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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 7 Oxa 12 thia spiro 5.6 dodecan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: The number of epithelial cells in the Cervix Chip was obtained by digesting the Cervix Chip apical channel at day 7 of differentiation or day 3 on co-culture with bacteria with Collagenase IV (Gibco, Cat. No. 17104019) in TrypLE (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2019Quote: ... 1.0 x105 cells were seeded in 12-well plates (n=3/condition/cell-type) and cultured in monolayer in differentiation media (StemPro Osteogenic Media; ThermoFisher Scientific) for 28 days ...
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bioRxiv - Biophysics 2019Quote: ... pieces of tissue containing 2-3 tracheal segments were dissected in ice-cold Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (ThermoFisher, DMEM/F12). Tissue sections were sliced open along the proximal-distal axis from the dorsal side and mounted onto 35mm cell imaging dishes (Mat-Tek) ...
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bioRxiv - Biophysics 2021Quote: ... between 0.5-2 mg of individual histones were combined and dialyzed into 2 M NaCl (10 K MWCO, 3-12 mL Slide-A-Lyzer Dialysis Cassettes, ThermoFisher Scientific). After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... cells were mounted on pre-cleaned frosted glass cover slides (Fisher 12-552-3) with ProLong Diamond antifade reagent with DAPI (Invitrogen P36962). After curing for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Immunology 2022Quote: ... Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... was added to the supernatant and 100 μg of protein was loaded onto a 3-12% native gel (NativePAGE™ (Invitrogen)) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on a commercial native- PAGE gel (3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well, Invitrogen, Cat. BN1001BOX) at 100 V tension using as running buffer 50 mM BisTris ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: ... First dimensional BN-PAGE was run on NativePAGE™ 3-12% Bis-Tris gels under native conditions at 150V for 30 minutes with 1x native cathode buffer (Invitrogen) followed by 90 minutes at 250V in 0.1x native cathode buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Prepared samples were run on NativePAGE™ 3-12% Bis-Tris gels under native conditions at 150V for 30 minutes with 1x native cathode buffer (Invitrogen) followed by 90 minutes at 250V in 0.1x native cathode buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were centrifuged at full speed for 3 min before loading on NuPage 4%–12% Bis-Tris Gels (Invitrogen, Carlsbad, CA). Gels were run at 140V for 55 min ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Neuroscience 2022Quote: ... on laminin-coated plates (3 μg/mL) for two passages on day 12 and 16 (FPp1 and FPp2 respectively) with Accutase (Invitrogen™) to a 1:4-6 split ratio onto laminin-coated plates with 5 μM of RI ...
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bioRxiv - Biophysics 2022Quote: ... The purified samples were also incubated in seven different buffers ranging in pH from 3.0 to 12.0 and then subjected to Blue Native PAGE using a NativePAGE™ Sample Prep Kit and a 3–12% Bis-Tris Gel (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... The HEK293T cells were seeded in 96-well plate in quadruplicate and co-transfected with each of 12 microRNAs and the wild-type FOXP2 3’UTR firefly luciferase reporter construct using Lipofectamine 2000 (Life Technologies) for 26 hours ...
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bioRxiv - Physiology 2022Quote: ... The samples and standards were then loaded onto a native PAGE 3 to 12% Bis-Tris Gel (BN1001BOX, Thermo Fisher Scientific), and electrophoresis was performed at 150 V for 3 hours on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Physiology 2022Quote: Primary neuronal cultures 12-14 days post isolation were incubated for 30 min with Fluo-4AM (Thermofisher, 3 μM final concentration) according to the manufactures instructions in artificial cerebral spinal fluid (ACSF) ...
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bioRxiv - Physiology 2023Quote: CHO-Kv7.2/7.3 and HEK-CaSR were cultured separately in Gibco™ Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 (Life Technologies) supplemented with 10% foetal bovine serum (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The INT-based rate is converted to O2 consumption using 3 unique 12-point dilution-based standard curves of freshly prepared Fomazan dye (Fisher Scientific). To compare the O2 drawdown method to the INT methods ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant was mixed with 0.25% G-250 Sample Additive and loaded on a NativePAGE 3-12% Bis-Tris gel (BN1001BOX, Invitrogen, CA, USA) for electrophoresis.
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... to a final detergent:dye ratio of 16 g/g before running on a 3-12% NativePAGE Mini Protein Gel (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected into AtT-20 cells (2.0× 105 cells/well) using 12-well plates and 3 μL of the Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) by reverse transfection ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting pellet was resuspended in DMEM/F-12 and cell density was determined using the Countess 3 Automated Cell Counter (Invitrogen, AMQAF2000) using Trypan blue solution (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells from 2-D or 3-D cultures were harvested from one well of a 6-well plate and dissociated with Accutase (Thermo Fisher #A1110501), washed three times with 1x PBS ...
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bioRxiv - Genomics 2022Quote: ... HEK293FT cells were replated at a 1:3 dilution one day post-transfection into media supplemented with 1 μg/mL final concentration puromycin (Thermo Fisher Scientific). HEPG2 cells (American Type Culture Collection (ATCC – HB8065 ...
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bioRxiv - Cell Biology 2021Quote: ... 1ml of bone marrow was incubated in one well of 6-well plate with 3 ml StemPro™ MSC serum-free medium (Gibco, Thermo Scientific). Media was changed after every two days until it reached to confluency ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using OC43-nucleocapsid specific TaqMan primers and a probe 18 fluorescent- labelled with a 5’-FAM reporter dye and 3’-BHQ quencher (IDT) and AgPath-ID™ One-Step RT-PCR kit (AgPath AM1005, Applied Biosystems) on an ABI QuantStudio 3 platform (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... HEK293FT cells were replated at a 1:3 dilution one day post-transfection into media supplemented with 1 µg/mL final concentration puromycin (Thermo Fisher Scientific). HEPG2 cells (American Type Culture Collection (ATCC – HB8065 ...
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bioRxiv - Synthetic Biology 2022Quote: ... situated between nsP4 and the viral 3’ untranslated region (UTR) were reverse-transcribed and PCR-amplified using the SuperScript™ IV One-Step RT-PCR System (ThermoFisher, #12594025) and the 26S-F primer and pooled SinRev primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...
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bioRxiv - Genetics 2020Quote: ... RNA was quantified using a NanoDrop™ One (#ND-ONE-W, ThermoFisher Scientific) and 2 μg per sample were used for cDNA synthesis by performing a retro-transcription reaction with the SuperScript™ VILO™ cDNA Synthesis Kit (#11754050 ...
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bioRxiv - Microbiology 2021Quote: ... as measured by NanoDrop One/One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific) was loaded onto a 50-cm μPAC capLC column (PharmaFluidics ...
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bioRxiv - Microbiology 2022Quote: NanoDrop One Microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific, catalog #: ND-ONE-W).
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bioRxiv - Cancer Biology 2021Quote: ... RNA purity was assessed using a NanoDrop One (ThermoFisher Scientific, ND-ONE-W). The RNA samples (1μg ...
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bioRxiv - Microbiology 2022Quote: ... One infected and one uninfected flask were treated with DSS Crosslinker (Thermo Scientific) following manufacturer instructions for intracellular crosslinking ...
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bioRxiv - Biophysics 2022Quote: ... RNA was then quantified using a Nano Drop One (Thermofisher #ND-ONE-W) and the concentration adjusted to 60 ng/uL ...
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bioRxiv - Immunology 2023Quote: ... the IgG concentration was determined using NanoDrop One (Thermo Fisher, ND-ONE-W). All monoclonal antibodies were assessed for integrity by SDS-PAGE analysis.
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bioRxiv - Physiology 2023Quote: ... One hundred microliters of One-step Ultra TMB ELISA substrate (Thermo Scientific #34028) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined using a NanoDrop One (Thermo Fisher, ND-ONE-W) using absorbance.
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded using 7-AAD (7-Aminoactinomycin D, Thermo Fisher Scientific, 00-6993-50). Cells were analyzed using a FACSCanto II flow cytometer (BD ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated on 4-12% or 12% Bis-Tris gels (Invitrogen), transferred to nitrocellulose membranes ...