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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 μl of dithiothreitol (DTT, 5 mM; Invitrogen), 1 μl of RNase-OUT (40 U ...
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bioRxiv - Genomics 2021Quote: ... 5 μL Streptavidin C-1 beads (Thermo Fisher) were Washed with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 mL acid phenol: chloroform 5: 1 (Ambion), and 50 mL 0.5 mm zirconia/silica beads (BioSpec) ...
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bioRxiv - Neuroscience 2023Quote: ... anti-claudin-5 (Invitrogen, USA, 1:1000 dilution), anti-p-ERK1/2 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... β-Tubulin (1:5 000, MA5-16308, Thermo Fisher) PINK1 (1:1 000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in 1xPBS/0.05% Tween for 3 rounds of 5 minutes and incubated in DAPI (Invitrogen Molecular Probes D1306: 1:10,000 from 5mg/mL stock) for 10 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... for 10 min at 98°C and were loaded onto pre-cast NuPAGE 4-12% (w/v) Bis-Tris 1.5-mm minigel (Invitrogen™). The electrophoresis was carried out at 180 V in NuPAGE MES SDS running buffer (50 mM Tris base ...
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bioRxiv - Synthetic Biology 2019Quote: ... The samples were incubated at 98°C for 10 minutes and 10 μl of each mixture and a SeeBlue Plus2 pre-stained protein ladder (Thermofisher) were loaded onto a NuPAGE 4-12 % Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... and 2mM PMSF) and heated to 98°C for 30 min and 90°C for 2h on a heat block (ThermoFisher). Samples were mixed well and centrifuged at 22,000 × g for 30 min at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 20mg of dorsal muscle tissue was dissected and immediately submerged in 500μl of homogenization buffer (98% RIPA Lysis Extraction Buffer (ThermoFisher #89900), 1% Halt Protease/Phosphatase Inhibitor Cocktail (Thermo Scientific #78442) ...
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bioRxiv - Genetics 2019Quote: ... in 0.1 M sodium bicarbonate that was incubated with 98 μg of Alexa Fluor 647 succinimidyl ester (A20006, Thermofisher Scientific) for 1h at room temperature (3:1 dye:protein mole ratio) ...
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bioRxiv - Immunology 2023Quote: ... and STX-S_pLenti at a ratio of 5:5:1 using Lipofectamine 3000 (ThermoFisher, L3000008) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: ... and 2-3 μL of the freshly phase-separated sample was placed into a chamber made on a glass slide (Fisher Scientific 3” × 1” × 1 mm). The chamber made by using double-sided tape was then sealed with a square coverslip to avoid evaporation of the sample ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with following primary antibodies and/or labelling agents: anti-GFP (Invitrogen, #11122, 1:800, 3 days or Invitrogen, #10262, 1:800, 3 days), anti-V5 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: Caspase-3-like activity was quantified using the EnzChek Caspase-3 Assay kit #1 (Molecular Probes, Eugene, OR, USA). Tissue samples were thawed on ice for 5 min ...
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bioRxiv - Biophysics 2020Quote: ... hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine) (Invitrogen). Cleavage of PED-6 eliminates a self-quenching effect that results in the release of a BODIPY-FL dye ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
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bioRxiv - Genetics 2019Quote: ... resuspended in 60 ml hypotonic lysis buffer (20 mM HEPES-KOH pH 7.5, 5 mM NaCl, 1 mM MgCl2, 1 mM PMSF and EDTA free protease inhibitor mixture from Roche and Thermo Scientific) and incubated for 15 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... (anti-Claudin-1, anti-Claudin-5, anti-Occludin, anti-ZO-1; Invitrogen), in a 1:100 dilution in 10% donkey serum in PBS with 0.1% Saponin and incubated overnight on the Brain-Chip at 4°C ...
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bioRxiv - Genetics 2019Quote: ... and 5 µl of protein A: protein G 1:1 Dynabeads (Thermofisher). Antibody-bond chromatin was washed four times in low salt buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cludin-5 (1:1000) or -γ-tubulin (1:3000, Thermo Fisher Scientific) in Tris-buffered saline (Tris 50 mM and NaCl 150 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μL of T4 DNA ligase (5 U μL−1, ThermoFisher Scientific) and 2 μL of Milli-Q H2O (adjusted as needed to reach the total volume) ...
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bioRxiv - Plant Biology 2019Quote: ... the full-length GAD2 was also amplified using primer set GAD2_forward2 (5’-CACCATGGTTTTGACAAAAACCGC-3’) and GAD2_reverse and cloned into pENTR/D-TOPO vector via directional cloning (Invitrogen), followed by a LR reaction recombinant into pMDC3240 ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed on QuantStudio™ 3 and 5 Real-Time PCR System (Thermo Fisher Scientific, USA) using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
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bioRxiv - Cancer Biology 2022Quote: ... for 5 minutes followed by a wash with chilled PBS and blocked with 3% goat serum (Thermo Scientific) for 1h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 3-5 μl of purified γ-TuRC was visualized on an SDS-PAGE with SYPRO Ruby stain (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... hBMSCs (passage 3) were expanded at 37°C and 5% CO2 in expansion medium (Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... by incubating muscles twice for 45 min in preoxygenated Ringer containing 5 μM Rhod-3 AM (Life Technologies), 0.02 % Pluronic acid (Life Technologies ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Neuroscience 2020Quote: ... brain sections from 3- to 5-month-old mice were exposed to 2.5 µM MitoSOX Red (Molecular Probes), at 37°C for 30 min14 ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was sonicated for 3-5 mins using an ultrasonic cleaner (FS220, Thermo Fisher Scientific, Waltham, MA). The solution was diluted to 10 mL with 2% nitric acid ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 20 ng of RNA were amplified and labelled with the GeneChip 3’ IVT Pico Kit (Affymetrix) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 3 to 5 min in 100 μM Lysotracker-Red DND-99 (Life Technologies, Carlsbad, CA, USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc ...
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bioRxiv - Genomics 2020Quote: ... slides were washed 3 times for 5 min with TNT and mounted with Prolong Gold (P36930, Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... miR-125b+A or control RNA 3’O-methyl-modified mimic using 5 μl Lipofectamine 2000 (Life Technologies) in a 500 μl reaction mixture according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... rinsed again (3 × 5 min) in 1X PBS and mounted using SlowFade™ Diamond Antifade Mountant (Invitrogen; S36963). Fluorescence images were acquired with a confocal laser scanning microscope (LSM 700 or 710 ...
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bioRxiv - Microbiology 2019Quote: ... PUUV-Gc-rev 5’-ATTCCCGGGCTTGTGCTCCTTC-3’ to PCR amplify (Phusion Flash High-Fidelity PCR Master Mix, ThermoFisher Scientific) the inserts from codon optimized PUUV GPC described in Iheozor-Ejiofor et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The bands were detected by using SuperSignal pico/femto chemiluminescence kits (Thermo Scientific; #’s1859022/3 and 1859674/5).
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 and medium were changed every 3 days and cells were passaged with 0,05% trypsin-EDTA (Invitrogen) twice per week.
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bioRxiv - Genomics 2020Quote: ... Biotin labelled fragments which contained the 5’ and 3’ connecting region was enriched using Dynabeads M280 streptavidin (Invitrogen) and incubating at room temperature for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were washed 3 times 5 minutes in PBS and mounted using ProLong Gold mouting media (Thermo Fisher) under glass cover slips.
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bioRxiv - Developmental Biology 2023Quote: 5’ - capped and 3’- polyadenylated hCas9-2xNLS mRNA was transcribed in vitro using mMessageMachine T7 (ThermoFisher, Cat# AM1344) and Poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...