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Citations for Thermo Fisher :
1 - 50 of 835 citations for Ebola Virus Envelope Glycoprotein GP1 Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Cell Biology 2022Quote: ... and 2.16 μg of the vector expressing the VSV-G envelope glycoprotein (pMD2.G) were mixed with OptiMEM (Gibco) to a final volume of 400 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... plasmid encoding myc- and His-tagged full-length EBOV GP (Zaire ebolavirus isolate Ebola virus/H.sapiens-wt/SLE/2014/Makona-G3838) using Lipofectamine™ 3000 (Thermo Fisher Scientific Cat. Nr. L3000) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 μg of plasmid VSV-G encoding G glycoprotein of vesicular stomatitis virus(VSVG) using Lipofectamine 3000 Reagent (Invitrogen). 12 h later ...
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bioRxiv - Cancer Biology 2022Quote: Glycoproteins were isolated using a glycoprotein isolation kit (89804; Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Biophysics 2023Quote: ... Ebola GP expression plasmid kindly provided by Jochen Bodem) using Lipofectamine™ 3000 (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2021Quote: ... the 5’LTR sequence of GP1 (AT2TE23670) was cloned into the donor vector pDONR207 (Invitrogen) using primers PHG121 and PHG124 ...
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bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
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bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Tissues were incubated with mouse anti-P-glycoprotein C219 antibody at 3.6μg/mL (Glycoprotein Monoclonal Antibody C219, Cat. No MA1-26528, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A glycoprotein ladder (Candy Cane ladder; Invitrogen) served as positive control ...
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bioRxiv - Microbiology 2024Quote: ... along with a CandyCane glycoprotein ladder (Thermofisher). We additionally loaded the same two B ...
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bioRxiv - Systems Biology 2020Quote: ... and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375, Thermo/Invitrogen), according to the manufacturers protocol ...
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bioRxiv - Microbiology 2020Quote: ... GP was detected by western blotting with an anti-GP1 polyclonal rabbit serum described previously (22) followed by an Alexa-Fluor 680 dye conjugated anti-rabbit secondary antibody (ThermoFisher). Blots were imaged using the LI-COR Fc fluorescence imager (LI-COR ...
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Immunology 2021Quote: ... The lentiviral envelope vector pLP/VSVG (Invitrogen) and the packaging vector psPAX2 (Trono lab ...
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bioRxiv - Molecular Biology 2020Quote: ... and envelope plasmid in OptiMEM (Life Technologies) for 5 to 6 hours (PEI:total pDNA μg ratio 2:1) ...
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bioRxiv - Developmental Biology 2022Quote: ... or with Pierce™ Glycoprotein Staining Kit (Thermo Scientific) after separation.
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: Antibodies and antibody Fabs were produced as described previously.57 BG505 N332 SOSIP gp140 envelopes were expressed as previously described with minor modifications.58 Envelope production was performed with Freestyle293 cells (Invitrogen). On the day of transfection ...
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bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
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bioRxiv - Molecular Biology 2020Quote: ... and 260 ng pMD.G envelope plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25 µg envelope plasmid diluted in 100 µl optiMEM (Gibco) was mixed with 20 µl of PolyFect transfection reagent (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Immunology 2021Quote: ... The primary antibodies used were as follows: P-glycoprotein (1:1000, Invitrogen MA1-2652), Caspase-1 (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... VSV pseudotyped with EBOV glycoprotein was grown by infecting Vero cells (Ambion, Austin, TX) and then harvesting via filtration of the supernatant through 0.4 µM filters 24-30 hours after infection ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Biophysics 2022Quote: ... and pCMV-VSV-G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The gel was then stained using Pro-Q Emerald 300 staining for glycoproteins kit (Invitrogen) following the procedure described by the supplier.
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Immunology 2024Quote: ... The lentiviral envelope vector pLP/VSVG was purchased from Invitrogen (Invitrogen/ life technologies) and the packaging vector psPAX2 was a kind gift from Didier Trono (Addgene plasmid # 12260).
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bioRxiv - Immunology 2021Quote: ... spike glycoproteins were produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... envelope plasmid pIP/VSV-G (5μg) (ViraPower™ Lentiviral Packaging Mix, Thermo Fisher Scientific) and 5μg lentiviral expression constructs shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5µg psPAX2 packaging plasmid and 1µg pMD2.G envelope plasmid using Lipofectamine 2000 (Invitrogen). Lentiviral supernatant was collected ...