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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The concentration was measured at NanoDrop One/One (Thermo Fisher Scientific) and the quality (RNA integrity number ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Dulbecco’s modified Eagle’s medium F-12 (DMEM/F-12; Gibco), MACS NeuroBrew-21 (130-097-263 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell extracts (20 µg/lane) were fractionated in 3%-8% or 4-12 % tris-acetate gel (NP0006, NuPAGE Life Technologies). All antibodies were used at the concentrations recommended by the manufacturers.
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bioRxiv - Cell Biology 2020Quote: ... IP and lysate samples were separated by SDS-PAGE on precast 3-8% tris-acetate and 4-12% bis-tris acrylamide gels (Invitrogen) followed by transfer to 0.2 μm nitrocellulose membranes (Amersham) ...
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bioRxiv - Genetics 2020Quote: ... Samples were loaded on 4-12% NuPAGE Bis-Tris gels or 3-8% NuPAGE Tris acetate gels (Thermo Fisher Scientific) and transferred to the nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ul of each sample and 3% input of each cell lysate was loaded on a 4-12% Bis-Tris pre-cast SDS-page gel (Invitrogen) and transferred to a PVDF membrane (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid pCR8-pilA1-ORF was recombined with plasmid pAX1-GW(12) (Table 3) using the Gateway LR recombination protocol (Invitrogen). The resulting plasmid ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from Hela cells (n = 3) subjected to EBSS-induced autophagy and treatment with 5 μM UNC0638 for 12 hours using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Cell lysates dissolved in SDS load buffer were heated at 90°C for 3 min and separated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to a PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Media was changed every 12 hours for the first 3 days with fresh hESC medium with basic fibroblast growth factor (bFGF) (Invitrogen) and ROCK inhibitor (Y-27632 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected with 3 μg pGuide-it-tdTomato with inserted TLR5-specific guides and 12 μl Lipofectamine 2000 transfection reagent (Invitrogen) per well ...
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bioRxiv - Cell Biology 2021Quote: Extracts were analyzed by SDS–PAGE using a 4-12% Bis–Tris or 3-8% Tris-Acetate NuPAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: A total of 50 μg of protein for each of the samples was loaded into a pre-cast NativePAGE 3-12% Bis-Tris gel (Invitrogen). For the run ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins were briefly subjected to SDS-PAGE (3 min at 200 V, 4– 12% Bis-Tris gel, Life Technologies) and stained with InstantBlue Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... so that 50 μg of total protein could be mixed with 5% Coomassie brilliant blue G-250 before loading on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hr ...
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Microbiology 2022Quote: A total of 1.5 µg/well of VA1 IVT RNA was transfected into BHK-21 cells in 12-well plates using 3 µL/well of Lipofectamine MessengerMax (Invitrogen) and following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 40 whole embryos at stage 3 (18 h AEL) were homogenized using disposable pellet pestles (12-141-368; Fisher Scientific) and 1.5 mL Eppendorf tubes (0030125150 ...
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bioRxiv - Cell Biology 2022Quote: ... and 12 μl of PLUS reagent were added to 3 ml of Opti-MEM Reduced Serum Media (ThermoFisher 31985-062) and incubated for 5 minutes ...
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bioRxiv - Plant Biology 2019Quote: ... 50 mg mL−1 Serva Blue G) and applied to the precast native 3-12% Bis-Tris polyacrylamide gel (Invitrogen, Life Technologies Corporation ...
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bioRxiv - Microbiology 2020Quote: ... 100 ng of extracted vRNAs were reverse transcribed using a Uni-12 primer (5’-AGCRAAAGCAGG-3’) and Superscript III reverse transcriptase (Invitrogen). Quantification was performed by qPCR on a Rotor-Gene Q 2plex System (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were briefly subjected to SDS-PAGE (3 minutes at 200 V, 4-12% Bis-Tris gel, Life Technologies), and stained with InstantBlueTM Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 2500 Vero-E6 (12 μl/well) or 10000 Calu-3 (12 μl/well) were seeded in 384-well white optical-bottom tissue culture plates (Nunc) with the Multidrop Combi liquid handling instrument (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The supernatants containing both the cytosolic and glycosomal proteins were analysed by BN-PAGE on a precast (3–12%) Bis-Tris polyacrylamide gel (Invitrogen) according to standard methods.
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bioRxiv - Developmental Biology 2021Quote: ... Purified OPCs were then seeded onto poly-D-lysine-coated 12-multiwell plates at a density of 3 × 104/cm2 in OPC proliferation medium composed of Neurobasal medium (Gibco), 2% B21 (Miltenyi biotec) ...
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bioRxiv - Cell Biology 2019Quote: ... 40 μg were loaded in NativePAGE Novex Bis Tris Gels (3-12 %) and transferred on a PVDF membrane according to the manufacturer’s instructions (ThermoFisher Scientific). Gels were electrotransferred to Hybond-P nitrocellulose membrane (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... or 1×104 Calu-3 (12 μl/well) were seeded in 384-well white optical-bottom tissue culture-treated plates (Nunc) with a Multidrop Combi liquid handling instrument (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Cell Biology 2019Quote: ... 15 ul of the first elution (3% of total) was loaded on NuPAGE 4%–12% Bis-Tris gels (Life Technologies) and analyzed by silver staining.
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bioRxiv - Cell Biology 2019Quote: ... These prepared samples were loaded on 3–12% Bis-Tris Native PAGE gels and electrophoresed using 1×Native PAGE Running buffer system (Invitrogen). The cathode buffer included 1×Cathode Buffer Additive (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen) running buffer ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... the samples were mixed with Coomassie G-250 and resolved in 3-12% NativePAGE Bis-Tris gel (Invitrogen Catalog #BN1003BOX) as per the manufacturer’s recommendations ...
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bioRxiv - Genomics 2022Quote: ... and measuring the fraction of sgRNA-expressing cells 3-12 days after transduction as mCherry-positive cells by flow cytometry on an Attune NxT (ThermoFisher). All experiments were performed in duplicates from the infection step.
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bioRxiv - Plant Biology 2022Quote: ... An equivalent to 100 mg of mitochondrial proteins were loaded and separated on 3–12% (w/v) polyacrylamide NativePAGE Bis/Tris gels (Invitrogen). Following electrophoresis ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were lysed with 5 cycles of 30 s 6500 ms-1 / 30 s on ice in an MP Fastprep bead beater or for 3 min at power 12 in a Bullet Blender (ThermoFisher) in cold room ...
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bioRxiv - Cell Biology 2022Quote: ... These prepared samples were loaded onto 3–12% Bis-Tris Native PAGE gels and electrophoresed using a 1× native PAGE running buffer system (Invitrogen). The cathode buffer included 1× cathode buffer additive (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5% Coomassie brilliant blue G-250 was added before loading ~100 μg on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hours ...
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bioRxiv - Microbiology 2022Quote: ... isolated primary cells were co-cultivated with gamma-irradiated mitotically inactivated NIH3T3 mouse embryonic fibroblasts (MEFs) in a 3:1 mixture of Ham’s F-12 nutrient mix (Life technologies) and DMEM supplemented with 5 % FCS ...
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bioRxiv - Plant Biology 2023Quote: ... samples were loaded and run on Native PAGE 3%-12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were separated on a NativePAGE Novex 3%–12% Bis-Tris protein gel system (Thermo Fisher Scientific, catalog BN1003BOX) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed with 5 cycles of 30 s 6500 ms-1 / 30 s ice in an MP Fastprep bead beater or for 3 min at power 12 in a Bullet Blender (ThermoFisher) in cold room ...
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bioRxiv - Molecular Biology 2023Quote: ... 5×106 cell equivalents were loaded into wells on a 3-12% Bis-Tris gradient gel with unstained NativePage protein standards in the first well (ThermoFisher) and run according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...