibco) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... E and S1 exposure were conducted in the presence or absence of neutralizing antibodies against toll-like receptor 2 and 4 (TLR2 and TLR4) antibodies (ThermoFisher Scientific Cat ...
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bioRxiv - Plant Biology 2022Quote: ... Like samples were combined into a single tube then quantified by high sensitivity DNA Qubit (ThermoFisher scientific, Cat. No. Q32854).
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... For both fungi, first we obtained fresh blastospores (i.e., yeast-like single cells) suspended in Grace’s Insect Medium (Gibco, Thermo Fisher Scientific) supplemented with 2.5% FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... resuspended in extracellular-like Ca2+-free buffer (120mM NaCl; 5mM KCl; 1mM KH2PO4; 0.2mM MgCl2·6H2O (Fisher Scientific #M35-500); 0.1mM EGTA ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from cryo-pulverized liver tissue and hiPSC-derived hepatocyte-like cells using Trizol (Thermo Fisher Scientific) and 1 ug RNA was reverse-transcribed using SuperScriptTM IV VILOTM Master Mix (#11756050 ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
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bioRxiv - Physiology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cell supernatants were collected for cytokine/chemokine analyses and cells were stained with Fixable Aqua Dead Cell Stain Kit (Invitrogen/Thermo Scientific) for determination of cell surface expression of co-stimulatory molecules by FACS (FACSCanto II ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... Colon lysates and serum samples were subjected to a bead-based multiplex assay (Cytokine & Chemokine 36-Plex Mouse ProcartaPlex Panel 1A, Invitrogen, Waltham, MA) performed on a Luminex 200 platform according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The level of IL-1β in microglia culture supernatants was measured by Procartaplex Mouse Cytokine and Chemokine kit (cat no: EPXR260-26088-901, Thermo Fisher Scientific) using the MAGPIX plate reader according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... The resulting conditioned medium was collected and assessed for inflammatory cytokine and chemokine concentrations using a ProcartaPlex multiplex immunoassay kit (Thermo Fisher Scientific). The cells were lysed and assessed for NF-κB activity with an Illumination Firefly Luciferase Enhanced Assay Kit (Gold Biotechnology) ...
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bioRxiv - Immunology 2023Quote: ... a Luminex assay using the ProcartaPlex™ Mouse Cytokine & Chemokine Panel 1 36-plex kit (#EPXR360-26092-901, ThermoFisher Scientific, Waltham, MA) was performed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Mouse lung homogenates were collected for Luminex analysis using the Cytokine & Chemokine 36-Plex Mouse ProcartaPlexTM Panel 1A (ThermoFisher, # EPX360-26092- 901). Samples were incubated with fluorescent-coded magnetic beads pre-coated with respective antibodies in a black 96-well clear-bottom plate overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: Analysis of cytokines and chemokines from sera and lungs homogenates collected 5 dpi and 5 dpc were conducted using the ProcartaPlex TM Mouse Cytokine & Chemokine Convenience Panel 1 26-plex (Thermo Fisher Scientific) following the manufacturer’s specifications ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Developmental Biology 2019Quote: ... pH=6 (ThermoFisher). Sections were then blocked for 1h at RT with 10% normal donkey serum (Chemicon International Inc ...
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bioRxiv - Biochemistry 2024Quote: ... or 6% (Thermofisher) tris-glycine SDS-PAGE gels then transferred onto PVDF membranes using the Transblot rapid transfer machine (BioRad) ...
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bioRxiv - Genomics 2020Quote: Small-interfering (si)RNA knockdown of LOC157273 was performed on primary human hepatocytes from donor Hu8200 (genotype G/G at rs4841132) plated into collagen-coated 6-well plates (ThermoFisher Scientific #A11428-01). Cells were incubated in complete Williams’ E medium and transfected with siRNA (50 nM ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...