Labshake search
Citations for Thermo Fisher :
4801 - 4850 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Optical density was measured at 450 nanometer using a Multiskan FC machine (Thermo Scientific, USA) in combination with Mikrowin (software version 5.09 ...
-
bioRxiv - Cancer Biology 2023Quote: Hap1 cells (Horizon Discovery) were maintained in IMDM supplemented with GlutaMAX and 10% FCS (Gibco). Cells regularly tested negative for mycoplasma contamination.
-
bioRxiv - Immunology 2023Quote: ... Pheonix cell and LS174T cells were cultured in complete DMEM (Gibco, 10% FCS, 2mM GlutaMAX).
-
bioRxiv - Immunology 2023Quote: ... Samples were then incubated with an Fc receptor binding inhibitor polyclonal antibody (Thermo Fisher Scientific), before staining with a surface antibody cocktail (Supplementary Table 1) ...
-
bioRxiv - Immunology 2024Quote: ... followed by Fc-Block step with anti-mo CD16/CD32 (Invitrogen, Cat# 14-01061-86) and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD ...
-
bioRxiv - Plant Biology 2024Quote: ... and reading absorbance at 620 nm in a photometer (Multiskan FC, Thermo Scientific, United States) and using a glucose standard curve to compare the colorimetric response of the samples ...
-
bioRxiv - Immunology 2024Quote: ... followed by absorbance measurements at 450nm using a Multiskan FC Microplate Photometer (51119000; Thermo Scientific). As a positive control ...
-
bioRxiv - Immunology 2024Quote: ... Fc receptors were then blocked with anti-mouse CD16/CD32 (Invitrogen, Cat# 14-01061-86) along with extracellular staining with a mixture of conjugated antibodies in FACS buffer (1:250 for LP cells ...
-
bioRxiv - Immunology 2024Quote: ... Absorbance was detected at 450 nm by the Multiscan FC (Thermo Fisher, Waltham, MA, USA).
-
bioRxiv - Immunology 2024Quote: ... + 10% FCS + 1% GlutaMAX + penicillin-streptomycin (P/S, 12.8 U/mL, Gibco, cat.15140-122) supplemented with 10% DMSO at -150°C ...
-
bioRxiv - Immunology 2024Quote: ... containing 10% FCS (Biochrome, Cat. S0615-500ML) and 1% Penicillin/ Streptomycin (Thermo Fisher, Cat. 7001592). After sorting ...
-
bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
-
bioRxiv - Genomics 2020Quote: ... embryos were incubated with primary antibodies at 1:375 dilution (sheep anti-DIG, Roche cat #11333089001 and mouse anti-Biotin, Life technologies cat #03–3700) overnight at 4°C ...
-
bioRxiv - Genomics 2020Quote: ... then 2 h with secondary antibodies at 1:500 dilution (anti-mouse Alexa488-conjugated Life technologies cat #A21202 and anti-sheep Alexa555-conjugated Life technologies cat #A21436) and washed 6 times in PBT ...
-
bioRxiv - Neuroscience 2023Quote: ... specific fluorescent immunostainings were visualized using Alexa488-conjugated anti-sheep IgG and Alexa546-conjugated anti-rabbit IgG (1:300; Molecular Probes, Eugene, OR, USA).
-
bioRxiv - Bioengineering 2022Quote: ... were used to normalize expression levels of eight osteogenic marker genes (RUNX2, BGLAP, DMP1, ALPL, PDPN, SOST, COL1a2, MMP14) which were analyzed with specific TaqMan probes (Applied Biosystems, see Table S1).
-
bioRxiv - Cell Biology 2022Quote: Primary human lung tissue from post-mortem or surgical resection donors (detailed in supplemental table S1) was fixed in 10% neutral buffered Formalin (Thermo Fisher Scientific, Waltham, MA). The tissue was then dehydrated in 70% ethanol (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... were plated into each well of a 24-well plate and transfected with 50 ng pCAG-hyPBase (Ochiai et al., 2015) and 450 ng Intein-related plasmids (Tables S1 and S2) using Lipofectamine 3000 (L3000015, Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: Coding sequences of mature proteins without signal peptide were amplified with primers listed in Table S1 and cloned into expression vector pETSUMO (Thermo Fisher Scientific, Massachusetts, USA). Correctness of the resulting constructs pETSUMO-ChElp2 and pETSUMO-Vd2LysM were confirmed by DNA sequencing and introduced into E ...
-
bioRxiv - Systems Biology 2022Quote: The Headspace-SESI system consists of an in-house developed custom-made headspace sampling system (Fig 1C and Fig S1, adapted from [61]), a commercial SESI source (SuperSESI, Fossiliontech, Spain) and an Orbitrap mass spectrometer (Q-Exactive Plus, Thermo Scientific, San Jose, CA). All three EAM strains were able to grow in the Headspace-SESI system under the measurement condition (Fig S4A) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... cells were transfected with the PiggyBac or Tol2 transposase expression vector (mPBase or pCAGGS-T2TP; Table S1) and the donor vectors using 293fectin (Thermo Fisher Scientific, Waltham, MA). One day after tge transfections ...
-
bioRxiv - Developmental Biology 2019Quote: ... A single-stranded oligonucleotide with 50 bp arms for HDR (Supplementary Table S1) was also delivered with PX459 using the Neon® Transfection System (Thermo Fisher, New Zealand) at 1,500 V with one 20 ms pulse ...
-
bioRxiv - Plant Biology 2021Quote: ... fragments were purified and used as templates for in vitro transcription. The products of RNA transcripts were confirmed by gel electrophoresis (Supplementary Fig. S1) and quantified by NanoDrop (Thermo Fisher Scientific, Waltham, USA). We equally mixed 3 fragments and used for SIGS assay on polyethylene tape ...
-
bioRxiv - Physiology 2023Quote: ... The gene expression level relative to β-actin was determined by qPCR with gene-specific primers (Supplementary Table S1) and PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, USA). 2-ΔΔCT was calculated for relative fold change.
-
bioRxiv - Microbiology 2024Quote: ... ssRNA/dsRNA fractionation was performed as previously described 48. To visualize dsRNA, 1 µg were pretreated with RNase-free DNase1 (RBC Bioscience, cat. #9680DN050) and S1 nuclease (Thermo Scientific™, cat. #EN0321) according to the manufacturer’s instructions and electrophoresed on 1% agarose gel stained with ethidium bromide.
-
bioRxiv - Neuroscience 2023Quote: ... Blots were incubated with HRP-conjugated secondary antibodies at 1:10000 for 1h at room temperature (see Table S1 for suppliers) and developed with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher®, Waltham, US) on CL-X Posure™ X-ray films (34091 ...
-
bioRxiv - Cancer Biology 2023Quote: ... About 10-100 ng genomic DNA with 2.5 μM primers targeting the promoter region of PTEN or ATAD1 and a genomic region on Chr 12 as an internal control (Supplementary Table S1) and PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™) were mixed ...
-
bioRxiv - Molecular Biology 2023Quote: ... The rfc3 gene was amplified from Xenopus egg cDNA by two-step PCR using primers listed in Supporting Table S1 and cloned into pDONR201 (Thermo Fisher Scientific, MA, USA) by the Gateway BP reaction ...
-
bioRxiv - Genomics 2023Quote: Lipofectamine 3000-DNA transfection complexes were prepared by diluting 10ug of pHAGE-EF1a-BRD4-NUT DNA (Datafile S1) or pUC19 (negative control) in 500ul of DMEM/F12 media (ThermoFisher, catalog no. 11330-032), mixing well and incubating for 1 min ...
-
bioRxiv - Plant Biology 2024Quote: ... The sequence encoding mouse MmCyp1a2 and maize ZmCYP81A9 were codon-optimised for in planta expression and synthesized as a GeneString (GeneArt, ThermoFisher Scientific, Supplemental Table S1). Using BpiI restriction sites ...
-
bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
-
bioRxiv - Cell Biology 2020Quote: ... Secondary goat anti-mouse IgG and rabbit anti-goat IgG conjugated to horseradish peroxide (ECL; Thermo Fisher Scientific), or a fluorophore specific to the Odyssee system (IRDye 680RD and IRDye 800RD ...
-
bioRxiv - Molecular Biology 2023Quote: ... cRNA was prepared according to the manufacturer’s instructions from linearized plasmids encoding SARS-COV2 Protein E using the mMESSAGE mMACHINE T7 kit (Ambion, TX, USA). RNA concentrations were quantified by UV spectroscopy (NanoDrop ...
-
bioRxiv - Molecular Biology 2021Quote: ... anti-afadin (clone D1Y3Z) or Alexa Fluor 488 anti-DYKDDDDK (flag) tag monoclonal antibody (L5, Life Technologies). The plasma membrane was stained with CF594 dye conjugated wheat germ agglutinin (Biotium) ...
-
bioRxiv - Cell Biology 2019Quote: ... and pENTR-Megator constructs with pHWG (blasticidinR; C-terminal EGFP tag) using Gateway LR Clonase II (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysates were immunoprecipitated by incubating with an antibody to the c-Myc tag (Invitrogen 13-2500) on BBS2 as described above and immunoblotted with the c-Myc antibody for BBS2 and with the anti-Flag antibody (Sigma F3165 ...
-
bioRxiv - Neuroscience 2020Quote: ... Tandem Mass Tags (TMT 10-plex) kits and microBCA protein assay kit were purchased from Thermo Fisher Scientific (Rockford ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were stained with anti-GFP antibody (5 μg/ml, Thermo Fisher, RFP Tag Monoclonal Antibody RF5R) and whole node confocal scans were used to quantify percent metastatic area of total node via image thresholding in ImageJ ...
-
bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
-
bioRxiv - Biophysics 2020Quote: ... the kdpFABC operon with the 8x histidine tag was cloned into the pBAD vector (Invitrogen, Carlsbad CA) and introduced into E ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pierce Trypsin/Lys-C Protease Mix and tandem mass tag (TMT)-labeling kit were from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were purified via affinity pulldown of their StrepII tags using a StrepTrap HP column (Fisher Scientific) and eluted into Buffer A supplemented with 2.5mM desthiobiotin (Fisher Scientific) ...
-
bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
-
bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tandem Mass Tag™ 6-plex (TMTsixplex™) reagents kits for isotopic labeling were from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Microbiology 2022Quote: ... Desalted peptide samples from each sample were labeled with 10-plex tandem mass tags (TMT, ThermoFisher Scientific) with one of the TMT channels (131 ...
-
bioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) eleven plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
-
bioRxiv - Physiology 2022Quote: ... 25 μg of peptides per sample were labeled with isobaric tandem mass tags (TMT10plex, Thermo Fisher Scientific) as described previously [48] ...