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Citations for Thermo Fisher :
251 - 300 of 835 citations for Ebola Virus Envelope Glycoprotein GP1 Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-containing media were filtered through a 0.45 µm filter (ThermoFisher Scientific) and mixed with fresh media at a 1:1 ratio before adding to pre-seeded target cells ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to virus production medium containing Opti-MEM I (Gibco) + 5 % FBS + 1:100 Glutamax + 1:100 MEM-NEAA (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Nuclease treatment of virus stocks was performed at room temperature by DNase (Ambion), RNase (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Biochemistry 2024Quote: ... cells were given virus production media (DMEM supplemented with 20mM HEPES (Gibco; 15630080) and 30% FBS) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... The sample was subsequently labeled with the TAMRA-alkyne dye by using the Click-It™ TAMRA Glycoprotein Detection Kit (Thermo Fisher Scientific, Waltham, MA). The mixture was vortexed for 5 seconds after the addition of each component according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... these plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
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bioRxiv - Systems Biology 2020Quote: ... and 0.6μg pMD2.G (encoding VSV G envelope protein) was diluted in 2000μl Opti-MEM® Reduced Serum medium (Gibco™/ Life Technologies). 5.4μl PLUS™ Reagent was added directly to the diluted DNA and incubated for 5min at RT ...
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bioRxiv - Cell Biology 2019Quote: ... The RNAi libraries used were a custom library targeting gene products predicted to localize to the nuclear envelope (346 genes, ThermoFisher, lot# AMO20JUZ), and an off the shelf library targeting proteins involved in epigenetic and chromatin regulation (521 genes ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 million HEK 293FT cells were seeded and transfected after 24 hours with the LentiCRISPRV2-IL11g1 or LentiCRISPRV2-IL11g2 construct together with the pLp Δ8,9 packaging plasmid and VSVG envelope plasmid using lipofectamine™ 2000 transfection reagent (Life Technologies, 11668019) and Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Synthetic Biology 2023Quote: ... and envelope plasmids into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s instructions (ThermoFisher Scientific, MA, USA). Cell supernatant containing lentivirus was collected at 24 and 48 hours ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed using TaqMan Fast virus 1 Step PCR Master Mix (Life Technologies), standard curve was drawn with 2019_nCOV_N Positive control (IDT) ...
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bioRxiv - Immunology 2022Quote: ... amplified using the TaqMan Fast Virus 1-Step Master Mix qRT-PCR kit (Invitrogen) on a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... and the virus was produced in HEK293A cells as described by the manufacturer (Invitrogen). Immature brown adipocytes were treated with the viruses on day 2.
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bioRxiv - Microbiology 2019Quote: ... probes and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems, Foster City, CA). We used pre-combined probe and primers (500 nM primers and 250 nM probe ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion-ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... while 4x Taqman Fast Virus 1-Step Master Mix (Life Technologies, Carlsbad, CA, USA) was used for RNA enteric viruses ...
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bioRxiv - Immunology 2021Quote: ... then incubated with the appropriate amount of virus diluted in Fluorobrite DMEM (Life Technologies) medium supplemented with 2% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNA was quantified using qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2021Quote: For Sendai virus iPSC reprogramming using CytoTune™-iPS 2.0 Sendai Reprogramming kit (Invitrogen), fibroblasts were reprogrammed as previously described (Milagre et al. ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus stocks was purified using TRIzol Reagent (ThermoFisher Scientific, catalog no. 15596026). Brief ...
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bioRxiv - Immunology 2022Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region and gene-specific probes and reverse primers as follows:
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix (Cat. n° 4444434, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs were synthesized using random primers and Moloney murine leukemia virus RT (Invitrogen). The RT-PCR products were separated on a 2% w/v agarose-TBE gel ...
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bioRxiv - Neuroscience 2020Quote: ... Fibroblasts were reprogrammed into iPSCs by using the replication-incompetent Sendai virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were quantified by RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and specific primers and probes (Supplementary Table 3) ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolated RNA was reverse transcribed with Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen) and oligo(dT ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from mock or virus infected cells using Trizol reagent (Invitrogen) at 2 and 4 hours post infection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... UKEi001-A was reprogrammed by a Sendai Virus (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). EHT patch casting was performed by mixing CMs and a fibrinogen/thrombin mix3 ...
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bioRxiv - Immunology 2019Quote: ... and inoculated with virus at the optimal MOI of 100:1 in DMEM (Gibco). After 1.5 h ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of virus stocks in Minimum Essential Media MEM medium (Gibco, #41500-018) supplemented with 2% FBS was added to Vero E6 monolayers on 24-well plates (Greiner bio-one ...
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bioRxiv - Zoology 2020Quote: ... 6.25μL of 4X RT-Buffer (TaqMan Fast Virus 1-Step Master Mix, ThermoFisher Scientific), 600nM of the forward primer (RdRp_SARSr-F) ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Eluted RNA was coupled with TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and nCOV_N1 primers/probe (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... virus particles were then purified by gel filtration (7K molecular weight cut-off, Invitrogen).
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bioRxiv - Immunology 2021Quote: ... total RNA was reverse-transcribed with Moloney murine leukemia virus reverse transcriptase (Fisher Scientific) using random hexamers (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... prior to addition of PE243 virus in complete DMEM media +10 mM HEPES (Gibco), at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were conducted with TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems), forward primer in the 5’ leader region ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: UKEi001-A was reprogrammed with a Sendai Virus (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). Expansion and cardiac differentiation of iPSC were performed as recently described1 ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-Influenza A Virus M1 (1:1,000; clone: GA2B; Cat#: MA1-80736; Invitrogen), mouse anti-Influenza A virus M2 (1:1,000 ...