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Citations for Thermo Fisher :
51 - 100 of 5395 citations for Ferritin heavy chain FTH1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Plasmids pcDNA3.4 comprising heavy chains and light chains of antibodies were transiently expressed in ExpiCHO-S cells using the ExpiCHO™ expression system (Thermo Fisher). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were co-transfected by polyethylenimine-transfection to Expi293F™ cell (ThermoFisher, A14527). Cells were cultured at 36.5°C ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Heavy and light chain sequences of the b12 antibody were ordered at GeneArt (Thermo Fisher). All sequences were verified using Sanger sequencing.
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bioRxiv - Immunology 2021Quote: ... the corresponding heavy and light chain plasmids were chemically co-transfected into ExpiCHO cells (Gibco) and transiently expressed for 9 days ...
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bioRxiv - Immunology 2020Quote: ... Immunoglobulin epsilon (ε) heavy chain transcripts were amplified (StepOnePlus Real-Time PCR System, Applied Biosystems) using custom TaqMan gene expression kits (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... antibody heavy and light chain expression vectors were transiently transfected into Expi293 suspension cells (ThermoFisher). For the generation of the human IgG1 afucosylated Fc-domain variant ...
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bioRxiv - Microbiology 2021Quote: ... the plasmids with paired heavy chain (IgG, IgA1, IgA2) and light chain genes were co-transfected into Expi293™ expression system (Thermo Fisher Scientific) following the manufacturer’s protocol to produce recombinant monoclonal antibodies ...
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bioRxiv - Immunology 2020Quote: ... Four µg of the heavy chain and 4 µg of the corresponding light chain were mixed together in 375 µl Opti-MEM (Gibco, ThermoFisher Scientific, UK) before addition of polyethylenimine (PEI MAX (Polysciences ...
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bioRxiv - Immunology 2022Quote: ... 12μg heavy chain plasmid and 12 μg of light chain plasmid were added into 3ml of Opti-MEM™ (Thermo Fisher Scientific cat.# 31985070), after inverting ...
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bioRxiv - Immunology 2022Quote: ... Heavy and light chain plasmids were transfected into 293i cells using the Expifectamine kit (ThermoFisher A14524) as per manufacturer guidelines ...
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bioRxiv - Immunology 2019Quote: ... Heavy and light chain plasmids were co-transfected into 293T or ExpiCHO cells (Life Technologies A29133) for antibody expression.
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bioRxiv - Immunology 2022Quote: ... 20 μg of heavy and light chain expression plasmid DNA were diluted in OptiMEM (Life technologies) media containing polyetheleneimine (PEI ...
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bioRxiv - Biochemistry 2020Quote: ... Vectors for the heavy and light chains were transfected into HEK293F cells (Invitrogen, Grand Island, NY) using FectoPro according to the manufacturer’s instructions (Polyplus Transfection ...
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bioRxiv - Immunology 2020Quote: ... cDNA was used to amplify heavy and light chains using PlatinumTaq HotStart polymerase (Thermo Fisher Scientific) with 6% KB extender and optimized V gene-specific primer mixes (Kreer et al. ...
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bioRxiv - Immunology 2022Quote: ... The resulting plasmids of heavy and light chain pairs were co-transfected in Expi293F cells (Thermo Fisher) using Turbo293 transfection reagent (Speed BioSystems ...
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding Ig heavy and light chain pairs were co-transfected using Polyethylenimine into 293FreeStyle cells (Invitrogen). Supernatants were assayed for secreted rAb and for HA reactivity using the HA CBA described above ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were transfected into 96-well cultures of ExpiCHO cells (ThermoFisher Scientific, A29127) for microscale expression ...
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bioRxiv - Immunology 2021Quote: DNA constructs encoding anti-SCARB2 antibody heavy and light chain variable domains (clone JL278) were synthesized (ThermoFisher) and cloned into mammalian expression vectors containing a mouse IGHV signal peptide and IgG1 constant regions ...
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bioRxiv - Immunology 2021Quote: ... The constant regions of heavy (HC) and light chain (LC) were cloned separately into pcDNA3.4 (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were transfected with plasmids encoding Ig heavy and light chain pairs with ExpiFectamine (ThermoFisher Scientific). Monoclonal antibodies were purified from the cell supernatant using sepharose Protein A (Pierce).
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bioRxiv - Immunology 2023Quote: ... and were transiently transfected with an equimolar plasmid mixture of heavy and light chain using Expifectamine (Invitrogen). For a typical 100mL size transfection 100µg amount of total DNA and 270µL of lipofectamine were used ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the heavy and light chains were co-transfected into FreeStyle™ CHO-S™ cells (Invitrogen) in serum free FreeStyle™ CHO™ Expression medium ...
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bioRxiv - Immunology 2019Quote: ... The antibody variable heavy and light chain variable regions were PCR amplified using AmpliTaq360 Master Mix (Applied Biosystems). PCR products were purified (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... The Fab heavy and light chains of AI774 and AI878 were individually cloned into a pcDNA3 vector (Invitrogen). A 6x-His tag was added to the C-terminus of each Fab heavy chain sequence ...
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bioRxiv - Immunology 2020Quote: ... Expi293 cells were transfected with plasmids encoding Ig heavy and light chain pairs with Expi-Fectamine (ThermoFisher Scientific) and hmAbs were purified from the culture supernatants using Sepharose Protein A (Pierce).
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bioRxiv - Immunology 2022Quote: ... Heavy and light chain plasmids for each monoclonal antibody were co-transfected into Expi293F cells (Thermo Fisher Scientific). After incubating for 5 days at 37°C with 8% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: The codon-optimized for CHO variable light and heavy chains (02B11, atezolizumab, and durvalumab) were synthesized (ThermoFisher Scientific) and then cloned into pTRIOZ-mIgG2a/mkappa (κ ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgM u chain (Invitrogen, Thermofisher Scientific), for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgM u chain (Invitrogen, Thermofisher Scientific), for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Immunoblotting was performed using anti-FTH1 (MA5-32244; Thermo Fisher Scientific), anti-FTL (MA5-32755 ...
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bioRxiv - Biochemistry 2020Quote: ... using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50µg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50μg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The plasmids encoding the heavy and light chain genes were later transiently transfected in Expi293F cells (Thermo Fisher Scientific), and the recombinant mAbs were purified by Protein A/G affinity chromatography as reported45.
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bioRxiv - Immunology 2023Quote: ... Expi293F cells were co-transfected with heavy and light chain plasmid using Expifectamine (Thermo Fisher Scientific, Cat No. A14526) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were subsequently incubated with FTH1 (1:100, 710967, Thermo Fisher) at 4 °C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... mAbs were produced by transient transfection into Expi293F™ cells with equal amounts of paired heavy- and light-chain expression vectors using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (Thermo fisher). Five days post antibody expression ...
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Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... cells were spun down and washed with PBS then resuspended in 1mL blocking solution with either 1:500 Goat anti-Mouse IgM Heavy Chain Secondary Antibody conjugated to AlexaFluor 488 (Invitrogen), 1:500 Rabbit anti Mouse IgG (H+L ...
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bioRxiv - Immunology 2019Quote: ... 5μg filter-sterilized DNA (2.5μg each of heavy and light chain-encoding plasmids) was added to 1mL of Expi293 medium (ThermoFisher Scientific; A1435101) in a fresh tube ...
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bioRxiv - Immunology 2021Quote: For the antibodies, plasmids encoding heavy and light chains of antibodies (CR3022, and SR1-SR5) were co-transfected into Expi293F cells (ThermoFisher) according to the manufacturer’s instructions for expression of antibodies ...
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bioRxiv - Immunology 2021Quote: ... expression vectors for the heavy and light chain were co-transfected in the ExpiCHO cell line (Thermo Fisher Scientific, USA) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... paired heavy and light chain plasmids for each mAb were expressed in Expi293F cells (Cat# A14527, ThermoFisher Scientific, Waltham, MA) in a 96-well format ...
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bioRxiv - Immunology 2020Quote: Reverse transcription and subsequent PCR amplification of heavy and light chain variable genes were performed using SuperScript III (Life Technologies). First and second PCR reactions were performed in 50 ml volume with 5ul of reaction product using PCR mixture (SMART-Lifesciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... plasmids containing the light and heavy chain of 10C7 or a non-targeted control hIgG1 (cmAb) were transiently expressed in Expi293F suspension cells (ThermoFisher) using polyethylenimine reagent ...
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bioRxiv - Microbiology 2022Quote: ... The mNAbs were produced by transfection of Expi293 cells with equal amounts of matched heavy and light chain vectors using Expifectamine according to the manufacturer’s instructions (ThermoFisher Scientific). After 5 days of culture at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Knockdown of BubR1 and Dynein heavy chain (DHC) was achieved by transfecting 150 pM siRNA using Lipofectamine RNAiMax (Thermo fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: We generated immunoglobulin heavy chain (IGH) DNA libraries from complementary DNA generated from 10-500 ng of total RNA using Superscript III (Invitrogen) reverse transcriptase and random hexamer primers ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4′-6-diamidino-2-phenylindol) and Myosin Heavy Chain (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™). All images were taken using a confocal microscope (Zeiss LSM 780 Airyscan ...