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Citations for Thermo Fisher :
251 - 300 of 711 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... cDNAs for wild-type and mutant M7kd were cloned into the modified pFastBac1 vector (Invitrogen) using the MluI and XhoI sites ...
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bioRxiv - Biophysics 2020Quote: CLGltPh and double cysteine mutants were transformed into Escherichia coli (E. coli) Top10 cells (Invitrogen). Bacterial cells were inoculated in Luria Broth medium containing 100 μg/mL ampicillin at 37 °C until OD600 ~0.6-0.8 ...
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bioRxiv - Microbiology 2019Quote: ... rpvA mutant and the complemented strain were washed twice and resuspended in RPMI1640 (Life Technologies) and the bacteria diluted 1:1000 in 1 mL RPMI1640-10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated mutant Cal09-HA (1.5μg) was complexed with streptavidin conjugated to APC (8μL; Thermofisher #S868) or PE (8μL ...
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bioRxiv - Cell Biology 2022Quote: ... ATF4 mutants were created with the GeneArt™ Site-Directed Mutagenesis System (Cat. A13282, ThermoFisher). Plasmids expressing Myc-tagged Rheb (Plasmid #24941) ...
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bioRxiv - Immunology 2022Quote: ... Wild-type and mutant plasmids were transformed into MAX Efficiency Stbl2 competent cells (ThermoFisher Scientific) for maxi-DNA preparations and each SCIV genome was sequenced to confirm its integrity ...
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bioRxiv - Microbiology 2022Quote: ... The mutant was generated by using the Phusion Site Directed Mutagenesis kit (ThermoFisher, Waltham, MA) with specific mutagenesis primers ...
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bioRxiv - Genetics 2022Quote: ... FSHD2 and control-derived mutant skeletal myoblast cells were grown in high glucose DMEM (Gibco) supplemented with 20% FBS (Omega Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the cysteine mutant Q577C was labeled with AlexaFluor488-C5-maleimide dye (AF488, Thermo Fisher Scientific) as recently described (Sistemich & Herrmann ...
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bioRxiv - Microbiology 2023Quote: IL-33 (residues 112–270) and its R169A mutant were cloned into vector pET28a (Invitrogen) and were expressed with a TEV protease cleavable N-terminal His6 tag ...
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bioRxiv - Developmental Biology 2023Quote: ... Itgb1f/f (mutant) pancreata were dissociated into a single cell suspension using TrypLE (Thermo Fisher). Enzymatic activity was neutralized using FACS buffer (10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: YiiP mutants were labelled with Alexa fluor 488 fluorescent dye (Invitrogen life Technologies, Carlsbad CA) by adding 2.5 μL of dye from a 16 mM stock solution in DMSO to 200 μL of protein at 1-2 mg/ml in SEC buffer supplemented with 10% glycerol at pH 7 ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNAs of 20 wild-type or stat3 mutants was extracted using TRIzol reagent (Invitrogen). cDNA of wild-type and stat3 mutants were generated by the ProtoScript First Strand cDNA Synthesis Kit (NEB ...
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bioRxiv - Genetics 2024Quote: ... Wild-type and mutant clones were then subcloned into pPWM using the Clonase reaction (Invitrogen), resulting in fusion of the coding region to the 6 copies of the myc tag in the 3’ end ...
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bioRxiv - Genetics 2024Quote: 10 µM of purified TREX1 mutant protein and 3✕ SYPRO Orange Protein dye (Life Technologies) were loaded into a 96-well reaction plate ...
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bioRxiv - Neuroscience 2021Quote: ... and fluorescent retrograde tracer cholera toxin B subunit conjugated to fluorophore Alexa 488 (CTB488, Thermo Fisher Scientific, Waltham, MA, USA, C34775) were used to label neurons that projected from the target regions ...
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bioRxiv - Neuroscience 2021Quote: ... A pulled glass needle was introduced in the vitreous through the ora serrate and 2μl of 2μg/μl Alexa Fluor 594-conjugated cholera toxin B (CTB) (Thermo Fisher Scientific, C34777) was injected ...
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Corticocortical Connections of the Rostral Forelimb Area in Rats: A Quantitative Tract-Tracing StudybioRxiv - Neuroscience 2020Quote: ... Cholera toxin beta subunit conjugated to AlexaFluor 647 (CTB647, 5 µg/µL in 0.9% sterile saline, C34778, Invitrogen, Grand Island, NY) was injected (with the same configuration and outside diameter as BDA10kDa ...
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bioRxiv - Neuroscience 2020Quote: ... Small craniotomies were made at these sites and cholera toxin subunit B (CTB) conjugated with Alexa Fluor (CTB-488, −555, −647, Thermo Fisher) was injected using glass micropipettes ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using ketamine / xylazine and intraviteally injected with 2 μl AF488 cholera toxin subunit B (1 mg/ml in PBS) (ThermoFisher Scientific). After 72 h mice were anaesthetized and euthanized via 4% PFA cardiac perfusion ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2 μl of 1.5% cholera toxin subunit B conjugated with Alexa Fluor® 594 (CTB; Thermo Fisher Scientific, Waltham, MA, USA) were injected subcutaneously into the second and third digit of the left and right hind paw ...
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bioRxiv - Neuroscience 2021Quote: ... mice were anesthetized as described before and intravitreal injections of CTB-555 (Cholera toxin subunit B, Alexa Fluor 555-conjugated, ThermoFisher Scientific) were performed ...
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bioRxiv - Neuroscience 2021Quote: ... we injected fluorescently-labeled cholera toxin subunit B (CTB, 500 µg / 100 µL, Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA) into ipsilateral mPFC (Nanofil Syringe and Pump UMP3 ...
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bioRxiv - Neuroscience 2023Quote: ... the retrograde tracer Cholera Toxin Subunit B conjugated with Alexa Fluor 555 or 674 (CTB 555 or CTB 647, 1 mg/ml, Invitrogen, USA) was bilaterally injected with a micropipette connected to Nanoject III microinjector (Drummond Scientific ...
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bioRxiv - Neuroscience 2023Quote: A 10µL Hamilton syringe (QSI Stereotax Injector) containing 0.25 µL of the retrograde tracer Cholera Toxin Subunit B – Alexa Fluor 647 (CTB) (Thermofisher Scientific, #C34778) was fitted with a fine tipped glass capillary tube and lowered into the right adBNST (+0.25 –+ 0.35 mm AP ...
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bioRxiv - Biochemistry 2023Quote: Biotin-conjugated cholera toxin B subunit (CTB) used for the CRISPR screen was purchased from Life Technologies (catalog no. C-34779). Azide-free cholera holotoxin (CT ...
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bioRxiv - Neuroscience 2023Quote: ... Hooke Laboratories adjusted concentrations by lot) followed by the intraperitoneal injection of pertussis toxin (Hooke Laboratories, included in the induction kits) 1x PBS (Gibco, 10010023) on day 0 and day 1 (200-225 ng per animal ...
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bioRxiv - Neuroscience 2023Quote: ... we then injected 0.3 μL of the retrograde tracer cholera toxin subunit B (CTB) conjugated with AlexaFluor-594 and AlexaFluor-488 (Thermo Fisher Scientific) into the msNAc and vsNAc ...
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bioRxiv - Neuroscience 2021Quote: Both wild-type and mutant plasmids were transfected into HEK293T cells using Lipofectamine 3000 reagent (Invitrogen). 24h later ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Molecular Biology 2019Quote: ... respective full length cDNA sequences or mutants were cloned in pENTR™ /D-TOPO® (Invitrogen) entry vectors and transferred to Gateway® destination vectors containing attB site ...
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bioRxiv - Neuroscience 2019Quote: ... or CRMP2 T555A mutant) or 20 pmol of siRNA with 10μl Lipofectamine 2000 (Thermo Fisher Scientific). Twenty-four h after the transfection ...
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bioRxiv - Cell Biology 2021Quote: Plk4 point mutants were generated by PCR-based site-directed mutagenesis using Phusion polymerase (Thermo Fisher) and then cloned into the pMT expression vector ...
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bioRxiv - Microbiology 2021Quote: ... All Vpx mutants were produced by site-directed mutagenesis according to Phusion polymerase manufacture guide (Thermofisher), using the pAS1b-HA-Vpx HIV-2.Gh1 WT (UniP18045 ...
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bioRxiv - Biochemistry 2022Quote: ... Parkin (wt and mutants) was mixed with SYPRO orange protein gel stain (Molecular Probe – Life Technologies) to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid mutant library were purified from the bacteria colonies using PureLink HiPure Plasmid Midiprep Kit (Invitrogen). All primers in this study were ordered from Integrated DNA Technologies.
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 WT and mutant cells were cultured in Minimum Essential Media (MEM, Thermo Fisher Scientific) with 5% fetal bovine serum ...
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bioRxiv - Biochemistry 2020Quote: NPC1−/− cells were transfected with wild type or mutant NPC1 plasmids with Lipofectamine 3000 (Life Technologies) according to the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... hEZHIP and mutant form baculoviruses were produced accordingly to Bac-to-Bac Baculovirus Expression Systems (Invitrogen) after cDNA cloning into pFASTbac vectors ...
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bioRxiv - Genomics 2019Quote: Total RNA was extracted from wild-type and enhancer mutants in duplicate using TRIzol reagent (Thermofisher) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Expression constructs for wild-type and W73A mutant EIF4E were generated by Gateway Cloning (ThermoFisher Scientific) into the pLenti6.3 vector ...
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bioRxiv - Microbiology 2021Quote: The acrA and acrE point mutants were generated using the GeneArt⍰ Gene Synthesis Service (Invitrogen, Germany) and subsequently cloned into the pACYC177 plasmid using the Subcloning Service (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... plasmids encoding for the full-length ySgt2 cysteine mutants were transformed into BL21 Star cells (Invitrogen). Cells were grown in 2x yeast-tryptone (2xYT ...
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bioRxiv - Cell Biology 2021Quote: ... Database search and mutant/WT ratio calculation were performed using Proteome Discoverer 2.4 (Thermo Fisher Scientific) against Sprot Mouse database ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The RAMP2/3 chimeras and the RAMP3 point mutants were ordered as synthetic GeneArt strings (Thermofisher) using the unoptimized human gene sequences including their natural signal sequences and with addition of EcoRI and XhoI restriction sites ...
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bioRxiv - Biophysics 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... the S20C mutant was purified and incubated with excess Alexa FluorTM 488 C5 Maleimide (Invitrogen: A10254) at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Rab7a and LAMP1 wt and mutant molecules tagged with EGFP using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2024Quote: ... The constructs (tβ1AR and L72A mutant isoform) were over expressed in insect cells (sf9, Invitrogen, 11496015) using the Bac-to-Bac baculovirus expression system (Thermo Fisher) ...