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Citations for Thermo Fisher :
5101 - 5130 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2020Quote: ... Acidified eluted peptides were analyzed in the data-independent acquisition mode on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) online coupled to an ultra-high-performance liquid chromatography (UHPLC ...
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bioRxiv - Cell Biology 2022Quote: ... One aliquot containing 800 ng of Bradford-quantified peptides was analyzed on an ETD-enabled Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) connected to the EASY-nLC system (Proxeon Biosystem ...
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bioRxiv - Neuroscience 2022Quote: ... labeled peptides on one spin tip from a High-Select™ TiO2 Phosphopeptide Enrichment Kit (capacity of 1–3 mg; Thermo Fisher Scientific, catalog A32993). After preparing spin tips ...
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bioRxiv - Genetics 2022Quote: ... Sodium deoxycholate was removed by acidification with 10 % TFA and the sets were desalted using Pierce peptide desalting spin columns (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... the resulting peptides were labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled.
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bioRxiv - Pharmacology and Toxicology 2022Quote: The eluted peptides were ionized under high voltage (2 kV) and analyzed online using a LumosTM mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). The initial MS spectrum (MS1 ...
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bioRxiv - Cell Biology 2023Quote: Peptides were analyzed by LC-MS on a Dionex Ultimate HPLC coupled to an Orbitrap QE Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operating in Data Dependent Acquisition (DDA ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg of the peptide was used for LC-MS/MS analysis which was carried out using a nano-LC 1200 system (Thermo Fisher Scientific, San Jose, CA) coupled to Orbitrap Fusion™ Lumos ETD mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 25 cm EasySpray™ C18 column (2 μm, 100 Å, 75 μm x 25 cm, Thermo Scientific Cat No: ES902A) at 400 nL/min with a gradient of 4-30% with mobile phase B (Mobile phases A ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tryptic peptides from protein digests obtained from cell lines and tissue lysates were quantified by BCA using a peptide digest standard (Thermo Scientific, catalog no. 23225; 23295). Peptides were analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously [77 ...
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bioRxiv - Immunology 2023Quote: ... Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 6 µL of each sample and separated by high pH reversed phase peptide separation kit (Thermo Fisher Scientific, Waltham, MA, USA). Ten fractions were collected by centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking Buffer (E-cadherin mAb #14472 Cell Signaling Technology 1:1000, β-catenin mAb #8480 Cell Signaling Technology 1:1000, Occludin mAb #33-1500 Invitrogen 1:500). On the next day the membranes were washed three times for tenminutes in Tris-buffered saline + 0.05% Tween (#91414 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed three times in PBS and incubated during 4 h in a blocking solution containing a secondary antibody to reveal TH (donkey anti-rabbit Alexa Fluor 594, Invitrogen A21207 lot 1890862 and 2145022 (1:400), RRID ...
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bioRxiv - Molecular Biology 2022Quote: ... The slips were washed three times with PBS before the complementary anti-rabbit secondary antibody was added (Cat#A-11034, Life Technologies; 1:1000 dilution in blocking solution) and incubated for one hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat. A16110; Invitrogen cat. 31432; Invitrogen cat. 31402; R&D Biosystems cat. HAF016). We performed a final TBST wash (4 x 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... TFA and 1 μL of this dilution and 1μL of the undiluted peptides from bands 3 and 4 were injected onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm × 5 mm), using a partial loop injection ...
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bioRxiv - Biochemistry 2021Quote: Samples from imaging peptide extraction were dissolved in 0.1% FA and analyzed on the Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled to a Dionex UPLC system ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides (100 ng of acyl-BSA) were loaded onto an Acclaim PepMap100 C18 trap column (Thermo Scientific, Product #16-494-6, 75μm x 2cm, 100 Å) and separated on an Acclaim PepMap RSLC C18 analytical column (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the peptides were fractionated with high pH RP method and analyzed by a Q Exactive™ HF-X mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled with an online HPLC.
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bioRxiv - Neuroscience 2022Quote: ... One fifth of the phosphopeptides and one tenth of each global peptide fraction was loaded onto a reversed phase EasySpray™ C18 column (2 μm, 100 Å, 75 μm × 25 cm, Thermo Scientific Cat No: ES902A) at 400 nL/min ...
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bioRxiv - Microbiology 2023Quote: ... The separated peptides were ionized by a nano-ElectroSpray Ionization (ESI) and transferred to an Orbitrap Exploris™ 480 mass spectrometer (Thermo Fisher Scientific, San Jose, CA) for detection in DDA (Data dependent Acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... washed with PBS-T and then incubated with secondary antibodies for 2h (donkey anti-rabbit lgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206; donkey anti-Mouse lgG Alexa Fluor 555, Invitrogen, Thermo Fisher, A21202, 1:600 in blocking buffer). Sections were washed twice with PBS before being mounted onto gelatin subbed slides ...
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bioRxiv - Cancer Biology 2020Quote: The DDA acquisition of peptides from cell lines was performed on a nanoflow a nanoflow EASY-nLC™ 1200 System (Thermo Fisher Scientific™, San Jose, USA) coupled to a Q Exactive HF-X hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific™ ...
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bioRxiv - Physiology 2023Quote: ... samples were incubated with secondary antibodies diluted 1:500 and DAPI (1:1000) in blocking/permeabilisation buffer (Dk-a-Ch-AF488 – 703-545-155 Jackson Immunoresearch, Dk-a-Rb-AF546 – A10040 Thermofisher, Dk-a-Gt-AF647 – A-21447 Life Tech) for 1h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: On the Orbitrap setup comprised of a Dionex Ultimate 3000 nano LC with Thermo Q Exactive Classic peptides were separated on a 50-cm EasySpray column (Thermo Fisher, ES803, 2 μm beads, 75-μm ID) with a 60 minute gradient of 2 to 35% acetonitrile in 0.1% formic acid and 5% DMSO at a flow rate of 250 nL/min.
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend]; goat anti-myeloperoxidase [goatMPO, AF3667, R&D Systems], and [hamsteranti-CD31, 2H8, MA3105, Thermo Fisher] all diluted 1:200 in blocking buffer), for 3 days at 4°C on a shaker ...