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Citations for Thermo Fisher :
251 - 300 of 309 citations for CRISPR Screening since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... accumulation of intracellular lipids (Bodipy staining) and cell number (Hoechst staining for nuclei) were quantified with Cell Insight CX5 High-Content Screening (HCS) Platform (Thermo Fisher Scientific) and High-Content Screening (HCS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The AIF and TUNEL translocation to nucleus were further analyzed using the CircAvgInten function of the CellInsight™ CX7 LZR High-Content Screening (HCS) Platform (ThermoFisher, US).
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bioRxiv - Molecular Biology 2021Quote: ... were plated for serological screening and high-throughput nucleic acid extraction using the MagMAX™ mirVANA and CORE pathogen kits (ThermoFisher, Australia).
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bioRxiv - Immunology 2020Quote: ... was added and incubation continued 24 hours before changing the media with Screening media (DMEM+10% FBS containing 10 μg/ml Blasticidin S hydrochloride (Invitrogen, cat#21001)) ...
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bioRxiv - Microbiology 2021Quote: ... plates were kept in dark at 4°C until imaging on a high content screening (HCS) platform (CellInsight CX7 HCS, Thermo Fisher Scientific) with a 10X objective ...
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bioRxiv - Neuroscience 2022Quote: ... The average number of PI-live cells per condition was determined using a CellInsight CX7 High Content Screening Platform (Thermo Fisher Scientific). All conditions were assessed in triplicate.
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bioRxiv - Neuroscience 2022Quote: ... YFP-positive cells from 2-3 mice of each genotype were collected after viability screening with propidium iodide staining (ThermoFisher, Cat. # P3566) on a Beckman Coulter MoFlo platform in the Flow Cytometry and Cell Sorting Facility located in the Ottawa Hospital Sprott Center (Ottawa ...
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bioRxiv - Biophysics 2022Quote: ... Suitable grids and grid regions of hSERINC3-Fab samples were identified by screening atlas images on the UVa MEMC Titan Krios (Thermo Fisher Scientific) using the Falcon 3EC detector ...
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bioRxiv - Biochemistry 2022Quote: For high-throughput screening, DARPins and adapters were expressed in a small-scale format (1 mL culture volume, 96-deep-well plate (Thermo Fisher Scientific)) in the E ...
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bioRxiv - Cancer Biology 2023Quote: siRNA screening was performed in MCF-7 TEAD reporter cells using two different siRNA libraries (Dharmacon On-Target Plus and Ambion Silencer Select) targeting ubiquitin-related genes ...
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bioRxiv - Molecular Biology 2023Quote: ... The grid screening and exploratory dataset acquisition were performed on a Glacios Cryo-TEM equipped with Falcon IV CMOS detector (Thermofisher Scientific (TFS)) with 150 000x magnification ...
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bioRxiv - Bioengineering 2023Quote: ... The EGFP T-REx 293 cell line used for the mammalian screening was obtained modifying the Flp-In™ T-REx™ 293 Cell Line (Invitrogen) for the expression of inducible EGFP according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Clones were selected using white-blue screening on LB agar plates with 100 µg/mL ampicillin and 20 µg/mL X-Gal (Thermo Fisher R0941). Plasmids were extracted using NucleoSpin Mini kit (Macherey Nagel 740588 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 as well as the flanking 5’ and 3’ nuclear localizing sequences and 5’-V5 tag from the GeneArt CRISPR Nuclease Vector (Thermo Fisher Scientific). HEK293-Cas9 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... SVECs lacking the IFN-α/β receptor (IFNAR1-CRISPR) were maintained in the same media as SVECs with 2 μg/mL puromycin (Gibco). Primary LECs (Cell Biologics ...
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bioRxiv - Systems Biology 2021Quote: ... of the cell solution was gently mixed with CRISPR RNP complexes and immediately electroporated according to the manufacturer’s protocols (Neon® Transfection system, Thermo Fisher Scientific) and transferred to a pre-warmed 6-well plates for incubation under standard conditions ...
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bioRxiv - Neuroscience 2020Quote: Genomic DNA from the four experimental clones and a sample of the original Cas9 iPSC line (before CRISPR transfection) were extracted using PureLink Genomic DNA mini Kit (Invitrogen #k1820-02) using included instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 200’000 cells of selected monocolonies of either cell line were seeded in 6-well plates and transfected on next day with CRISPR plasmids using Lipofectamine 2000 (Invitrogen by Life Technologies #11668019) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: Both the constructed all-in-one CRISPR plasmids and dual colored KI plasmids were co-transfected into the mESCs using lipofectamine 3000 (Thermo Fisher Scientific). Dissociated mESCs were plated on gelatin-coated 24-well plates with 500 μl of (t)2iL+Y-27632 medium ((t)2iL+Y) ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: The knockouts were performed using the Alt-R CRISPR-Cas9 system (IDT) and reverse transfection of a Cas9:guideRNA ribonucleoprotein complex using Lipofactamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... a single clone expressing OsTIR1 was amplified and grown in 10 cm dishes before transfecting them with CRISPR/Cas vector plasmids (pLJ869 and pLJ870) and homology donor derived from pLJ851 using Lipofectamine LTX (Invitrogen; Carlsbad, CA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... CRISPR gRNAs were synthesized using a MEGAshortscript T7 Transcription Kit and purified using a mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA). Zebrafish codon-optimized cas9 was synthesized using a mMESSAGE mMACHINE Kit (Ambion ...
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bioRxiv - Molecular Biology 2021Quote: The knockouts were performed using the Alt-R CRISPR-Cas9 system (Integrated DNA Technologies) and reverse transfection of a Cas9:guideRNA ribonucleoprotein complex using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: For the generation of ERAP2 KO EBV-LCLs the Alt-R CRISPR-Cas9 system (Integrated DNA Technologies) was used and cells were electroporated with the Neon Transfection System (Thermo Fisher Scientific). First ...
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bioRxiv - Immunology 2020Quote: ... Caco-2 cells were purchased from DSMZ (ACC-169) and transfected with the Hk2 CRISPR plasmid using Lipofectamin reagent kit (Thermo Fisher Scientific). Positive clones were screened via Western blot to generate a monoclonal population termed Caco-2ΔHk2 ...
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bioRxiv - Cancer Biology 2022Quote: ... were designed using GeneArt (https://www.thermofisher.com/ie/en/home/life-science/genome-editing/geneart-crispr/geneart-crispr-search-and-design-tool.html) and cloned into the GeneArt™ CRISPR Nuclease Vector with OFP (orange fluorescent protein) Reporter (ThermoFisher, Cat# A21174). Twenty-four hours after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... complimentary to Ov-grn-1 exon 1 termed pCas-Ov-grn-1 was constructed using the GeneArt CRISPR Nuclease Vector kit (Thermo Fisher Scientific), as described ...
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bioRxiv - Cancer Biology 2023Quote: ... 5’-GTAGACGCGGTTGCGCTAAG-3’).45 HepG2 cells were transfected with 10 mg of dual-guide RNAs-CRISPR-CAS9-EGFP vector and Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA) and incubated at 37 °C and 5% CO2 for 2 days ...
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bioRxiv - Physiology 2023Quote: ... 100 ng pMD2 and 400 ng of each sgRNA CRISPR Cas9 lentivirus plasmid (plasmid amount rate 3:1:4) using Turbofect transfection reagent (Thermo Fisher Scientific). Lentiviral media was centrifuged once at 1500 x g for 3 minutes and the supernatant was collected ...
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bioRxiv - Cell Biology 2023Quote: ... Vero E6 cells were transfected with the pair of CRISPR plasmids at 40% confluency using the Lipofectamine 3000 system (Thermo Fisher Scientific). Puromycin selection was performed between days 3-6 of culture at 33 μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in a 24-well plate and transfected with CRISPR/Cas and donor plasmids using Lipofectamine 2000 (Thermo Fisher Scientific). Two days post-transfection ...
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bioRxiv - Genomics 2023Quote: Endogenous gene tagging using the CRISPR-Cpf1 system was performed with the electroporation of Cpf1-RNP and DNA donors using the Neon Transfection System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Exon 2 spanning PCR fragments were amplified from gDNA of clonal CRISPR populations and cloned into the pJET1.2 blunt cloning vector using the CloneJET PCR cloning kit (Thermofisher, Waltham MA, USA). The ligation reaction was performed following the manufacturer’s instructions and using 1 μL of PCR product ...
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bioRxiv - Genetics 2023Quote: ... onto chromosome VII using the CRISPR system was synthesized and inserted into the pMX plasmid using Invitrogen GeneArt Gene Synthesis services (Thermo Fisher Scientific). The 6R210(+ ...
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bioRxiv - Cancer Biology 2022Quote: ... HRH1 and CHRM3 CRISPR/Cas9 knockout lentivirus was prepared by transfecting HEK293FT cells with Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, #L3000015) and plasmids including pLP1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cloned lentiviral CRISPR plasmids were verified by Sanger sequencing and co-transfected with LV-MAX packaging plasmids mix (Thermo Fisher, #A43237) into 293T cells using Lipofectamine 3000 transfection reagent (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: We mixed CRISPR-Cas9D10A and sgRNAs for the formation of ribonucleoproteins in OptiMEM reduced serum medium (Thermo Fisher Scientific, Grand Island, NY) at room temperature for at least 1h before electroporation ...
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bioRxiv - Cell Biology 2023Quote: ... HaCaT cells were transfected with a 10 nM duplex of crRNA and Alt-R CRISPR-Cas9 tracrRNA tagged with ATTO 550 (Integrated DNA Technologies) using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... followed by DNA isolation and PCR amplification of CRISPR-targeted loci using Phire Tissue Direct PCR Master Mix kit (ThermoFisher, Waltham, MA). Base editing efficiency was analyzed at the genomic level by Sanger sequencing of the PCR amplicons ...
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bioRxiv - Neuroscience 2020Quote: ... Quantification of immunostaining was performed using the unbiased and semi-automated high-throughput Thermo Scientific Cell-Insight CX5 High Content Screening Platform (Thermo Scientific, Massachusetts, USA). See Supplementary Information ...
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bioRxiv - Cell Biology 2019Quote: ... Ten fields of cells were imaged for each experimental condition with a CellInsight™ CX5 High-Content Screening (HCS) Platform (Thermo Fisher Scientific) using HCS Studio™ software.
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bioRxiv - Molecular Biology 2023Quote: Double-stranded RNAs (dsRNAs) from the DRSC (Drosophila RNAi Screening Center) were generated by in vitro transcription (MEGAscript kit, Thermo Fisher Scientific AMB13345) of PCR templates containing the T7 promoter sequence on both ends as previously described in detail.56 Primer sequences are provided in Table S4.
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bioRxiv - Plant Biology 2024Quote: ... Screening of mutants followed the strategy previously described (35) and included pre-screening of potentially mutated DNA sequences by PCR amplification via DreamTaq (ThermoFisher Scientific Inc., Germany) and high-resolution melting curve analysis (HRM ...
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bioRxiv - Microbiology 2022Quote: A549 and Huh-7 cells were transfected with CRISPR/Cas9 RNP (IDT, Coralville, Iowa) by nucleoporation (NEON Electroporation System, Thermo Fisher, Massachusetts, USA) using the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... J-Lat cells were transfected with CRISPR/Cas9 RNP and tracr-RNA Atto550 (IDT, Coralville, Iowa) by electroporation (NEON Electroporation System, Thermo Fisher, Massachusetts, USA) using the following parameters ...
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bioRxiv - Immunology 2021Quote: ... PCR amplification of the region of gDNA containing the CRISPR editing locus was performed using the SequalPrep Long PCR Kit with dNTPs (Invitrogen, Cat no. A10498) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Wild-type HEK293T and two independent clonal lines of CRISPR Serf2 KO mutant HEK 293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco; high glucose, pyruvate, 41966052), supplemented with 10 % Bovine Cow Serum (BCS ...
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bioRxiv - Biochemistry 2022Quote: ... Affinities of αE2 and αDHED to ERs were measured using the LantaScreen TR-FRET competitive binding assay screening protocol by the SelectScreen Biochemical Nuclear Receptor Profiling Service (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2022Quote: ... 200’000 cells of selected monocolonies of either cell line were seeded in 6-well plates and transfected on next day with CRISPR plasmids using Lipofectamine 2000 (Invitrogen by Life Technologies #11668019) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... driven by the human cytomegalovirus (CMV) immediate early enhancer and promoter was assembled using the GeneArt CRISPR Nuclease Vector system (Thermo Fisher Scientific, Waltham, MA), according to the manufacturer’s protocol ...