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Citations for Thermo Fisher :
451 - 500 of 756 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Full-length yCAF-1 and complex mutants were expressed for 72 hours in High Five cells (Thermo Fisher Scientific) infected with a baculovirus stock of Cac1 with a C-terminal Strep II epitope ...
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bioRxiv - Biophysics 2023Quote: ... and 0.25 μg of wild-type or mutant UL34 plasmid using Lipofectamine as described by the manufacturer (Gibco-BRL) and incubated at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The wildtype and mutant RHCglo plasmids were transfected into 293FT cells using the Lipofectamine 2000 reagent (Cat# 11668019, ThermoFisher) using a suspension transfection method as described previously (PMID ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting plasmid was linearized using XbaI and transformed in the sip mutant by electroporation (Invitrogen NeonTM Transfection system). Transformants were selected on TAP plates containing 20µg/ml hygromycin (Bio Basic) ...
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bioRxiv - Biochemistry 2023Quote: ... GBP1 wild-type and mutants were labeled as described above with AlexaFluor647-C2-maleimide dye (AF647, Thermo Fisher Scientific). Labeling was performed at an equimolar ratio of protein and dye on ice for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant entry vectors were then recombined into pMSCV-FLAG-HA-DEST using LR clonase II (Thermo Fisher, 11791020).
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bioRxiv - Developmental Biology 2022Quote: ... we isolated total protein from 45 dpf adad1Y67X mutant and wild-type testes in RIPA buffer (Thermo Fisher Scientific). After denaturation through heat and 100 mM DTT ...
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 2 μg of plasmid encoding WT S or the indicated mutant using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid mutant libraries were purified from the bacteria colonies using PureLink HiPure Expi Plasmid Purification Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... The complete genome of the ΔdotB mutant and its complemented strainΔdotB::dotB were extracted using the PureLink Genomic DNA kit (Invitrogen) and checked for mutations using two complementary sequencing technologies ...
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bioRxiv - Immunology 2024Quote: ... 250 ng plasmids encoding SARS-CoV-2 spike mutants or ACE2 variants were packaged in Lipofectamine 2000 (Life technologies) and transfected for 24 hours ...
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bioRxiv - Neuroscience 2019Quote: ... 40 Male and 40 female VNCs (20 per sexed replicate) were individually dissected in toxin-supplemented ice-cold calcium- and magnesium-free DPBS (Gibco, 14190–086 + 50 µM D(−)-2-Amino-5-phosphonovaleric acid ...
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bioRxiv - Neuroscience 2024Quote: ... was injected into the soleus muscle and Alexa Fluor 488-conjugated Cholera Toxin subunit B (CTB 488, Thermo Fisher Scientific, 0.05% in 5 µL PBS) into the gastrocnemius muscle ...
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bioRxiv - Plant Biology 2019Quote: ... the coding region of CRK2 or kinase-dead mutants (K353E or D450N) were recombined into pENTR/D-TOPO vector (Invitrogen). pDONRP4P1R/zeo-CRK2pro ...
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bioRxiv - Plant Biology 2019Quote: Real-time fluorescence-based GTP-binding and GTP-hydrolysis assays of native and mutant Gα proteins were performed using BODIPY-GTP FL (Invitrogen) as described previously (Roy Choudhury et al. ...
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bioRxiv - Plant Biology 2019Quote: ... GTPase activity of native and mutant Gα proteins were detected with or without an RGS protein using the ENZchek phosphate assay kit (Invitrogen) as previously reported (Roy Choudhury et al. ...
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bioRxiv - Biophysics 2021Quote: ... Both Fn39-10 S1417C mutant and its inactive version were biotinylated with Maleimide-PEG11-Biotin at residue 1417 (ThermoFisher Scientific) in PBS.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids for expression of mCherry fusion proteins in HeLa cells were constructed by subcloning synthetic genes for Plin4 mutants into pmCherry-N1 (Invitrogen) using BamHI and XhoI restriction sites ...
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bioRxiv - Plant Biology 2022Quote: ... AtSIZ1 and AtSIZ1 mutants were cloned into destination vector pGAD-HA (Dual Systems Biotech) via LR reactions using Gateway technology (Invitrogen). Vector specific primers pDONR207-F ...
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bioRxiv - Physiology 2022Quote: ... human TACAN or their mutants were synthesized by in vitro transcription using the T7 mMESSAGE mMACHINE kit (Invitrogen, Waltham, MA) and injected into oocytes (25 ng each) ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild type and catalytic mutant H672N RNase L plasmids cDNAs (the backbone is pcDNA5-FRT-TO with CMV promoter, Invitrogen) used in this study were a generous gift from Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
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bioRxiv - Molecular Biology 2020Quote: ... and I63S mutants of full-length lamin A/C were inserted into the pcDNA3.1(+) vector (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... the cells with either ATF6 plasmids or ATF6-mutant plasmids were transfected with 200 ng of FGF2-ERSE-luciferase-reporter plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from adult DRG from 6-month old mutant and wild-type mice (4 per genotype, mixed male and female groups) using the RNaqueous Micro kit (Ambion). All DRGs were pooled for each sample to obtain enough RNA for analysis ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... or 1 μg of Super8-FOPflash (8 mutant TCF-binding sites) reporter plasmids (kindly provided by Randall Moon, University of Washington, Seattle) using Lipofectamine 2000 (Invitrogen); a thymidine kinase-Renilla plasmid (0.1 μg ...
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bioRxiv - Developmental Biology 2022Quote: RNA was isolated from heart ventricles of G2-Gata4+/+;Itga4flox/+ control and G2-Gata4Cre/+;Itga4flox/flox mutant E11.5 embryos using the Arcturus Picopure RNA isolation kit (Applied Biosystems), including a step for removing genomic DNA ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cell lines with single integrated copies of KIF4A wild type and the mutants described in the figures were created using the T-Rex doxycycline-inducible Flp-In system (Invitrogen). HeLa cell lines with GFP or mScarlet inserted in frame at the C-terminus of KIF4A and SMC2 in the endogenous loci were constructed using CRISPR/Cas9 (Alfonso-Perez et al. ...
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bioRxiv - Biochemistry 2019Quote: ... the polynucleotides encoding GtACR1 mutants were fused in frame with a C-terminal eight-His tag and subcloned into the pPIC9K vector (Invitrogen) between EcoRI and NotI sites ...
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bioRxiv - Neuroscience 2019Quote: A pool of twenty 5dpf WT and rnaset2sh532 mutant zebrafish larvae were incubated at 27°C for 20 minutes in 5µg/ml of Acridine Orange (Life Technologies) diluted in zebrafish E3 medium ...
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bioRxiv - Microbiology 2019Quote: ... were infected subcutaneously (sc) in each footpad with 103 pfu of nLuc-expressing EEEV mutant viruses in 10 ul of OptiMEM media (Invitrogen). For WEEV ...
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bioRxiv - Microbiology 2021Quote: The parB (WT/mutant) genes were recombined into a Gateway-compatible destination vector pMT571-1xFLAG-DEST via LR recombination reaction (Invitrogen). For LR recombination reactions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 0.2 μg empty pQCXIP or pQCXIP-WT IFITM3 or the indicated mutant fused with amino-terminal FLAG using Lipofectamine2000 (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were co-transfected with either a constant concentration of 0.1 μg of NF1-NanoLuc pcDNA expression plasmid or CRAF RBD-NanoLuc pcDNA expression plasmid with 0.2 μg of GFP-tagged KRAS (WT or Mutant) with 0.25 μl of Lipofectamine 2000 per well following the manufacturer’s protocol (Thermo Fisher Scientific). Twenty-four hours later ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Atg18WT and mutant DNA were amplified from the corresponding pRS316 plasmids and cloned into a pEXP5-NT/TOPO vector (Invitrogen). Plasmids were transformed into E ...
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bioRxiv - Genetics 2021Quote: ... The hGOT1-GFP and mutant hGOT1-GFP constructs were transfected into Chinese Hamster Ovary (CHO) (ExpiCHO-S) cells with an ExpiFectamine Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: RBDwt of SARS-CoV-2 and mutant RBDs (RBDK417N, RBDE484K, RBDN501Y, RBDK417N/E484K/N501Y, and RBDL452R/E484Q) were expressed using Expi293F cells (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and mutant GLP-1R constructs containing designed signal alanine mutation were integrated into CHOFlpIn cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xCENP-C mutants in interphase CENP-C-depleted extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xHJURP truncations or mutants in CSF-arrested or interphase extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Cell Biology 2020Quote: pENTR containing myc-6xHis Sec24C or Sec24D WT or mutants were used in a Gateway LR Clonase II Enzyme reaction (11791020, Invitrogen) according to manufacturer’s instructions with pLenti CMV/TO Puro DEST (670-1 ...
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bioRxiv - Cell Biology 2022Quote: Cells were transfected with the plasmid encoding the DN-KASH or the Rap1Q63E (Rap1*) mutant using Lipofectamin 2000 (Life Technologies).
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bioRxiv - Biochemistry 2022Quote: ... After 3hrs from release of the nocodazole block cells were double transfected with pEGFP-human LA (EGFP-wt LA and mutants) and RFP-HP1α using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 60-70% confluency and were kept in culture for 24 hrs.
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bioRxiv - Plant Biology 2022Quote: ... and mCherry/pDONRP2rP3 into pK7m34GW (phospho-mutants) or pH7m34GW (truncations and DPF deletion) expression vectors (Karimi et al., 2002) by LR Clonase II (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... Resulting mutant variants of CAP1 were cloned in versions with and without stop codons into pDONR221 Gateway entry vectors (Invitrogen) by BP Clonase ...