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Citations for Thermo Fisher :
1 - 50 of 5395 citations for Ferritin heavy chain FTH1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Myosin Heavy Chain (ThermoFisher #Bt03273061), TERT (ThermoFisher #Bt03239211) ...
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bioRxiv - Neuroscience 2023Quote: ... we employed a pair of rabbit anti-human IgG heavy chain antibodies (Invitrogen) and HRP-conjugated goat α-human IgG kappa light chain antibodies (Thermo).
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bioRxiv - Bioengineering 2022Quote: ... and Myosin Heavy Chain (ThermoFisher #Bt03273061). Reactions were performed according to the manufacturer’s instructions on a Bio-Rad CFX96 Real Time System thermocycler ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-Clathrin Heavy Chain (1:1,000; Invitrogen); anti-goat-conjugated Alexa-488 (1:500 ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... and Myosin Heavy Chain (MHC) (ThermoFisher #Bt03273061). Reactions were performed using 2 uL of the prepared cDNA ...
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bioRxiv - Cell Biology 2020Quote: ... Clathrin Heavy chain (clone X22, ThermoFisher MA1-065), Lamin B (clone L-5 ...
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bioRxiv - Immunology 2022Quote: ... 11D5-3 single chain variable fragment (scFv)3 and the human heavy-chain-only FHVH3316 were codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific). Retroviral constructs encoding APRIL CARs ...
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bioRxiv - Cell Biology 2021Quote: ... with heavy chain and light chain plasmids using an ExpiCHO™ Expression System (Gibco). Cell culture was harvested after an 8-to 14-day incubation at 37C with humidified atmosphere of 8% CO2 with shaking ...
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bioRxiv - Bioengineering 2023Quote: ... ms anti cardiac myosin heavy chain (Invitrogen, MA1-26180); rb anti – myosin light chain (Cell Signalling ...
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bioRxiv - Cell Biology 2023Quote: ... or anti clathrin Heavy Chain (MA1-065, Thermo Fisher) at 1 μg mL−1 in PBS blocking buffer with 5% BSA and 0.01 % w/v saponin ...
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bioRxiv - Immunology 2023Quote: ... 20 µg of Heavy chain and Light chain plasmid was diluted in 1mL OptiMEM(Gibco) and 120 µL polyethyleneimine (1 mg/mL PEI ...
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bioRxiv - Bioengineering 2023Quote: ... smooth muscle myosin heavy chain (1:200; PA5-116524, Invitrogen), Piezo1 (1:25 ...
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bioRxiv - Immunology 2021Quote: Antibody heavy chain and light chain constructs were transiently expressed with the Expi293 Expression System (ThermoFisher). HC and LC plasmids were cotransfected at a 1:2.5 ratio with transfection reagent FectoPRO (Polyplus ...
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bioRxiv - Biochemistry 2019Quote: ... synthetic genes for light and heavy chains were obtained from Invitrogen GeneArt ...
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bioRxiv - Biophysics 2023Quote: ... Clathrin Heavy Chain siRNA (Human CLTC, sequence: GGUUGCUCUUGUUACG, ID: s475) and Negative Control#1 siRNA Silencer Select were purchased from ThermoFisher Scientific.
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bioRxiv - Immunology 2022Quote: ... heavy chain plasmid DNA and light chain plasmid DNA were diluted in 1.5ml Opti-Plex Complexation Buffer (Invitrogen) before mixing with 80µl ExpiFectamine 293 Reagent (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Four hundred micrograms of heavy chain and light chain plasmid were complexed with Expifectamine (ThermoFisher Cat No. A14526) and added to the Expi293 cells ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-Clathrin Heavy Chain (1:500, Thermo Fisher, clone X22). Before fixation ...
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bioRxiv - Cancer Biology 2021Quote: ... goat anti-mouse IgM (Heavy chain) Alexa Fluor 488 (ThermoFisher, A-21042). Immunofluorescence microscopy was performed using an Inverted Olympus IX83 microscope and Echo Revolve (Echo) ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Gibco) for expression ...
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bioRxiv - Immunology 2022Quote: Heavy and light chain plasmids were co-transfected into Expi293F cells (Gibco) for expression ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding paired Ig heavy and light chains were co-transfected into human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-clathrin heavy chain (AB X-22, MA1-065, Thermo Fisher Scientific) and rabbit anti-N-WASP (ab126626 ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding heavy and light chains were co-transfected into Expi293F cells (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Antibody heavy chains were prepared for conjugation using SiteClick™ azide modification (Invitrogen) of carbohydrate domains ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were labeled with mouse anti-clathrin heavy chain (Invitrogen, MAI-065) at 1:1,000 and rabbit anti-EPS15 (Cell Signaling D3K8R ...
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bioRxiv - Immunology 2021Quote: ... Purified heavy chain portion was incubated with 50mM biotin (Thermo Fisher, Cat#B20656) at 4-8 °C overnight for biotinylation ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Microbiology 2022Quote: ExpiCHO cells were transfected with equimolar amounts of pLM-M28 light chain and pLM-M28 heavy chain expressing plasmids according to the manufacturer’s protocol (Thermo Fisher). At 12 days post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding both the heavy chain and light chain for each antibody were co-transfected into FreeStyle 293-F cells (Invitrogen) using polyethylenimine ...
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bioRxiv - Bioengineering 2024Quote: ... c-myc epitope tag and the variable heavy chain from mAbCII linked to the variable light mAbCII chain was procured from GeneArt (ThermoFisher). This fragment was cloned into a lentiviral expression plasmid we have previously described (31 ...
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bioRxiv - Immunology 2022Quote: ... silencer select siRNA for human FtH1 and negative control siRNA (4392421 and 4390843 respectively, ThermoFisher Scientific) were used ...
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bioRxiv - Immunology 2024Quote: ... The resulting human chimeric heavy and light chain genes were separately cloned in pJET 1.2/blunt vector ( Thermo Fisher Scientific, Waltham, MA, USA). The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... and human H-chain ferritin (HHF) (UniProt: P02794) were custom synthesized by Integrated DNA Technologies and cloned into pcDNA3.1 (+) Zeocin (Invitrogen) using restriction cloning or Gibson assembly ...
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bioRxiv - Immunology 2021Quote: ... IgGs and 6xHis-tagged Fabs were expressed by transient transfection of paired heavy chain and light chain expression plasmids into HEK293-6E or Expi293 cells (Life Technologies). Fabs and IgGs were purified from transfected cell supernatants using Ni-NTA (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: A 250 mL culture of Expi293 cells at 3e6 cells/mL was transfected with 100 μg each of the heavy chain and light chain plasmids (pMAS478, pMAS481) in 20 mL of Opti-MEM (Life Technologies) supplemented with 200 μL of FectoPRO (Polyplus) ...
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bioRxiv - Immunology 2024Quote: Antibody heavy chain (HC) and light chain (LC) sequence pairs generated from 10X Genomics protocols were synthesized (Geneart, Thermo Fisher Scientific) and cloned into expression vectors containing an IgG1 HC or LC constant region ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Bioengineering 2021Quote: ... A rat-specific primary antibody for skeletal muscle myosin heavy chain (MA1-35718, ThermoFisher) was diluted 1:5 in TBS-NGS ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibodies used were anti-dynein heavy chain (1:250, Thermo Fisher Scientific) and anti-vinculin (1:1000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... by diluting heavy and light chain construct DNA in OptiMem serum-free media (Gibco) before the addition of and incubation with FectoPro (Polyplus Transfection ...
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bioRxiv - Immunology 2023Quote: ... Transfection with heavy and light chain plasmids was performed using the Expifectamine293 kit (Gibco) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... equal mass of heavy and light chain plasmids were transfected into Expi293F cells (Gibco) per manufacturer’s instructions and harvested 6 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... were transfected at cell densities between 8.0×105 to 1.0×106 cells/ml with equal masses of plasmids containing the heavy chain and light chain genes using FreeStyleTM MAX Reagent (ThermoFisher Scientific 16447100); both plasmids were diluted in OptiPROTM SFM (ThermoFisher Scientific 12309050) ...
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bioRxiv - Microbiology 2021Quote: ... The antibody production was conducted by co-transfection of the light chain and heavy chain sequences into Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific, A14635). The culture supernatant was collected at 7 days post-transfection ...
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bioRxiv - Immunology 2020Quote: ... Four µg of the heavy chain and 4 µg of the corresponding light chain were mixed together in 375 µl Opti-MEM (Gibco, ThermoFisher Scientific, UK) before addition of polyethylenimine (PEI MAX (Polysciences ...