Labshake search
Citations for Thermo Fisher :
4851 - 4900 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... coverslips were washed 3 x 10 min in PBS containing 0.1% Triton X-100 at room temperature and then incubated for 1 hr at room temperature with secondary antibodies diluted in blocking buffer: AlexaFluor594 goat anti-mouse IgG (Molecular Probes, ThermoFisher Scientific, AB_2534091) and AlexaFluor488 Streptavidin (Molecular Probes ...
-
bioRxiv - Bioengineering 2022Quote: ... cells were washed with 5 mL of PBS and incubated with a blocking buffer comprised of 1% bovine serum albumin (BSA, Fisher Scientific cat. no. BP9703) and 10% goat serum (Sigma Aldrich cat ...
-
bioRxiv - Cell Biology 2022Quote: ... then room temperature blocking solution (3% BSA in D-PBS) containing secondary antibody (IgG2b Cross-Adsorbed Goat anti-Mouse, Alexa Fluor™ 555 [Invitrogen, 10412832], 1:2000) was added using Dragonfly Discovery liquid dispenser and incubated for 1 h at room temperature ...
-
bioRxiv - Plant Biology 2022Quote: ... They were then treated with secondary antibody solution (1 : 200 of Alexa Fluor 488 goat anti-rabbit IgG; Molecular Probes, in the blocking buffer) for 2 hours at 37 °C and washed three times ...
-
bioRxiv - Microbiology 2022Quote: ... the coverslips were washed three times in PBS with 0.1% saponin and incubated for one hour at room temperature in blocking solution with 1.6 μg/ml DAPI dilactate (Molecular Probes, Thermo Fisher Scientific, Waltham, USA), 100 μg/ml Concanavalin A-Texas red conjugate (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... Slice were then incubated overnight at 4°C in blocking solution containing a rabbit anti-Parvalbumin (PV) antibody (1:400, cat # PA1933, Thermo Fisher Scientific, Waltham, MA). Slices were then washed and incubated blocking solution containing a secondary antibody donkey anti-rabbit Alexa568 diluted to 1:1000 (Abcam ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
-
bioRxiv - Synthetic Biology 2023Quote: ... cells were incubated overnight at 4°C in blocking buffer with the antibodies anti-HA tag conjugated to DyLight 550 (ThermoFisher Scientific, catalog #26183-D550) and anti-TIM23 (Abcam ...
-
LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... and then incubated in the same blocking solution containing secondary antibody (Donkey anti-rabbit Alexa fluorophore 647, 1:500 Thermo Fisher Scientific, Waltham, MA) for 1 hour at RT ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then washed 3 times with PBS and then blocked for 30 minutes at room temperature with antibody blocking buffer (150 mM NaCl, 50 mM Tris-Base, 1% BSA, 100 mM L-lysine 50% normal goat serum (Thermo Fisher, Cat# 01-6201), 0.2% Triton (Roche ...
-
bioRxiv - Biophysics 2023Quote: ... Secondary antibody staining was performed for 1 hour at RT in blocking solution (AF647 anti-rabbit #A21244, Thermo Fisher Scientific, 1:1.000; AF488 Phalloidin #A12379, Thermo Fisher Scientific, 1:1.000). Cells were washed trice for 10 min before mounting the coverslips on Marienfeld Superior Microscope slides with molds (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: Mouse peripheral blood was processed to remove RBC via lysis prior to Fc blocking and then stained with antibodies against surface markers including biotin-CD93 (Invitrogen, cat # 13-5892-85), BV605-CD19 (BD ...
-
bioRxiv - Developmental Biology 2023Quote: ... in 1x PBS and blocked for 1 hour (h) in blocking buffer made of washing buffer and 1% heat-inactivated goat serum (HIGS) (Life Technologies, Waltham, MA, USA). Skin sections were fixed in 4% PFA for 10 min before they were washed and blocked in blocking buffer containing 10% HIGS ...
-
bioRxiv - Neuroscience 2023Quote: Slices were stained for green fluorescent protein (GFP) with a rabbit monoclonal anti-GFP antibody (G10362, Molecular Probes, 1:1000 dilution in blocking buffer) and for tyrosine hydroxylase (TH ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were blocked with Mouse-on-Mouse IgG Blocking Solution for 1 h at room temperature (1:30; Thermo Fisher Scientific, Catalog no. R37621). Sections were incubated overnight at 4°C with primary antibodies—rabbit anti-Iba1 (1:1000 ...
-
bioRxiv - Immunology 2024Quote: ... Samples were washed in PBS-Tx and blocked for 1 hour in blocking buffer and stained with secondary antibody (Alexa Fluo-647, SA5-35500 Thermo Fisher Scientific, 1:500) for 2 hours ...
-
bioRxiv - Microbiology 2020Quote: ... The digestion was stopped with 4% formic acid (FA) (#94318, Fluka) and digested peptides were purified with C18 Spin Columns Pierce™ (#89870, ThermoFisher Scientific, USA). Peptides were eluted with 2 x 80% Acetonitrile (ACN ...
-
bioRxiv - Cell Biology 2020Quote: ... The eluted peptide samples were analyzed by a Q Exactive Plus™ hybrid quadrupole-Orbitrap™ mass spectrometer (Catalog No. IQLAAEGAAPFALGMAZR, ThermoFisher Scientific). The electrospray voltage was set to 2.0 kV ...
-
bioRxiv - Cell Biology 2020Quote: Each TMT labeled peptide set was fractionated to improve depth of proteome coverage using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific, Part 84868). The fractionation was performed according to the manufacturer’s protocol and yielded 8 peptide fractions for analysis ...
-
bioRxiv - Cell Biology 2020Quote: Nanoflow LC-MS analysis of tryptic peptides was performed using a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific 17) connected to an EASYnLC 1200 ultra-high-pressure system (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... The samples were subjected to tryptic digestion and the resulting peptide mixtures were analyzed by nano-ESI-LC-MS/MS (Thermo Scientific LTQ Orbitrap). Proteins were identified using Mascot (Matrix Science ...
-
bioRxiv - Biochemistry 2020Quote: ... and ~200 ng of peptides were injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... The resulting peptides were desalted and analyzed by LC-MS/MS on a Q-Exactive plus mass spectrometer (Thermo-Fisher scientific, Pittsburgh, PA) fitted with a capillary HPLC (easy nLC 1000 ...
-
bioRxiv - Biochemistry 2022Quote: ... Labeled peptides were first trapped on an Acclaim™ PepMap™ 100 C18 precolumn (5 μM, 0.3 mm X 5 mm, Thermo Fisher Scientific) and eluted to the analytical column nanoEase M/Z Peptide BEH C18 Column (130Å ...
-
bioRxiv - Neuroscience 2021Quote: ... Mass-spectrometry-based quantification of CaMKIIα/β-derived peptides was carried out by selected reaction monitoring (SRM) analysis using a TSQ Quantiva triple-stage quadrupole mass spectrometer (Thermo Fisher Scientific, U.S.A.). The following parameters were selected ...
-
bioRxiv - Molecular Biology 2021Quote: ... One microgram peptides for global proteomics or all the enriched phosphorylated peptides were loaded and concentrated on an Acclaim PepMap 100 C18 precolumn (75 μm x 2 cm, Thermo Scientific, Waltham, MA) and then separated on an Acclaim PepMap RSLC column (75 μm x 25 cm ...
-
bioRxiv - Cancer Biology 2020Quote: The PulseDIA acquisition of HeLa peptides was performed on a nanoflow EASY-nLC™ 1200 System (Thermo Fisher Scientific™, San Jose, USA) coupled to a Q Exactive HF-X hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific™ ...
-
bioRxiv - Molecular Biology 2019Quote: ... Eluted peptides were analyzed on a Q-Exactive Plus mass spectrometer or an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). The software Scaffold 4.7.2 (Proteome Software Inc. ...
-
bioRxiv - Biochemistry 2019Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany) and stored at −80 °C.
-
bioRxiv - Cancer Biology 2019Quote: ... The collected peptide was desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
-
bioRxiv - Microbiology 2021Quote: ... NanoUPLC-hr MS / MS analysis of the resulting peptide mixtures were performed on a Q-Exactive orbitrap mass spectrometer (Thermo Fisher Scientific, USA), coupled with a nanoUltimate300 UHPLC system (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Tagged tryptic peptides (~250 μg) were subjected to strong cation exchange fractionation using a hand-held ICAT® Cartridge-cation-exchange system (Applied Biosystems, USA). Peptides were eluted using a gradient 30 ...
-
bioRxiv - Microbiology 2020Quote: 50µg of peptides from each sample were labeled with isobaric tandem-mass-tag (TMT) 11 plex reagents (Thermo Fisher Scientific, San Jose, CA) in 20 mM pH 8.5 HEPES with 30% acetonitrile (v/v ...
-
bioRxiv - Microbiology 2020Quote: The flow-through fraction of each TMT11 experiment was fractionated into 13 fractions using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
-
bioRxiv - Bioengineering 2021Quote: ... the UV absorbance of the NPs was measured at 205 nm before and after peptide coupling using a spectrophotometer (Nanodrop 2000, Thermo Fisher Scientific, UK) and the difference of the absorbance (ΔA205 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The separated peptides were then electrosprayed in the positive ion mode into an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher, San Jose, CA, USA) operated in data-dependent acquisition mode (3 s cycle time) ...
-
bioRxiv - Biochemistry 2020Quote: ... and with or without CLASPIN pS945 peptide (MEELLNLC(pS)GKFTSQ) (Peptide Protein Research Ltd, Bishops Waltham, UK) at different concentrations in a Thermoscientific 96 well UV microplate (Fisher scientific, Loughborough, UK). The plates were incubated at 25 °C and absorbance at 340 nm was measured in a POLARstar Omega multimode microplate reader (BMG Labtech GmbH ...
-
bioRxiv - Biochemistry 2020Quote: ... The peptide samples of EMARS products prepared as well as mouse samples were injected into an Ultimate 3000 RSLCnano (Thermo Fisher Scientific, MA). Mass analysis was performed on a LTQ Orbitrap XL mass spectrometer equipped with a nano-ESI source (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... operated in the cone-jet spraying regime for efficient ion generation.33 Peptide ions were detected using a quadrupole orbitrap mass spectrometer (Q Exactive Plus, Thermo Scientific, Waltham, MA) between m/z 350–1,800 at 35,000 FWHM resolution (128 ms transient ...
-
bioRxiv - Neuroscience 2021Quote: Eluted peptides were ionized by a nanospray source and detected by an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operating in a data dependent mode (DDA) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Tagged tryptic peptides (∼250 μg) were subjected to strong cation exchange fractionation using a hand-held ICAT® Cartridge-cation-exchange system (Applied Biosystems, USA).
-
bioRxiv - Microbiology 2020Quote: ... Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid ...
-
bioRxiv - Plant Biology 2020Quote: The coding DNA sequence for human Factor IX without the native signal peptide was synthesized as N-terminal translational fusion to the signal-peptide coding sequence of PpAP1 (Pp3c5_19520V3.1)42 by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA). It was cloned into the pAct5-MFHR1 plasmid41 to generate the final FIX expression vector ...
-
bioRxiv - Systems Biology 2020Quote: ... fractionation for diGly peptide and proteome spectral libraries were performed on an UFLC system (Shimadzu) and EASY-nLC 1000 (Thermo Fisher Scientific, Germany), respectively.
-
bioRxiv - Systems Biology 2021Quote: The concatenated and TMT-labelled peptide mixtures were analyzed using nanoflow liquid chromatography (LC-MS/MS) on an EASY nano-LC 1200™ system (Thermo Fisher scientific), connected to a Q Exactive HF (Thermo Fisher scientific ...
-
bioRxiv - Systems Biology 2021Quote: ... Air dried peptides were analyzed using a nanoflow liquid chromatography (LC-MS/MS) on an EASY nano-LC 1200™ system (Thermo Fisher scientific), connected to a Q Exactive HF (Thermo Fisher scientific ...
-
bioRxiv - Microbiology 2020Quote: Liquid chromatography coupled to mass spectrometry (LC-MS/MS) analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2020Quote: ... The soluble fraction was then added to a new tube and the peptides were extracted and desalted using C18 desalting columns (Thermo Scientific, PI-87782). Peptides were quantified using BCA assay and a total of 1 ug of peptides were injected for LC-MS analysis.
-
bioRxiv - Microbiology 2021Quote: ... and then tryptic peptides were subjected to LC-MS/MS on an Easy-Nlc 1000 coupled with Q Exactive Orbitrap instrument (Thermo Fisher Scientific, USA) under standard conditions ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting peptides were then labelled with TMT ten-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled and desalted using a SepPak cartridge according to the manufacturer’s instructions (Waters ...