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Citations for Thermo Fisher :
501 - 550 of 711 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... S29A/T36A/T45A pro-OCN and S5S/S8A/T15A/S29A/T36A/T45A (i.e., 6XST→6XA) pro-OCN mutant were purchased from Thermo Fisher. Human pre-pro-OCN cDNA cloned into pcDNA3 was purchased from GenScript ...
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bioRxiv - Cancer Biology 2019Quote: ... or mCherry-ER-DD-TRF1-FokI) and the eDHFR constructs (SIM, SIM mutant, or RGG) were transiently expressed by transfection with Lipofectamine 2000 (Invitrogen) 24 hours prior to imaging ...
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bioRxiv - Neuroscience 2020Quote: BHK cells were electroporated with RNA of pSinRep5-HA-GluA1 or the C-terminal mutants and the helper DH (26S) according to Sindbis Expression System manual (Invitrogen) and as previously described (Osten et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... 6X N-terminally Myc-tagged cDNAs encoding Ku70 or Ku80 WT and mutants were cloned into pcDNA/FRT/TO (Invitrogen) and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with pEGFP-human LA (EGFP-wt LA) and mutants after 3h from release of the nocodazole block using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 80-85% confluency and were kept in culture for 24 hrs [67] ...
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bioRxiv - Biochemistry 2020Quote: ... full-length wild-type or mutant-coding sequences (CDS) of AtFN3K were cloned into pENTR/D-TOPO Gateway entry vector (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the genes coding for VyPAL and its mutants were expressed in Sf9 insect cells using the Bac-to-Bac protocol (Invitrogen). Protein purification was performed in three steps with IMAC affinity purification followed by ion-exchange and size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2020Quote: Wild-type PSD-95 PDZ3-SH3-GK (PSG), PDZ3 domain and mutants (all pseudo, engineered F337W in PDZ3 domains) are encoded in modified pRSET vector (Invitrogen) and transformed in Escherihia coli BL21(DE3 ...
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bioRxiv - Microbiology 2020Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific). The wild-type SARS-CoV-2 spike (S ...
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bioRxiv - Microbiology 2021Quote: ... ErbB4 mutant (K751R) or empty control were transfected individually into Vero or U-87 MG cells using Lipofectamine 3000 reagent (Invitrogen) 24 hours prior to drug treatment and infection with rVSV-SARS-CoV-2-S or VEEV-TC-83-nLuc ...
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bioRxiv - Biochemistry 2021Quote: Virus stocks were produced by transfecting HEK293T cells with wild-type or mutant pLAI constructs using the Lipofectamine 2000 Transfection Reagent (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the DNA sequence encoding the Nmono (monomeric N mutant K170A/R185A) was cloned by in vitro recombination (Gateway technology; Invitrogen) from pDONR207 into the yeast two-hybrid vector pPC97-GW for expression in fusion downstream of the GAL4 DNA-binding domain (GAL4-BD) ...
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bioRxiv - Developmental Biology 2020Quote: ... A pMA-T vector encoding for a DNA in which the DSG domain of Arpp19 sequence was exchanged by the cassette domain (D2-D1 mutant) was synthesized by Geneart (Thermofisher) and sub-cloned into Pet-15 6his vector.
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from the culture supernatant of the escape mutant infected cell using TRIzol Reagent (Invitrogen, Carlsbad, CA). RT-PCR was conducted using the Thermoscript RT-PCR system (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Cells in a 6-well plate were transfected with 2 μg of pTM1 P and 1 μg wt or mutant L using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Wild-type and 3D-GNN (replication-defective mutant) RNAs were prepared by diluting 160 ng/well RNA and 0.48 µL/well Lipofectamine 2000 (ThermoFisher Scientific) in opti-MEM™ (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... PHB-DLPCmut*-YFP cDNA was generated by replacing the wildtype START domain with a gene-synthesized mutant variant (GeneArt) using Gibson cloning (ThermoFisher). A Gateway LR reaction then shuttled PHB-DLPCmut- YFP into pMDC7 ...
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bioRxiv - Neuroscience 2022Quote: HEK293 cells and human myoblasts (5×105 cells/well) were transfected with 2ug of AAV.DUX4.V5 (wild type or mutant construct) using Lipofectamine 2000 (Thermo Scientific). Cells were harvested 24 hours post-transfection in TRIzol RNA isolation reagent (Life Technologies) ...
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bioRxiv - Physiology 2023Quote: Myc-tagged wild-type (WT) and PKA-resistant mutant (Ser791Ala) Raptor vectors7 were transfected into CHO-GLP-1R cells using lipofectamine-3000 (Invitrogen). 48h later ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from late (passage 4, P4) CNCCs differentiated from hESC wild-type or mutant cell-lines using Trizol reagent (Invitrogen) for at least two differentiations ...
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bioRxiv - Microbiology 2022Quote: ... or a mutant lacking the nef gene (NL4-3ΔNef) using Lipofectamine 2000 reagent according to the manufacturer’s instructions (Thermo Fisher Scientific). Virus-containing supernatants were collected forty-eight hours post transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and p300BRPHZT 4A- and 4E-substituted mutant proteins were expressed in baculovirus-infected High Five insect cells (Thermo Fisher Scientific). Baculoviruses were produced using the Bac-to-Bac baculovirus expression system (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were transfected with PB2 full-length and mutant overexpression plasmids via a Lipofectamine 3000 Transfection Kit (Thermo Fisher #L3000015). Cells were plated at 300k cells/well in a 6-well plate the day prior to transfection ...
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bioRxiv - Biophysics 2023Quote: ... were transiently co-transfected together with MKK6DD mutants and p38α pcDNA3.1 plasmids (or empty vector for controls) with Lipofectamin2000 (Thermo Scientific) (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products of ame1 and okp1 point mutants (with C-terminal TAF tag and G418 marker) were amplified from the mutant plasmids using a high-fidelity DNA polymerase (SuperFi, Invitrogen) and transformed into the respective haploid ame1 and okp1 null mutants ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequences encoding KNL12026-2342 and ZWINT wild-type and mutants were cloned into a pCDNA5/FRT/TO-EGFP-IRES plasmid and co-transfected with pOG44 (Invitrogen), encoding the Flp recombinase ...
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bioRxiv - Systems Biology 2023Quote: ... NSP16 and NSP10 mutant cDNA entry clones were generated by gene synthesis and subcloning into pDONR221 (GeneArt, Thermo Fisher Scientific). All cDNA clones were sequence verified and shuttled into LuTHy destination plasmids ...
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bioRxiv - Cell Biology 2023Quote: Day 6 moDCs were transfected with GFP-WT cofilin or mCherry-labelled mutant cofilin using a Neon electroporator (Thermofisher Scientific). moDCs were pulsed twice at 1000 V for 40 ms and using 10 µg of the corresponding plasmid ...
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bioRxiv - Plant Biology 2023Quote: ... The WT and mutant NICA PCR products were recombined into the intermediate vector pDonor207 using the BP recombination kit (Invitrogen), and then recombined into the destination vector pEarleygate104 using the LR recombination kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The point mutants of CNNMs were obtained by mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit (Thermo Fisher Scientific). The amino acid sequence of the CBS-pair domain of murine CNNM2 (495-582 ...
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bioRxiv - Biophysics 2023Quote: ... 26 The αSA140C and αSA18C mutants were conjugated with AlexaFluor 647 and AlexaFluor 568 NHS-esters (Thermo Fisher Scientific, USA) targeting the cysteine residues ...
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bioRxiv - Genomics 2022Quote: ... and old (50 days) wDah control and RpII215C4 mutant animals were dissected in replicates of 5 and collected in DMEM (#41965-047, Gibco) without supplements ...
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bioRxiv - Plant Biology 2023Quote: ... genomic DNA from each mutant plant was isolated by using the Phire Tissue Direct PCR Master Mix (Thermo Fisher Scientific), followed by amplifying the sgRNA-targeted regions and subsequent Sanger sequencing of the obtained PCR fragments ...
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bioRxiv - Plant Biology 2023Quote: ... the wild-type and mutant versions were inserted in the pIX-HALO destination vector using the Gateway LR Clonase II Enzyme Mix (Invitrogen). Positive clones were screened by colony PCR and confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... We transfected HEK293T cells with pCAGGS alphavirus E3–E2–(6K/TF)– E1 wild-type or mutant expressor plasmids using Lipofectamine 3000 (Invitrogen) or an empty pCAGGS vector ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were transfected with either wild-type PADI2 (WT) or the PADI2 triple mutant (with L642A/W161A/T159A) using Lipofectamine 3000 (Invitrogen) in 60mm plates ...
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bioRxiv - Cell Biology 2023Quote: ... mRNAs encoding Xenopus SART1 full-length and deletion mutants were prepared from the pCS2+ vectors using mMESSAGE mMachine SP6 kit (Invitrogen). The mRNAs were added to the depleted extract at the beginning of the cell-cycle reactions.
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bioRxiv - Cell Biology 2023Quote: ... and Pikine PfcoroninR100K/E107V-smHA mutant lines (used for all experiments unless specified otherwise) were maintained in complete RPMI 1640 medium (Gibco) supplemented with 10% O+ serum at 4% hematocrit (unless specified otherwise ...
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bioRxiv - Cell Biology 2024Quote: ... or the dominant-negative ATPase-deficient mutant (HSP60-D423A) were previously established using the Flp-In T-REx system (Invitrogen) 56,57 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... or mutant PADI2 (with L642A or T159 or W161A or L642A/W161A, or L642A/W161A/T159A) using Lipofectamine 3000 (Invitrogen) in 96-well plates ...
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bioRxiv - Genetics 2024Quote: ... MDCK cells were transfected with overexpression vectors containing either wild-type or mutant (c.1043C > T) ACTB in Opti-MEM (Gibco) using a NEPA21 electroporator (Nepagene) ...
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bioRxiv - Biochemistry 2024Quote: ... deglycosylated GCase and the GCase N370S mutant were solid-phase coated on the bottom of a 96-well ELISA plates (Maxisorp Nunc-immuno plate ...
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bioRxiv - Cancer Biology 2024Quote: ... The miR-539-5p mimic or NC was cotransfected with the wild-type or mutant plasmid using lipofectamine 3000 (Invitrogen). Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Biophysics 2020Quote: The engineered Cys residue in β or PCNA mutants was covalently modified with tetramethylrhodamine-5-maleimide or Alexa Fluor 546 C5 maleimide (ThermoFisher) and purified from the free fluorophore as described previously (9,10) ...
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bioRxiv - Developmental Biology 2020Quote: HEK293 cells expressing wild-type or mutant ATF6 were lysed in SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (Thermo Scientific). Protein concentrations of the total cell lysates were determined by BCA protein assay (Pierce) ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of xopX and its 14-3-3 protein binding motif mutants were cloned by Gateway cloning (Invitrogen, California) from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP ...
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bioRxiv - Cell Biology 2019Quote: ... Entry clones of myosin XI (WT and mutants) and 3mEGFP were cloned into a moss expression plasmid with LR clonase (Thermo Fisher), yielding pB1-MyoHNC-B5-gtail-mutation-B5-3mEGFP-B2 ...
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bioRxiv - Cell Biology 2019Quote: DNA fragments coding for MKLP1620-858 (wild type and AQA mutant) were generated by PCR and cloned into pDEST15 (Thermo Fisher) to express N-terminal GST tagged polypeptides in E ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... coli cells expressing WT GST-vWA and GST-vWA-C152S mutant proteins were resuspended in PBS supplemented with 2 mM TCEP (Thermo scientific), 0.01 mg/mL lysozyme from chicken egg (SIGMA) ...