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Citations for Thermo Fisher :
4901 - 4950 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... isotopologue patterns of labeled peptides were extracted manually from mass spectral data using the Xcalibur Qual Browser (v3.0.63, Thermo Fisher Scientific, Waltham, MA, USA), and 13C incorporation was calculated as previously described24.
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bioRxiv - Plant Biology 2021Quote: ... Five microliters of peptide mixtures were loaded onto an Acclaim PepMap™ precolumn (75 μm × 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm × 50 cm ...
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bioRxiv - Microbiology 2021Quote: LC-MS/SM analysis of trypsin-digested proteins (peptides) was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... dried at 30°C in a vacuum concentrator and fractionated using a Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were then labeled with TMT eleven-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled and desalted using a SepPak cartridge according to the manufacturer’s instructions (Waters ...
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bioRxiv - Biophysics 2022Quote: ... Peptides were dissolved in 25 μl 0.1% trifluoroacetic acid and 6 μl of the extracted peptides was analyzed by Orbitrap Q Exactive HF-X (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Biochemistry 2020Quote: LC-MS/MS analyses of all peptide mixtures were done using UltiMate™ 3000 RSLCnano system connected to Orbitrap Fusion Lumos Tribrid spectrometer (Thermo Fisher Scientific). Prior to LC separation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The eluted peptides from LC were subjected to mass spectrometry using a Q Exactive HF-X Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany). Protein identification was performed using Proteome Discoverer 2.4 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were delivered onto a nano RP column (5-μm Hypersil C18, 75 mm × 100 mm; Thermo Fisher Scientific, Waltham, MA, USA) and eluted with escalating (50-80% ...
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bioRxiv - Biochemistry 2021Quote: ... Desalted peptides were injected into an Easy-nLC 100 HPLC system coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Bremen, Germany). Peptide samples were loaded onto an Acclaim PepMapTM 100 C18 trap column (75 μm × 20 mm ...
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bioRxiv - Immunology 2021Quote: Cells were stimulated with 2 μg/mL of MTB300 epitope peptide pool (A&A Labs, USA) in the presence of brefeldin A and monensin (Thermo Fisher Scientific, USA) for 6 hours at 37°C in Complete media (RPMI-1640 with 10% fetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2022Quote: Protein digestion was performed according to the standard procedure and the resulting peptide mixture was labeled using the 10-plex TMT reagent (Thermo fisher, Art.No.90111) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... to the following protocol: Analysis of peptide readout was performed on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (Thermo Scientific) coupled with a Dionex Ultimate 3000 RS (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Tryptic peptides were directly injected to an EASY-Spray analytical column (2 μm, 100 Å PepMapRSLC C18, 25 cm × 75 μm, Thermo Fisher Scientific) operated at constant temperature of 35 °C ...
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bioRxiv - Cell Biology 2019Quote: ... a total of 1 ug of peptides was injected into a Dionex Ultimate 3000 NanoRSLC instrument with a 15-cm Acclaim PEPMAPC18 (Thermo Fisher Scientific #164534) reverse phase column ...
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bioRxiv - Biochemistry 2019Quote: ... These digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Synthetic Biology 2020Quote: Retention time was predicted after standard runs with the above-described method using the Pierce™ Peptide Retention Time Calibration Mixture (Catalog number 88320, Thermo Scientific, USA).
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bioRxiv - Cell Biology 2020Quote: ... the same maximal quantity of peptides was used for TMT labelling using a TMT10plex™ Isobaric Label Reagent Set (#90110, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The peptides were loaded on a 50 cm EASY-Spray™ column (75 μm inner diameter, 2 μm particle size, Thermo Fisher Scientific), equipped with an integrated electrospray emitter ...
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bioRxiv - Microbiology 2021Quote: ... PM-WCPV-S) or N peptide pool (Meltenyi, 130-126-698) in the presence of 1 µg/mL anti-CD28 (Invitrogen, 14-0281-86) for co-stimulation for 6 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were then analyzed with an nanoLC-MS/MS system (Ultimate nano-LC and LTQ Velos ion trap, Thermo Scientific, San Jose Califronia). Briefly ...
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bioRxiv - Physiology 2020Quote: ... The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo Fisher Scientific Inc., U.S.A) coupled online to the UPLC ...
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bioRxiv - Plant Biology 2021Quote: ... Analytical separation of peptides was performed on an Acclaim PepMap RSLC column (75 μm x 50 cm, C18, 3 μm, 100 Å; Thermo Fisher Scientific) at a flow rate of 300 nL min−1 ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 3 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL min−1 for 3 min ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were then loaded onto the main column (75-µm × 50-cm Acclaim PepMap100 C18, 3 µm, 100-Å, Thermo Fisher Scientific) with 95 % solvent A (0.1 % formic acid (v/v) ...
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bioRxiv - Biochemistry 2021Quote: Peptide identification and PTM relative quantitation were performed using the Thermo Scientific BioPharma Finder software version 4.1 (Thermo Scientific, San Jose, CA, USA) using parameters summarised in Table S1 ...
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bioRxiv - Physiology 2021Quote: Sample peptides were processed using an externally calibrated high-resolution electrospray tandem mass spectrometer (Thermo Q Exactive HF; Thermo Fisher Scientific, Waltham, MA) in conjunction with a Dionex UltiMate 3000 RSLCnano System (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Biochemistry 2021Quote: ... Analytical separation of peptides was done on an Acclaim PepMap RSLC column (75 μm x 50 cm, C18, 2 μm, 100 Å; Thermo Fisher Scientific) at a flow rate of 300 nL/min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Analytical separation of peptides was done on an Acclaim PepMap RSLC column (75 μm × 50 cm, C18, 2 μm, 100 Å; Thermo Fisher Scientific) at a flow rate of 300 nL/min ...
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bioRxiv - Genetics 2021Quote: ... Five microliters of peptide mixture were loaded onto an Acclaim PepMap™ precolumn (75 μm x 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm x 50 cm ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Desalted tryptic peptides were analyzed by liquid chromatography/tandem mass spectrometry (LCMS/MS) on nano-LC-QExative HF in FTFT (Thermo Fisher Scientific, www.thermofisher.com) interfaced with nano-Acquity LC system from Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Microbiology 2021Quote: ... Peptide sequences were determined by matching protein databases with the acquired fragmentation pattern by the Sequest software program (Thermo Fisher Scientific, Waltham, MA) 57 ...
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bioRxiv - Immunology 2021Quote: ... Then the peptides were transferred to an Acclaim PepMap RSLC C18 analytical column (75 μmx15 cmx2 μm, 100 Å diameter, Thermo Scientific, USA). The gradient for separation was 6-32% B in 80 min ...
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bioRxiv - Immunology 2021Quote: ... 0.9 μg of tryptic peptide samples label free shotgun proteomic data were obtained on an Orbitrap FusionTM TribridTM mass spectrometer (Thermo Fisher Scientific, USA) inline coupled to nanoACQUITY ultra performance liquid chromatography system (Waters ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were separated over a 25- cm EasySpray reversed phase LC column (75 μ m, 100 Å, PepMap C18 particles, Thermo Fisher Scientific). The mobile phases (A ...
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bioRxiv - Biochemistry 2020Quote: Peptides were analyzed using a LC-MS/MS platform of hybrid LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) equipped with a Waters nanoACQUITY ultra performance liquid chromatography (UPLC ...
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bioRxiv - Cancer Biology 2021Quote: ... The peptide mass was characterized by matrix assisted laser desorption/ionization (MALDI) on a Voyager DE Pro (Applied Biosystems Inc., Foster City, CA). The pure Def1 conjugate was air oxidized slowly with continuous stirring for 36-48 hrs and monitored the formation of S-S folding using MS/MS ...
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bioRxiv - Neuroscience 2021Quote: ... then combined into a single tube and fractionated into 12 samples using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher cat# 84868). Fractions 2 ...
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bioRxiv - Neuroscience 2021Quote: Whole mouse cortex was dissected from 9-month-old mouse brain and peptides were isolated using the EasyPep™ Mini MS Sample Prep Kit (Thermofisher cat# A40006) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... The resulting peptides were then labeled with TMT eleven-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled and desalted using a SepPak cartridge according to the manufacturer’s instructions (Waters ...
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bioRxiv - Microbiology 2022Quote: ... Lyophilized peptides were solubilized in 1ml PBS and incubated with 150µl pre-washed streptavidin agarose beads (catalog no. 20361; Thermo Fisher Scientific, MA, USA) for 2 hours at ambient temperature ...
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bioRxiv - Cancer Biology 2022Quote: The peptide samples prepared for building Nomo-1 sufaceome custom database were loaded on to the an EASY-Spray nanocolumn (Thermo Fisher Scientific, ES900) installed on Dionex Ultimate 3000 NanoRSLC instrument coupled with Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were separated by reverse phase liquid chromatography and eluted peptides were converted to gas-phase ions by electrospray and analyzed using an Orbitrap (Thermo Scientific, Waltham, MA) followed by Tandem MS to fragment the peptides through a quadropole for final mass detection ...
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bioRxiv - Molecular Biology 2022Quote: Oocytes were collected in porcine oocyte medium (Research Institute for the Functional Peptides, Yamagata, Japan) supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific Inc.) and 75 IU/mL FSH (Fuji Pharmaceuticals ...
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bioRxiv - Molecular Biology 2022Quote: Protein identifications in pooled peptide SEC fractions from in-lysate crosslinking were conducted using a Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific, Germany) connected to an Ultimate 3000 RSLCnano system (Dionex ...
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bioRxiv - Synthetic Biology 2022Quote: ... Digested peptides were then extracted and separated into 10 fractions by using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit according to the manufacturer’s protocol (Thermo Fisher Scientific, USA). Following fractionation ...
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bioRxiv - Plant Biology 2022Quote: ... except that peptides were separated on a PepMap™ RSLC C18 Easy-Spray column (75 µm × 50 cm, 2 µm, 100 Å; Thermo Scientific) with a 90 min gradient (0 to 20% B solvent (0.1% (v/v ...
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bioRxiv - Evolutionary Biology 2022Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...