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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... commercially available CRISPR control targeting HPRT (IDT) and KANK1 exon-targeting (ThermoFisher Scientific, 5’-GUCUAGUUGAUAACCAUAGG-3’) gRNAs were also obtained ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) (Thermo Scientific) and 1 mM isopropyl beta-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 x 5 minutes in PBST and then incubated with secondary antibodies (Invitrogen) diluted in PBST + 0.2% BSA for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: A 3 or 5 wt% solution of GelMAL was prepared by dissolving GelMAL in PBS (Gibco Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with siRNA (5’-GGACTGAGGUUGUCAAGAA-3’) for PRC1 using Oligofectamine (Life Technologies, Carlsbad, CA) as previously described (Udy et al. ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Microbiology 2022Quote: ... Amplifications were achieved using a 3-step program on a QuantStudio 5 system (Thermo Fisher Scientific). Transcript abundance was calculated using the ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was carried out on a QuantStudio 3 or QuantStudio 5 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged approximately every 3-5 days (70-85% confluency) using Accutase (Gibco, #11105-01) to detach cells ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Physiology 2023Quote: ... R: 5’ TGCCTTCGTGCCGTACTG 3’) and confirmed by DNA sequencing following sub-cloning into pCRTM-Blunt (Invitrogen). Further genotypes were determined by real-time PCR with specific probes designed for each gene (Transnetyx ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Physiology 2023Quote: ... were incubated for 20 minutes with 5 µM of Di-3-ANEPPDHQ (Thermo Fisher Scientific, USA) membrane dye ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid containing a sgRNA targeting APC (5‘-GGCACTCAAAACGCTTTTGA-3‘) using lipofectamin-2000 (Thermo Fisher Scientific, 11668027). WT organoids were grown in WENR medium and APC truncated organoids were grown in ENR medium and are later refererred to as mCol WT and mCol APC ...
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bioRxiv - Molecular Biology 2022Quote: ... pDNOR207-ZNF451-1 (isoform 1) and pDNOR207-ZNF451-3 (isoform 3) were generated using the Gateway® cloning BP reaction (Thermo Fisher Scientific) upon cDNA amplification using BP-tailed primers and pDNOR207 as donor vector ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.
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bioRxiv - Cell Biology 2022Quote: ... 3 ml was diluted 1:1 with PBS (Thermo Fisher Scientific, Waltham, MA) and kept on ice for purity analysis by flow cytometry ...
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bioRxiv - Neuroscience 2020Quote: ... Capture proteins were eluted by heating (98°C) the beads in 100 μl of SDS-PAGE sample buffer (ThermoFisher) for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... a 77 amino acid length peptide with >98% purity as determined by analytical HPLC was synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2022Quote: ... were further confirmed by matching the retention times and mass spectra with authentic analogues (all purity >98%; ACROS Organics). For a particular compound ...
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bioRxiv - Cell Biology 2024Quote: ... a premium microscope slide (Fisher-finest, 3″ × 1″ × 1 mm; Thermo Fisher Scientific, 12-544-1) or chambered coverglass (1 well ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and 50 µL of 50 mg/mL 1-ethyl-3-[3-dimethyl-aminopropyl]-carbodiimidehydrochloride (Thermo Fisher Scientific, 22981) were simultaneously added to the reaction tubes ...
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bioRxiv - Biochemistry 2020Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Immunology 2023Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2024Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Bioengineering 2019Quote: ... native gels were run with 5 μL per well of a 1:5:1 mixture of sample:water:BlueJuice loading dye (Thermo Fisher Scientific) in a 10% TBE ...
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bioRxiv - Microbiology 2022Quote: ... The infected cells were resuspended in HL5c containing 5 U mL−1 of penicillin and 5 μg mL−1 of streptomycin (Invitrogen) to prevent extracellular bacterial growth ...
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bioRxiv - Biophysics 2021Quote: ... 1 µM of TO-PRO-3 iodide (Thermo Fisher, T3605) was added for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... embryos were incubated in ToPro-3 (1/1000, Molecular Probes) for 1h as previously described (Roussigné et al. ...
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bioRxiv - Microbiology 2019Quote: ... or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000; Ambion), or anti-p24 (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-CENP-A (Clone 3-19, Invitrogen; 1:1000 dilution) and anti-“Bonsai”/NDC80 (68 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Lamin B2 (mouse, Invitrogen, clone E-3, 1:500), anti-EEA1 (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg mL−1 Cascade Blue (3 kDa, Thermo Fisher) was added to the antibiotic-free expansion media in the top epithelial channel with a flow rate of 30 μL hr−1 ...
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bioRxiv - Immunology 2022Quote: ... cDNA was diluted 1:3 in nuclease-free water (Ambion) and added to 5 μL SyGreen HiROX mix (PCR Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-Galectin-3 (1:200, Invitrogen, 14-5301-82); rabbit anti-Fibronectin (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... or Toto-3 iodide (T3604, Invitrogen, MA, USA; 1:500) diluted in PBST ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were counterstained with TO-PRO-3 (1:5,000, Invitrogen) together with secondary antibodies when necessary ...
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bioRxiv - Developmental Biology 2019Quote: agar (3 gr/l, Fisher Scientific UK Ltd, BP2641-1), malt (64.3 gr/l) ...
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bioRxiv - Microbiology 2020Quote: 1 μL of random primers at 3 μg/μL (Invitrogen) were added to fragmented RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... nuclei were stained with 1 µg/mL TOTO-3 (Invitrogen) in PBS for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Transfection was performed using 1:3 DNA: Lipofectamine 2000 (Invitrogen) ratio ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-INSR (1:50, clone CT-3, Invitrogen, AHR0271).
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bioRxiv - Immunology 2021Quote: ... either with 1 μM FluoZin-3-AM (Thermo Fisher scientific) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... with a 1:3 ratio of siRNA:RNAiMax (Thermo Fisher Scientific). 48 hours post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... All lines were maintained in 3:1 DMEM (Gibco, 11960044): F12 media (Gibco ...
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bioRxiv - Bioengineering 2022Quote: 1” x 3” glass microscope slides (12-550-A3, ThermoFisher) were cleaned with 0.1% Triton X-100 (T9284 ...