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Citations for Thermo Fisher :
101 - 150 of 835 citations for Ebola Virus Envelope Glycoprotein GP1 Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...
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bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
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bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
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bioRxiv - Immunology 2023Quote: ... and H1N1/2009 influenza virus strains were prepared by overnight incubation of the virus strains with 0.1% v/v β propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1% PenStrep and transfected with plasmids encoding for the corresponding S glycoprotein (24 µg/dish) using lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Filaments extracted from gerbera were subjected to SDS-PAGE and then the Pierce™ Glycoprotein Staining Kit (ThermoFisher Scientific, USA) was used as directed ...
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bioRxiv - Developmental Biology 2022Quote: ... and 3.5 µg of pCAGGS SARS CoV-2 S Glycoprotein expression vector DNA (NR52310, BEI, USA) in a total of 10 ml of Opti-MEM media (Invitrogen). The day following transfection the media was changed to DMEM10 complete media and samples were placed at 37 °C and 5% CO2 for 48 h ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Molecular Biology 2022Quote: NiV prefusion glycoprotein and avi-tagged probes were expressed by transfection in 293 Freestyle (293F) cells (Thermo Fisher Scientific, MA) using Turbo293 transfection reagent (SPEED BioSystem ...
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bioRxiv - Molecular Biology 2021Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific) (29) ...
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing SARS-CoV-2 S-glycoprotein were seeded in 96 well plates for microscopy (Thermo Fisher Scientific) at 12’500 cells/well and the following day ...
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bioRxiv - Biochemistry 2021Quote: ... an equivalent of 1 μg glycoprotein digest was loaded onto an Acclaim PepMap RSLC C18 column (Thermo Fisher Scientific, Lithuania) and separated at a flow rate of 300nL/min using a gradient of 5% to 40% solvent B (80% acetonitrile with 0.1% formic acid ...
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bioRxiv - Microbiology 2020Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific). The wild-type SARS-CoV-2 spike (S ...
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bioRxiv - Microbiology 2021Quote: ... and glycol-proteins and the DNase/Proteinase K treated matrix samples with the Pro-Q Emerald 300 glycoprotein stain (ThermoFisher).
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bioRxiv - Microbiology 2019Quote: ... with TaqMan fast virus 1-step master mix (Thermo scientific) using AriaMx instrumentation (Agilent).
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bioRxiv - Cancer Biology 2019Quote: ... Virus was then resuspended in 1:1 Opti-MEM (Gibco) - HBSS ...
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bioRxiv - Microbiology 2019Quote: ... fibroblast-derived virus stocks were treated with TurboDNase (Thermo Fisher). For this ...
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bioRxiv - Immunology 2021Quote: ... Pelleted virus was resuspended in Opti-MEM (Gibco 31985-070) and stored at −80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The virus pellet was resuspended in 1 mL PBS (Gibco). For long term storage ...
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bioRxiv - Cell Biology 2021Quote: ... In brief the virus was diluted in DMEM (ThermoFisher Scientific) to achieve a multiplicity of infection (MOI ...
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bioRxiv - Pathology 2022Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix were used ...
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bioRxiv - Molecular Biology 2020Quote: ... and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) with 250 μM deoxy nucleoside triphosphate (dNTP ...
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bioRxiv - Microbiology 2019Quote: ... and Moloney murine leukemia virus reverse-transcriptase (MMLV-RT, Invitrogen). Subsequent PCR amplification performed in a thermocycler using specific primers ...
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bioRxiv - Genetics 2020Quote: Fibroblasts were infected by Sendai virus(Thermo Fisher Scientific, A16517) Transfected fibroblasts (approximately 1*105 cells per nucleofection ...
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bioRxiv - Microbiology 2021Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix (sequences shown in Table 1) ...
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bioRxiv - Immunology 2020Quote: ... the virus inoculum was replaced with fresh medium (MEM, Gibco) containing 1.25% Avicel® (FMC Biopolymer ...
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bioRxiv - Immunology 2021Quote: ... Virus was diluted in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) and maintained on ice prior to infection ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Immunology 2022Quote: ... Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and virus amplification in Spodopetera frugiperda 9 (Sf9) cells (ThermoFisher) was performed using standard procedures and as described in Zeqiraj et al ...
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bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific), primers ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus (M-MLV) Reverse Transcriptase (Invitrogen). One microgram of total RNA was retrotranscribed and the cDNA was diluted 1:10 to the quantitative PCR (qPCR) ...
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bioRxiv - Immunology 2024Quote: ... Virus inoculum was prepared in DMEM (Thermo Fisher Scientific, 119905040) supplemented with 2% FBS and allowed for 1 hour at 37°C for virus adsorption onto cells ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Microbiology 2022Quote: ... envelope plasmid (0.9 µg pMD2.G) and pLX304 carrying eGFP-LC3B (9 µg) using Opti-MEM (Fisher Scientific) and FuGENE HD transfection reagent (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... 6ug of psPAX2 viral packaging vector and 3ug pMD2.G viral envelope vector overnight with Lipofectamine 3000 (Invitrogen) according to manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The presence of glycoproteins was examined by staining the gels with SYPRO Ruby protein gel stain according to the manufacturer’s instructions (Thermo Fisher Scientific). Stained LPS and protein bands were visualized under UV light using an Azure c300 gel imaging system (Azure Biosystems).
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bioRxiv - Developmental Biology 2022Quote: ... 30 µg of pCAGGS SARS CoV-2 S Glycoprotein expression vector DNA (NR52310, BEI, USA) in a total of 10 mL of Opti-MEM media (Invitrogen, USA). One day after transfection the media was removed ...
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bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... ss-HA-Spike plasmids (HA tag at the N terminus with a Serine-Glycine linker between residues S13 and Q14 of S glycoprotein (Stewart et al., 2021)) were transfected using Lipofectamine 3000 (ThermoFisher Scientific), following the manufacturers guidelines ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 µg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Immunology 2022Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 μg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...