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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 100 nM of each primer and 10 μl 2x SYBR® Green PCR Master Mix (Applied Biosystems™). qPCR cycles consisted of the initial cycle of 50°C for 2 minutes and the second cycle of 95°C for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time quantitative PCR was performed using 2x Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific) to amplify 90 bp target with LINE primers (final concentration 200 nM) ...
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bioRxiv - Plant Biology 2020Quote: ... the following reagents were used: 25 uL DreamTaq PCR Master Mix (2X) (Thermo Fisher Scientific, Grand Island, NY), 1.0 uL forward primer at 10 uM ...
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bioRxiv - Cancer Biology 2021Quote: ... We perform amplification using the Maxim SYBR Green qPCR Master Mix reagent (2X) (Applied Biosystems, Carlsbad, CA, USA), following the protocol (1.5 μM of each primer ...
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bioRxiv - Neuroscience 2020Quote: ... 2x SYBR master mix (Thermoscientific) were used and the reaction was run in Thermocycler (Applied Biosystems ViiA 7) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Gene expression was analysed with TaqMan Assays on Demand using 2X TaqMan Gene Expression Master Mix (Life Technologies) using a 1:10 dilution of the cDNA product ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... QPCR was done using RealQ Plus 2x Master Mix Green (Ampliqon, #A324402) on a QuantStudio 5 (Applied Biosystems) machine ...
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bioRxiv - Evolutionary Biology 2021Quote: The qPCR reactions were generated using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Thermo Fisher Scientific, Germany) and the amplifications were conducted on ABI 7500 real-time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... cDNA reaction was adjusted to 500 ng/μl and used for PCR in Master Mix 2x (Thermo Scientific), using a 3PRIME Thermal Cycler (TECHNE ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR was performed using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Thermo Fisher Scientific, Waltham, MA USA) by the Thermo Fischer Scientific QuantStudio 7 Flex Real-Time PCR Instrument ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μL qPCR reactions were carried out using 2x PowerUp SYBR Green Master Mix (Fisher Scientific cat# A25776), 800 nM primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time polymerase chain reaction (PCR) analysis was performed using 2x SYBR Green Master Mix (Thermo Fisher Scientific) on an ABI Prism 7900 Sequence Detection System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... RealQ Plus 2X Master Mix (Amplicon) was used for qPCR amplification on a QuantStudio 6 Flex (Applied Biosystems). For Western Blot ...
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bioRxiv - Developmental Biology 2024Quote: ... Reactions with cDNA samples were prepared using a mixture of 2X SYBR Green Master Mix (Applied Biosystems, #4309155) and specific primers designed based on previous studies71 ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were amplified using appropriate primer pairs (Tables S2 and S3) and Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific). Linear fragments were purified on agarose gels ...
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bioRxiv - Neuroscience 2020Quote: ... 5 uL Gene Expression Master Mix or Fast Advanced Master Mix (ThermoFisher), and 2 uL diluted pre-amplified cDNA ...
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bioRxiv - Immunology 2024Quote: ... The PCR master mix (TaqPath ProAmp Master Mix; Thermo Fisher Scientific, #A30866) was combined with taqman probes for Ep300 (Mm00625535_m1 ...
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bioRxiv - Biochemistry 2023Quote: ... PCR Master mix (PowerUp™ SYBR™ Green Master Mix, Applied Biosystems) was added to each well along with the specific primer pair for each gene ...
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bioRxiv - Developmental Biology 2021Quote: ... using Overlap Extension PCR primers and Phire Green Hot Start II PCR Master Mix (ThermoFisher). After agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... Resulting cDNA was analysed using TaqMan Universal Master Mix II (Life Technologies, Carlsbad, CA, USA). Gene expression was determined by the comparative ΔΔCT method ...
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bioRxiv - Cell Biology 2021Quote: ... Duplicate quantitative PCR assays were run on StepOne Real Time system from Applied Biosystems with TaqMan Universal Master Mix II and specific probes from assay on demand (Applied Biosystems). Three house-keeping genes (ACTIN B ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR (qPCR) was performed using the TaqMan Universal Master Mix II with UNG (ThermoFisher) in combination with the primers and probes listed in Suppl ...
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bioRxiv - Cell Biology 2021Quote: ... Resulting cDNA was analysed using TaqMan Universal Master Mix II (Life Technologies, Carlsbad, CA, USA) as well as corresponding Taqman probes ...
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bioRxiv - Immunology 2023Quote: ... and amplified for sequencing by nested PCR using Platinum SuperFi II Master Mix (Thermo Fisher). The DNA was initially amplified with primer pairs specific for each gene (IGHG1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µl primer and 10 µl TaqMan Universal Master Mix II no UNG (Thermofisher #4440040). TaqMan primer probes were purchased from Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... Amplifications were performed using TaqMan Universal Master Mix II (catalogue no. 4440038, Applied Biosystems, USA). Mastercycler RealPlex2 (Eppendorf ...
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bioRxiv - Neuroscience 2023Quote: ... Universal Master Mix (Invitrogen), and 200 nM of forward and reverse primers in a final reaction volume of 20 μl ...
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bioRxiv - Microbiology 2020Quote: ... cDNA and gDNA samples were quantified using 25 µ L reactions with 2x SYBR PowerUP Green Master Mix (Invitrogen) analyzed on a QuantStudio 3 (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... in a total volume of 25 μl containing: 2x Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific), 1 µL of cDNA and 0.3 µM of each primer ...
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bioRxiv - Neuroscience 2021Quote: RT-qPCR was carried out with PowerUp™ SYBR™ Green Master Mix (2x) (Thermo Fisher Scientific; Monza, Italy) for the septum and with SsoAdvanced™ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Zoology 2019Quote: ... Each PCR reaction was run in triplicate and included 5 μl of 2x Platinum Hot Start Master Mix (Invitrogen), 0.5 μl of 10 μM primers ...
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bioRxiv - Cell Biology 2019Quote: ... RT-qPCR reaction was performed using 5 ng of cDNA and 2x SYBRGreen master mix (Thermo Fisher Scientific, USA) on a BioRad thermocycler (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR was conducted in triplicate in a total of 20μL using PowerUp 2x SYBR Green master mix (Applied Biosystems), with a 60°C extension temperature ...
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bioRxiv - Zoology 2019Quote: ... All amplifications were performed at 25 μL volumes containing 12.5 μL 2X DreamTaq PCR Master Mix (Thermo Fisher Scientific), 1 μM forward primer ...
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bioRxiv - Genetics 2019Quote: PCR reaction for the V1016I mutation contained 10 μL of 2X PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), 0.5 μL of each primer (50 pmol/μL) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR reaction was then set up in (20μl) as follows: 2x Flash Phusion PCR Master Mix (Thermo Fisher, US) (20μl) ...
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bioRxiv - Neuroscience 2020Quote: ... Real time RT-PCR reaction was carried out using Syber green 2X Universal PCR Master Mix (Applied Biosystems, USA) in ABI Prism 7500 sequence detection system ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture was prepared using the Power SYBR Green PCR Master mix 2X kits (Applied Biosystems, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... The taqman based q-RT-PCR was performed using the TaqMan Fast Universal PCR Master Mix (2X, Applied Biosystems) and ‘Fam (Fluorescein ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR reaction contained 20X TaqMan™ probe and 2X TaqMan™ Fast Advanced Master Mix (Thermo Scientific) in a total of 10 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... Each reaction contained 1X final concentration of SYBR Green PCR Master Mix 2X (Applied Biosystems, Foster City, CA, USA), 50 nM forward and reverse primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed in 50 mL of volume containing 25 mL of 2X DreamTaq Green PCR Master Mix (ThermoFisher), 2.5 mL of 10 mM forward and reverse primers ...
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bioRxiv - Physiology 2023Quote: ... PCR master mix solution included (per sample values): 16.6 μL of 2X Phusion HiFi Mastermix (Thermo Scientific Catalog #F531s), 14 μL of DNase/RNase free water ...
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bioRxiv - Neuroscience 2023Quote: ... The RT-qPCR was performed using the Promega® 2x Master Mix and the QuantStudio™ 6 (Applied Biosystems) for 40 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 µL of the cDNA product was added to 10 µL of 2X TaqMan Fast Advanced Master Mix (ThermoFisher) and 1 µL of 20X TaqMan Assay (including the TaqMan probe and PCR primer set ...
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bioRxiv - Genomics 2021Quote: ... and lines were genotyped using primers listed in Table S1 and Phusion High-Fidelity PCR Master Mix (with GC Buffer) (Thermo Scientific, F532). PCR products of candidate clonal lines showing predicted PCR band sizes in agarose gel electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
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bioRxiv - Neuroscience 2023Quote: ... with 12μL of master mix (TaqMan Universal Master mix fast, Life Technologies #4444964) and 8μL of lysate ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed using Platinum™ II Hot-Start Green PCR Master Mix (Thermo Fisher Scientific). Primers are specified In Table 1B ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... and the TaqMan® Universal Master Mix II with UNG on the QuantStudio 6 (ThermoFisher Scientific). Relative gene abundance was normalized to the mean expression of the house-keeping gene Actb ...