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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Live/Dead™ Cell Imaging Kit and CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) were used for detection of cell apoptosis based on the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
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bioRxiv - Neuroscience 2019Quote: ... The washed pellet was either stored at −20 °C overnight or resuspended in 7-12 ml of inclusion body solubilization reagent (Thermo Scientific #78115). The protein suspension was shaken for 30-40 min at 20 °C and then ultracentrifuged at 35,000 x g for 20-30 min at 4 °C.
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Biochemistry 2021Quote: ... The one-step RT-qPCR was run using a qPCR system (QuantStudio 3, Thermo Fisher Scientific, USA) with the following thermocycle ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription and amplification were accomplished in one step in a QuantStudio 3 thermal cycler (Applied Biosystems) using the following incubation program ...
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bioRxiv - Neuroscience 2021Quote: ... The designated samples were then subjected to one-dimensional gel electrophoresis (1DE) employing precast NuPAGE 4-12% Bis-Tris 10-well mini protein gels (Invitrogen, Germany) with 2-[N-morpholino]ethanesulfonic acid (MOPS ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained with 7-amino-actinomycin (7-AAD, ThermoFisher) to label nonviable cells and then sorted using a FACS Aria II sorter (BD BioSciences ...
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bioRxiv - Immunology 2021Quote: ... 7-AAD (Invitrogen), Live/dead fixable viability dye (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatants were loaded on 3–12% NativePAGE Bis-Tris gel (Catalog No. BN1003BOX, Invitrogen, Carlsbad, CA) in NativePAGE running buffer (Catalog No ...
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Pathology 2021Quote: Integra™ Miltex™ 3 mm biopsy punches with plunger system (Fisher Scientific, Cat. # 12-460-407)
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bioRxiv - Systems Biology 2019Quote: ... 3-30μg protein per well was separated using NuPAGE precast 4-12% polyacrylamide gel system (ThermoFisher Scientific). Immunoblotting was carried out using an X-Cell II blot module (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... was added and 10 µL of sample was run on precast 3-12% NativePAGE gels (Invitrogen, BN2011BX10) with NativePAGE anode buffer (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... following the separation of protein complexes on 3–12% precast Bis-Tris Native PAGE gels (Life Technologies), the proteins were transferred to polyvinylidene difluoride (PVDF ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Blue native PAGE (BN-PAGE) was performed using 3-12% precast NativePAGE Bis-Tris gels (#BN1001BOX; Invitrogen). For western blotting ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were run through precast Native PAGE Novex 3-12 % Bis-Tris Protein Gels (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: Native polyacrylamide gel electrophoresis was performed using Invitrogen NativePAGE 3-12% Bis-Tris gels (ThermoFisher Scientific #BN1003BOX) in 1X NativePAGE running buffer (#BN2007) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were separated by Native PAGE™ Novex® 3–12% Bis-Tris Gels (BN1001, Invitrogen™) and transferred to PVDF membrane using an eBlot™ L1 transfer system (GenScript) ...
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bioRxiv - Cell Biology 2023Quote: ... Following separation on a NativePAGE 3-12% Bis Tris protein gel (Thermo Fisher Scientific, cat. no. BN1001BOX), proteins were transferred to a PVDF membrane and incubated with rabbit anti-F1β ...
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bioRxiv - Biochemistry 2023Quote: ... Volumes containing 10 µg of protein were resolved using SDS-PAGE (NuPAGE 3-12% gels, Life Technologies or 10% acrylamide Tris-Glycine ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then loaded onto NativePAGE 3 to 12% Bis-Tris Mini Gels (Thermo Fisher Scientific, #BN1003BOX) along with NativeMark Unstained Protein Standard (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Respiratory chain complexes and supercomplexes were separated on Native PAGE Novex 3%–12% Bis-Tris gels (Invitrogen) for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by NuPAGE 3-8% or 4-12 % Tris-Acetate Midi Gel (Invitrogen, Cat# WG1402BX10) and transferred to nitrocellulose membranes (Fisher ...
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bioRxiv - Genomics 2020Quote: ... The purified RNA samples were quantified using Qubit RNA reagent kit on a Qubit fluorometer 3.0 (concentration range 3-7 ng/μl) (Invitrogen). RNA integrity (RINe ...
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bioRxiv - Genomics 2020Quote: ... media were replaced with 100 μL full melanoma media without phenol-red containing 4μM Caspase-3/7 activity dye (CellEvent, ThermoFisher). Plates where imaged using a Celigo Imaging Cytometer (Nexcelom ...
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bioRxiv - Cell Biology 2019Quote: ... cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol and visualized using FITC 488 nm filter ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... plates were treated with 2 μM of the CellEvent™Caspase-3/7 green detection reagent (Life Technologies, UK) for 60 minutes at 37 °C in the dark ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... seeded with 2,000 cells/well and stained with Hoescht 33258 (1µg/mL) and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at a dilution of 1000x ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Immunology 2020Quote: ... 1×106 CAR T cells were co-cultured with γ-irradiated K562-CD19 or K562-CD19-PD-L1 at 1:3 effector:target (E:T) ratio for 7 days and counted with the countess II Automated Cell Counter (ThermoFisher, USA).
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bioRxiv - Cell Biology 2022Quote: ... 2007) and DsRed2-Mito (Tanaka Bio) plasmids using a ratio of 7:3 using Lipofectamine LTX Plus (ThermoFisher, 15338100) for 5 hours ...
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bioRxiv - Bioengineering 2022Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (16:0 NBD) were purchased from Invitrogen. Phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
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bioRxiv - Biophysics 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-ATTO647N (ATTO-lipid) and (iii) 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-Cyanine 7 (Cy7-lipid) were from Invitrogen, ATTO-TEC and Avanti Polar Lipids ...
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bioRxiv - Cancer Biology 2023Quote: A549 cells grown in 96-well plates were stained with CellEvent Caspase 3/7 green detection reagent (Thermo Scientific) according to manufacturer’s instructions and the fluorescence measured using a plate reader at 488 nm wavelength ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was monitored every 24 or 72 hr for 3-7 consecutive days using CyQuant Reagent (Invitrogen, C35011) by measuring bottom-read fluorescence at 520 nm with excitation wavelength set at 480 nm using EnSight Multimode Plate Reader (PerkinElmer) ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 3 × 106 HEK293F cells were transfected with one of these constructs using 293fectin Transfection Reagent (Thermo Fisher) or 293-Free (EMD Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... This infection mixture was added to 1.5 x 106 Jurkat cells and incubated in one well of a 12 well tissue culture plate (Fisher Scientific, Cat. 150628) at 37 °C with 5% CO2 for 5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... one entire series of the hippocampus (every 9th section) was slide-mounted onto charged slides (Fisher Scientific, 12-550-15, Pittsburgh, PA). Slide-mounted sections underwent antigen retrieval (0.01 M citric acid pH 6.9 ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Immunology 2020Quote: ... from each sample was reverse-transcribed using the qScript™ One-Step SYBR® Green qRT-PCR kit in a ViiA-7 RT-PCR system (Thermo Fisher Scientific). The GAPDH gene was used as the endogenous control ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Physiology 2023Quote: ... For cell viability assays 7-AAD (7-Aminoactinomycin D, Invitrogen, A1310) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was quantified using a Nanodrop One-One (Thermofisher) before cDNA amplification or RNA seq libaries preparation.
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bioRxiv - Immunology 2021Quote: ... Calu-3 cells were maintained in Dulbecco’s Modified Eagle Medium supplemented with Ham’s F-12 (Thermo Fisher Scientific), 2% Fetal Bovine Serum and 1% Penicillin/Streptomycin in a humidified 37°C incubator (5% CO2 ...